##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632092.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449765
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69275288206063	34.0	31.0	34.0	31.0	34.0
2	32.85539781886096	34.0	31.0	34.0	31.0	34.0
3	32.941625070870344	34.0	31.0	34.0	31.0	34.0
4	36.368430180205216	37.0	37.0	37.0	35.0	37.0
5	36.223705712983445	37.0	37.0	37.0	35.0	37.0
6	36.25180260802864	37.0	37.0	37.0	35.0	37.0
7	36.22644714461997	37.0	36.0	37.0	35.0	37.0
8	36.21483219014374	37.0	36.0	37.0	35.0	37.0
9	38.00414660989628	39.0	38.0	39.0	35.0	39.0
10	37.942218714217425	39.0	38.0	39.0	35.0	39.0
11	38.016168443520506	39.0	38.0	39.0	35.0	39.0
12	37.97540271030427	39.0	38.0	39.0	35.0	39.0
13	38.00315498093449	39.0	38.0	39.0	35.0	39.0
14	39.376752303980965	40.0	39.0	41.0	37.0	41.0
15	39.39195135237291	40.0	39.0	41.0	37.0	41.0
16	39.384692005825265	40.0	39.0	41.0	37.0	41.0
17	39.37439996442587	40.0	39.0	41.0	37.0	41.0
18	39.363189665714316	40.0	39.0	41.0	37.0	41.0
19	39.39874823518949	40.0	39.0	41.0	37.0	41.0
20	39.37526708392161	40.0	39.0	41.0	37.0	41.0
21	39.35254188298334	40.0	39.0	41.0	37.0	41.0
22	39.314435316220695	40.0	39.0	41.0	36.0	41.0
23	39.27666003357309	40.0	39.0	41.0	36.0	41.0
24	39.23181216857692	40.0	39.0	41.0	36.0	41.0
25	39.17354396184674	40.0	39.0	41.0	36.0	41.0
26	39.08944671106022	40.0	39.0	41.0	36.0	41.0
27	39.01955687970384	40.0	39.0	41.0	36.0	41.0
28	38.95383144531033	40.0	39.0	41.0	35.0	41.0
29	38.89880715484753	40.0	38.0	41.0	35.0	41.0
30	38.83683701488555	40.0	38.0	41.0	35.0	41.0
31	38.765315220170535	40.0	38.0	41.0	35.0	41.0
32	38.68506442253177	40.0	38.0	41.0	35.0	41.0
33	38.62114659877936	40.0	38.0	41.0	35.0	41.0
34	38.54383511389281	40.0	38.0	41.0	35.0	41.0
35	38.51946016252932	40.0	38.0	41.0	35.0	41.0
36	38.4821295565462	40.0	38.0	41.0	34.0	41.0
37	38.43225239847476	40.0	38.0	41.0	34.0	41.0
38	38.380638778028526	40.0	38.0	41.0	34.0	41.0
39	38.33526174780163	40.0	38.0	41.0	34.0	41.0
40	38.27258901870977	40.0	38.0	41.0	34.0	41.0
41	38.22885506875813	40.0	38.0	41.0	34.0	41.0
42	38.17924916345203	40.0	38.0	41.0	34.0	41.0
43	37.290785187820305	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	5.0
19	12.0
20	29.0
21	65.0
22	93.0
23	183.0
24	277.0
25	464.0
26	662.0
27	987.0
28	1517.0
29	2164.0
30	2971.0
31	4085.0
32	5466.0
33	7783.0
34	11494.0
35	16939.0
36	26960.0
37	49441.0
38	122610.0
39	195554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07894122486187	17.7755049859371	12.012940090936377	27.132613698264652
2	18.604604626860695	20.408213177992952	33.91571153824775	27.071470656898605
3	19.54309472724645	21.53435683078941	30.350516380776625	28.572032061187507
4	14.875990795193045	15.227952375129233	34.289239936411235	35.60681689326648
5	15.423387769168343	35.544784498571474	33.85990461685547	15.171923115404711
6	35.335786466265716	34.57272131001745	15.578802263404224	14.512689960312608
7	30.322279412582127	29.942303202783677	20.61676653363423	19.118650850999966
8	28.147365846608785	32.842484408524456	19.837915355796916	19.172234389069846
9	27.338721332251286	13.774082020610765	18.462752770891466	40.424443876246485
10	16.960857336609116	26.381777150289597	32.462508198726006	24.194857314375284
11	37.2756884150612	20.833546407568395	20.845552677509367	21.045212499861037
12	22.781897212989005	24.37228330350294	28.387046568763687	24.45877291474437
13	31.49200137849766	18.34758151479106	24.117483574755706	26.042933531955576
14	23.65301879870599	19.330539281624848	23.85934877102487	33.15709314864429
15	26.098740453347858	26.416684268451302	21.36226696163552	26.122308316565316
16	26.212577679454824	25.346347537047127	22.60158082554223	25.839493957955824
17	24.992829588785252	25.490422776338757	24.076795659955756	25.439951974920238
18	24.90967505252743	24.251553589096527	25.37703022689627	25.46174113147977
19	26.100296821673542	24.3947394750592	24.822073749624803	24.68288995364246
20	26.13965070648005	23.74484452992118	24.817626982980002	25.297877780618766
21	26.50361855635721	24.2940202105544	23.62589352217269	25.5764677109157
22	26.44203083832668	24.066345758340464	23.74373283825998	25.747890565072872
23	25.6284948806599	24.21864751592498	24.093915711538248	26.05894189187687
24	25.83415783798206	24.206863584316256	24.24799617578069	25.710982401921
25	25.86862027947928	23.759963536513514	24.365835491867976	26.00558069213923
26	25.819038831389722	24.10947939479506	24.252887619089968	25.818594154725243
27	25.923982524207084	23.965404155503432	24.05856391671206	26.052049403577428
28	25.395706646804445	24.18618611941792	24.518137249452494	25.89996998432515
29	25.005058197058467	24.548597600969398	24.656209353773637	25.790134848198505
30	24.858092559447712	25.269640812424264	24.454770824764044	25.417495803363977
31	25.646948962235832	24.600402432381355	24.148166264604846	25.604482340777963
32	24.714684335152803	24.50079485953776	24.583282380798863	26.201238424510574
33	24.81962802797016	24.107700688137136	25.017064466999432	26.055606816893267
34	25.48264093471035	23.809544984603072	24.956143763965628	25.751670316720954
35	25.405489533423008	23.948506442253176	25.20872010939046	25.437283914933356
36	24.74158727335386	24.39674052004936	25.056196013473702	25.805476193123077
37	25.413716051715895	23.200337954265006	25.21094349271286	26.17500250130624
38	24.533700932709305	23.343968516892154	26.25815703756406	25.864173512834483
39	24.65532000044468	22.94398185719209	26.32597022889731	26.07472791346592
40	24.08213177992952	22.63026247040121	27.434771491779042	25.852834257890233
41	23.32640378864518	22.654275010283147	28.21562371460652	25.803697486465154
42	23.733060598312452	22.11999599791002	28.14158504997054	26.00535835380699
43	22.635376252042732	21.865418607495023	28.853734728135805	26.64547041232644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	9.5
2	14.0
3	35.0
4	56.0
5	56.0
6	65.0
7	74.0
8	83.0
9	92.0
10	137.0
11	182.0
12	182.0
13	294.5
14	407.0
15	750.0
16	1093.0
17	1147.0
18	1201.0
19	1201.0
20	1464.0
21	1727.0
22	2034.0
23	2341.0
24	2879.5
25	3418.0
26	3418.0
27	4128.0
28	4838.0
29	6346.0
30	7854.0
31	9081.5
32	10309.0
33	10309.0
34	11954.0
35	13599.0
36	15220.0
37	16841.0
38	19061.0
39	21281.0
40	21281.0
41	23117.5
42	24954.0
43	25230.5
44	25507.0
45	27509.0
46	29511.0
47	29511.0
48	30820.0
49	32129.0
50	33839.5
51	35550.0
52	37757.0
53	39964.0
54	39964.0
55	37201.5
56	34439.0
57	33123.0
58	31807.0
59	29441.5
60	27076.0
61	27076.0
62	25095.5
63	23115.0
64	20832.5
65	18550.0
66	16082.0
67	13614.0
68	13614.0
69	11683.5
70	9753.0
71	8497.0
72	7241.0
73	5869.5
74	4498.0
75	4498.0
76	3579.5
77	2661.0
78	2226.5
79	1792.0
80	1421.0
81	1050.0
82	1050.0
83	797.5
84	545.0
85	454.5
86	364.0
87	278.5
88	193.0
89	193.0
90	146.0
91	99.0
92	57.0
93	15.0
94	10.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	449765.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.96871289441596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.94312868520629	63.631736606951236
2	5.209535039639993	7.290089230039411
3	1.3100002638899413	2.7497709706717335
4	0.6481597185558681	1.8140360502944395
5	0.36775599121170965	1.2865706682146738
6	0.24848445678718722	1.0431682569400569
7	0.1690919286075604	0.8281801223853832
8	0.11518085914418198	0.6447245171513148
9	0.0968497969549974	0.6098810073324026
>10	0.7508319373500558	10.724762887907733
>50	0.09992807080281674	4.849084929395222
>100	0.041053251849438116	4.527994752716405
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2233833224016988E-4	0.0
10	0.0	0.0	0.0	8.893533289606795E-4	0.0
11	0.0	0.0	0.0	8.893533289606795E-4	0.0
12	0.0	0.0	0.0	8.893533289606795E-4	0.0
13	0.0	0.0	0.0	8.893533289606795E-4	0.0
14	0.0	0.0	0.0	8.893533289606795E-4	0.0
15	0.0	0.0	0.0	8.893533289606795E-4	0.0
16	0.0	0.0	0.0	0.0011116916612008494	0.0
17	0.0	0.0	0.0	0.0013340299934410191	0.0
18	0.0	0.0	0.0	0.001556368325681189	0.0
19	0.0	0.0	0.0	0.001556368325681189	0.0
20	0.0	0.0	0.0	0.002223383322401699	0.0
21	0.0	0.0	0.0	0.0024457216546418686	0.0
22	0.0	0.0	0.0	0.003112736651362378	0.0
23	0.0	0.0	0.0	0.003557413315842718	0.0
24	0.0	0.0	0.0	0.004891443309283737	0.0
25	0.0	0.0	0.0	0.007114826631685436	0.0
26	0.0	0.0	0.0	0.008893533289606795	0.0
27	0.0	0.0	0.0	0.024457216546418685	0.0
28	0.0	0.0	0.0	0.07337164963925606	0.0
29	0.0	0.0	0.0	0.15563683256811892	0.0
30	0.0	0.0	0.0	0.2723644569942081	0.0
31	0.0	0.0	0.0	0.5493980189654597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	265	0.0	25.830189	1
GTATATA	70	1.9175059E-7	23.785715	1
CTAGATA	40	0.0019293694	23.125002	3
GGCCGTT	85	5.1630195E-8	21.764706	7
GGATAAC	60	3.719797E-5	21.583334	1
CCGATCG	45	0.0038222412	20.555557	18
CGTCGTA	45	0.0038222412	20.555557	10
ATACCGT	45	0.0038222412	20.555557	6
TATATAT	55	5.136405E-4	20.181818	2
TGATTCA	110	1.7462298E-9	20.181818	15
CAAGACG	75	9.245219E-6	19.733334	4
CTTCGAC	60	9.2249014E-4	18.5	29
GTATTAA	60	9.2249014E-4	18.5	1
AAGAGCG	50	0.007028387	18.5	5
ATGGCCG	130	6.9303496E-10	18.5	5
CCTAAAC	60	9.2249014E-4	18.5	3
TATTGGG	60	9.2249014E-4	18.5	32
TAACCTT	50	0.007028387	18.5	5
TAACACT	60	9.2249014E-4	18.5	4
CACTAAC	50	0.007028387	18.5	1
>>END_MODULE