##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693952
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.653878078022686	34.0	31.0	34.0	31.0	34.0
2	32.82323561283778	34.0	31.0	34.0	31.0	34.0
3	32.89889502443973	34.0	31.0	34.0	31.0	34.0
4	36.331933620769156	37.0	37.0	37.0	35.0	37.0
5	36.18858941252421	37.0	35.0	37.0	35.0	37.0
6	36.213157394171354	37.0	36.0	37.0	35.0	37.0
7	36.19418345937471	37.0	36.0	37.0	35.0	37.0
8	36.182274854744996	37.0	36.0	37.0	35.0	37.0
9	37.93642499769437	39.0	38.0	39.0	35.0	39.0
10	37.90187793968459	39.0	38.0	39.0	35.0	39.0
11	37.9693725214424	39.0	38.0	39.0	35.0	39.0
12	37.936207403393894	39.0	38.0	39.0	35.0	39.0
13	37.9482889306465	39.0	38.0	39.0	35.0	39.0
14	39.33182698515171	40.0	39.0	41.0	36.0	41.0
15	39.335938220510926	40.0	39.0	41.0	36.0	41.0
16	39.32766675504934	40.0	39.0	41.0	36.0	41.0
17	39.30932975191368	40.0	39.0	41.0	36.0	41.0
18	39.30256559531495	40.0	39.0	41.0	36.0	41.0
19	39.33401589735313	40.0	39.0	41.0	36.0	41.0
20	39.30856024624182	40.0	39.0	41.0	36.0	41.0
21	39.27815036198469	40.0	39.0	41.0	36.0	41.0
22	39.235141623628145	40.0	39.0	41.0	36.0	41.0
23	39.186319803098776	40.0	39.0	41.0	36.0	41.0
24	39.14261937425067	40.0	39.0	41.0	36.0	41.0
25	39.093038135202434	40.0	39.0	41.0	36.0	41.0
26	38.994796470072856	40.0	39.0	41.0	36.0	41.0
27	38.91803323572812	40.0	38.0	41.0	35.0	41.0
28	38.85605920870608	40.0	38.0	41.0	35.0	41.0
29	38.79111523563589	40.0	38.0	41.0	35.0	41.0
30	38.72499538873006	40.0	38.0	41.0	35.0	41.0
31	38.65184479618187	40.0	38.0	41.0	35.0	41.0
32	38.573415740569956	40.0	38.0	41.0	35.0	41.0
33	38.49419124089274	40.0	38.0	41.0	34.0	41.0
34	38.41482984413908	40.0	38.0	41.0	34.0	41.0
35	38.38577307940607	40.0	38.0	41.0	34.0	41.0
36	38.3527218020843	40.0	38.0	41.0	34.0	41.0
37	38.29637208337176	40.0	38.0	41.0	34.0	41.0
38	38.24252109655999	40.0	38.0	41.0	34.0	41.0
39	38.18268410495251	40.0	38.0	41.0	34.0	41.0
40	38.113131743982294	40.0	38.0	41.0	34.0	41.0
41	38.05426744212856	40.0	37.0	41.0	34.0	41.0
42	37.996106358941255	40.0	37.0	41.0	33.0	41.0
43	37.13902690676012	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	3.0
17	10.0
18	13.0
19	28.0
20	63.0
21	108.0
22	196.0
23	313.0
24	507.0
25	810.0
26	1251.0
27	1843.0
28	2646.0
29	3809.0
30	5449.0
31	6932.0
32	9620.0
33	12851.0
34	18476.0
35	27000.0
36	42708.0
37	78265.0
38	188479.0
39	292570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.465184911924744	18.38945633127363	12.57291570598543	26.572443050816197
2	19.641704325371208	20.305438992898644	33.539927833625384	26.512928848104767
3	20.71382458729134	21.46056211380614	29.06137600295121	28.764237295951308
4	15.825302038181317	15.589406760121738	32.62934612192198	35.95594507977497
5	16.645387577238772	34.153082633957396	32.718977681453474	16.482552107350365
6	35.71774416674352	32.98599903163331	15.429165129576687	15.867091672046483
7	29.971093101540163	29.000276676196624	20.633847874204555	20.394782348058655
8	27.299870884441575	32.09717674997694	19.91708360232408	20.6858687632574
9	26.598237342064003	14.295657336530482	19.6427130406714	39.46339228073412
10	18.068108457069076	25.76273286913216	32.04919072212487	24.11996795167389
11	36.58379830305266	21.012404316148668	20.870607765378583	21.53318961542009
12	22.36840588398045	24.48714032094439	27.99819007654708	25.146263718528083
13	31.021309831227523	18.825797749700268	23.841850733191922	26.311041685880294
14	23.671089643087704	20.393197224015495	23.761585815733653	32.17412731716315
15	26.32429908696855	26.601695794521813	21.31876556303606	25.755239555473576
16	26.16650719358111	25.16153855021673	23.17696324817855	25.49499100802361
17	24.904748455224567	25.494414599280642	24.34793760951766	25.25289933597713
18	24.742057087521903	24.0956146822835	25.67036336807157	25.49196486212303
19	25.627565018906207	24.534982246610717	25.290798210827266	24.546654523655814
20	25.662581850041498	24.04561122383104	25.18156875403486	25.110238172092593
21	26.1640574564235	24.39794106797012	24.362347828091856	25.075653647514525
22	25.995602001291157	24.154408374066218	24.276751129761138	25.57323849488149
23	25.22105275292816	24.225738956008485	24.793645670017522	25.759562621045834
24	25.572806188324265	24.542187355897816	24.52950636355252	25.355500092225398
25	25.74558470902887	23.895456746287927	24.5809508438624	25.778007700820805
26	25.409106105321406	24.65559577607673	24.63268352854376	25.302614590058102
27	25.757545190445448	23.99834570690768	24.29476390297888	25.949345199667988
28	24.953887300562574	24.379640090380892	25.08069722401549	25.585775385041043
29	24.766554459098035	24.6668357465646	25.21802660702758	25.348583187309785
30	24.71583048971687	25.098709997233236	25.14078783546989	25.044671677580006
31	25.660564419441112	24.422582541731995	24.612941529097114	25.303911509729783
32	24.635709674444342	24.488581342801808	24.827653785852625	26.048055196901228
33	24.607177441667435	24.064200405791755	25.2301311906299	26.09849096191091
34	25.429424513511023	23.66229940975745	25.176957484091115	25.731318592640413
35	25.12436018629531	23.598606243659503	25.740685234713638	25.53634833533155
36	24.412495388730058	23.732765378585263	25.83910702757539	26.015632205109284
37	24.91814995849857	22.631824679516736	26.21334040394725	26.236684958037443
38	24.152102739094346	22.61856727842848	27.418034676749976	25.8112953057272
39	23.996328276307295	22.245198515171076	27.710994420363367	26.04747878815826
40	23.519638245872915	22.353131052291804	28.46810730425159	25.659123397583695
41	22.722609056534168	22.51769574840911	28.989613114451718	25.770082080605
42	23.013695471732916	22.461784100341234	28.740460435303884	25.784059992621966
43	22.120982431061513	22.253700544129853	29.16051830674168	26.464798718066955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	61.0
2	58.0
3	126.5
4	195.0
5	195.0
6	254.0
7	313.0
8	314.5
9	316.0
10	485.5
11	655.0
12	655.0
13	1077.5
14	1500.0
15	2138.5
16	2777.0
17	2768.5
18	2760.0
19	2760.0
20	3309.5
21	3859.0
22	4229.5
23	4600.0
24	5494.5
25	6389.0
26	6389.0
27	7256.5
28	8124.0
29	10227.0
30	12330.0
31	14171.0
32	16012.0
33	16012.0
34	18266.5
35	20521.0
36	23360.5
37	26200.0
38	29468.5
39	32737.0
40	32737.0
41	35341.0
42	37945.0
43	38595.0
44	39245.0
45	41803.0
46	44361.0
47	44361.0
48	46437.5
49	48514.0
50	50578.5
51	52643.0
52	54493.0
53	56343.0
54	56343.0
55	53177.0
56	50011.0
57	48661.0
58	47311.0
59	44794.0
60	42277.0
61	42277.0
62	39412.5
63	36548.0
64	32897.5
65	29247.0
66	25878.5
67	22510.0
68	22510.0
69	19455.0
70	16400.0
71	14192.5
72	11985.0
73	9699.0
74	7413.0
75	7413.0
76	6026.5
77	4640.0
78	3768.0
79	2896.0
80	2388.5
81	1881.0
82	1881.0
83	1435.5
84	990.0
85	847.5
86	705.0
87	573.5
88	442.0
89	442.0
90	320.5
91	199.0
92	112.0
93	25.0
94	17.5
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	693952.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.36577765115867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6028109962972	60.36163042495352
2	5.9296168641348785	7.989065024517419
3	1.592542971110671	3.2184868707527117
4	0.7414274302850733	1.9978734165221679
5	0.42700398236929904	1.4382727666224737
6	0.28812773239247547	1.164596925329043
7	0.19796911589489286	0.9335440410220269
8	0.1458142079587671	0.7858310009384091
9	0.1105111543643538	0.6700202867593738
>10	0.805486565011334	10.86546287814738
>50	0.110141478060631	5.141819337353503
>100	0.04790590517609556	5.026536973433372
>500	2.138656480799706E-4	0.1120882159131392
>1k	4.277312961599412E-4	0.294771837735582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1031	0.1485693534999539	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1015	0.14626371852808265	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	778	0.1121115005072397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.4410218574195332E-4
9	0.0	0.0	0.0	1.4410218574195332E-4	1.4410218574195332E-4
10	0.0	0.0	0.0	2.8820437148390665E-4	1.4410218574195332E-4
11	0.0	0.0	0.0	4.3230655722586E-4	1.4410218574195332E-4
12	0.0	0.0	0.0	4.3230655722586E-4	1.4410218574195332E-4
13	0.0	0.0	0.0	5.764087429678133E-4	1.4410218574195332E-4
14	0.0	0.0	0.0	7.205109287097667E-4	1.4410218574195332E-4
15	0.0	0.0	0.0	0.00129691967167758	1.4410218574195332E-4
16	0.0	0.0	0.0	0.0018733284146453934	1.4410218574195332E-4
17	0.0	0.0	0.0	0.002017430600387347	1.4410218574195332E-4
18	0.0	0.0	0.0	0.002305634971871253	1.4410218574195332E-4
19	0.0	0.0	0.0	0.0024497371576132068	1.4410218574195332E-4
20	0.0	0.0	0.0	0.002737941529097113	1.4410218574195332E-4
21	0.0	0.0	0.0	0.00302614590058102	1.4410218574195332E-4
22	0.0	0.0	0.0	0.003602554643548833	1.4410218574195332E-4
23	0.0	0.0	0.0	0.00518767868671032	1.4410218574195332E-4
24	0.0	0.0	0.0	0.00778151803006548	1.4410218574195332E-4
25	0.0	0.0	0.0	0.01037535737342064	1.4410218574195332E-4
26	0.0	0.0	0.0	0.017148160103292447	1.4410218574195332E-4
27	0.0	0.0	0.0	0.043518860094069904	2.8820437148390665E-4
28	0.0	0.0	0.0	0.12724223001014479	2.8820437148390665E-4
29	0.0	0.0	0.0	0.22998708844415752	2.8820437148390665E-4
30	0.0	0.0	0.0	0.3622728949552707	2.8820437148390665E-4
31	0.0	0.0	0.0	0.7415498478280919	2.8820437148390665E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTA	25	0.0054948605	29.6	19
GGTATCA	460	0.0	22.119566	1
TATACAC	230	0.0	20.913044	36
TGGACTA	55	5.1402726E-4	20.181818	5
CAGTATG	55	5.1402726E-4	20.181818	4
TATATTG	50	0.0070321406	18.5	5
GTATAGA	50	0.0070321406	18.5	1
CCTTTAG	95	3.602223E-6	17.526316	1
ACAGCGC	75	2.0657794E-4	17.266666	8
TCTAGCG	65	0.0015790809	17.076923	28
GAGCGTC	65	0.0015790809	17.076923	7
TAACGCC	110	7.7966433E-7	16.818182	4
TCTTATA	1020	0.0	16.686274	37
CTTATAC	555	0.0	16.333334	37
GATCAAG	105	9.332351E-6	15.857142	1
ATCTCGC	145	5.3341864E-8	15.310345	11
CGACTTT	135	3.9652514E-7	15.074075	35
ATACACA	310	0.0	14.919354	37
ATCCTAA	75	0.004102227	14.8	1
CTAGCAC	100	1.09237204E-4	14.8	3
>>END_MODULE