FastQCFastQC Report
Fri 10 Feb 2017
ERR1632091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050971
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36540.11976515017677977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31450.10308193686534549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.022.9525431
CTTATAC32150.019.27682537
TAGCGAA803.3848613E-416.187510
TCTTATA56600.016.11395837
GTATCAA21900.015.4589052
CTAGTAC855.3677656E-415.2352933
CGAATAG750.00410647514.830
ATAATAC2550.014.5098043
GTATTAG6250.013.9121
TATACAC10700.013.65887837
ACCGTCG3400.013.0588238
TAATACT3700.013.04
GGACCGT1857.395829E-813.06
CTCTTAT86700.012.82410637
TGTATCG1307.005209E-512.8076932
TAACGCC4550.012.6043974
ATAACGC5450.012.5596333
TACCGTC3400.012.5147067
ATTAGAG3700.012.53
GTCTAGA3001.8189894E-1212.3333331
Produced by FastQC (version 0.11.5)