##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632091.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3050971
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77641216517627	34.0	31.0	34.0	31.0	34.0
2	32.94164415197653	34.0	31.0	34.0	31.0	34.0
3	33.0281674260424	34.0	33.0	34.0	31.0	34.0
4	36.422813917274205	37.0	37.0	37.0	35.0	37.0
5	36.29279629337677	37.0	37.0	37.0	35.0	37.0
6	36.31564082385575	37.0	37.0	37.0	35.0	37.0
7	36.291191558359614	37.0	37.0	37.0	35.0	37.0
8	36.280243568359055	37.0	37.0	37.0	35.0	37.0
9	38.07521080993559	39.0	38.0	39.0	37.0	39.0
10	38.036167502083764	39.0	38.0	39.0	35.0	39.0
11	38.10600035201908	39.0	38.0	39.0	37.0	39.0
12	38.069153721880674	39.0	38.0	39.0	37.0	39.0
13	38.089529857871476	39.0	38.0	39.0	37.0	39.0
14	39.49526921101511	40.0	39.0	41.0	37.0	41.0
15	39.505780618694835	40.0	39.0	41.0	37.0	41.0
16	39.50265046767078	40.0	39.0	41.0	37.0	41.0
17	39.48665490429113	40.0	39.0	41.0	37.0	41.0
18	39.48114813284033	40.0	39.0	41.0	37.0	41.0
19	39.506508255896236	41.0	39.0	41.0	37.0	41.0
20	39.48061420446147	40.0	39.0	41.0	37.0	41.0
21	39.45751008449441	40.0	39.0	41.0	37.0	41.0
22	39.41826454594291	40.0	39.0	41.0	37.0	41.0
23	39.371234928158934	40.0	39.0	41.0	37.0	41.0
24	39.3287258384298	40.0	39.0	41.0	36.0	41.0
25	39.27816029716441	40.0	39.0	41.0	36.0	41.0
26	39.1971028239862	40.0	39.0	41.0	36.0	41.0
27	39.12273141894826	40.0	39.0	41.0	36.0	41.0
28	39.062852121504925	40.0	39.0	41.0	36.0	41.0
29	38.99713664928313	40.0	39.0	41.0	35.0	41.0
30	38.93180957799992	40.0	38.0	41.0	35.0	41.0
31	38.86230252598271	40.0	38.0	41.0	35.0	41.0
32	38.78602320376038	40.0	38.0	41.0	35.0	41.0
33	38.70912440662333	40.0	38.0	41.0	35.0	41.0
34	38.63677432528857	40.0	38.0	41.0	35.0	41.0
35	38.61205957054328	40.0	38.0	41.0	35.0	41.0
36	38.582782661650995	40.0	38.0	41.0	35.0	41.0
37	38.52926002902027	40.0	38.0	41.0	35.0	41.0
38	38.48418028227735	40.0	38.0	41.0	35.0	41.0
39	38.43402280782085	40.0	38.0	41.0	34.0	41.0
40	38.36470225380707	40.0	38.0	41.0	34.0	41.0
41	38.31801449440194	40.0	38.0	41.0	34.0	41.0
42	38.263051992300156	40.0	38.0	41.0	34.0	41.0
43	37.3956156908735	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	3.0
12	2.0
13	1.0
14	2.0
15	1.0
16	11.0
17	17.0
18	52.0
19	66.0
20	160.0
21	326.0
22	595.0
23	1030.0
24	1722.0
25	2684.0
26	4064.0
27	6133.0
28	8928.0
29	12898.0
30	18043.0
31	25261.0
32	34700.0
33	48635.0
34	72664.0
35	108748.0
36	175213.0
37	322665.0
38	785916.0
39	1420424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.178417625077394	17.5212088217161	12.021353201980615	27.279020351225892
2	19.494416695537257	19.413360533417066	33.271342139928564	27.820880631117113
3	20.273807912300708	20.746411552256642	28.744160465635364	30.235620069807283
4	15.379792203859033	14.980673365954642	32.75203205799072	36.887502372195605
5	16.861681084480974	33.82808292835298	32.66818989757687	16.642046089589183
6	36.674029350000374	32.858129428303315	15.09345713217202	15.374384089524288
7	31.233105788288384	28.589291736958494	20.1236589924978	20.05394348225532
8	28.45851369940914	31.804661532344948	19.182057122142425	20.554767646103485
9	27.351521859762023	13.766371427325923	18.705323649421775	40.17678306349028
10	18.11393815280447	25.16851192620317	31.66129733779836	25.056252583194006
11	38.18646588250101	20.16518675529856	20.41399279114747	21.234354571052954
12	23.452894176968577	23.68482034080298	27.656408402439748	25.205877079788696
13	31.92255842484245	18.136357244955786	22.901528726428406	27.03955560377336
14	24.027006484165206	19.10391806411795	23.346731253754953	33.52234419796189
15	26.728375982597015	25.77038588698483	20.716158888432567	26.78507924198558
16	26.835882740281704	24.2387095780327	22.595822772487843	26.329584909197763
17	25.594638559330786	24.431107342547666	23.91677928108789	26.05747481703366
18	25.916339421121997	23.444732840790685	24.6161959585981	26.022731779489217
19	26.376848550838407	23.799406811798605	24.231367653117648	25.59237698424534
20	26.50061242797785	23.52185582884924	23.957946502933	26.019585240239913
21	27.14591518569006	23.57383927936385	23.528673330556074	25.751572204390012
22	27.289344933137684	23.365315501196175	23.362103409045844	25.983236156620304
23	26.53499492456664	23.292486228154903	23.69389941759525	26.478619429683203
24	26.283828984280746	23.64738963431642	23.54165280495947	26.52712857644337
25	26.45610200818035	22.934239624041002	23.859289386886996	26.750368980891658
26	26.12604315150816	23.643817001210437	24.02064785276556	26.209491994515844
27	26.294088013291507	23.32742592440243	23.77466059166082	26.603825470645248
28	26.05124729143607	23.431720589936777	24.13893806266923	26.378094055957924
29	25.711126064456202	23.810747463676318	24.322879502951682	26.155246968915797
30	25.641312224862183	23.994525021706202	24.050343316930906	26.313819436500708
31	26.258918881890388	23.68908127936975	23.622020661618876	26.429979177120988
32	25.40299465317763	23.62467555411048	24.047917859592896	26.924411933118996
33	25.323052890374903	23.264626245218327	24.68820582037653	26.724115044030246
34	26.112244265841923	22.705820540411562	24.69272897054741	26.48920622319911
35	25.65599607469229	22.806837560894547	25.319578586620455	26.217587777792712
36	25.263366973989594	22.789859359528492	25.174411687295617	26.7723619791863
37	25.03661948933635	21.893685649585002	25.972747692455943	27.09694716862271
38	24.433434470534134	21.761367118861504	26.85859682048764	26.94660159011672
39	24.267192313529037	21.28279816491209	27.510159880247958	26.939849641310914
40	23.999802030238897	21.396335789491282	28.135174015092247	26.46868816517758
41	23.186093869787683	21.34199243453969	28.925217578272623	26.546696117400003
42	22.94653079298361	21.593486139330725	29.04730985643587	26.412673211249793
43	22.20483249431083	21.03366436455804	29.383334027101537	27.3781691140296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	145.0
2	149.0
3	371.5
4	594.0
5	594.0
6	753.0
7	912.0
8	872.0
9	832.0
10	1245.0
11	1658.0
12	1658.0
13	2827.0
14	3996.0
15	5874.5
16	7753.0
17	7899.0
18	8045.0
19	8045.0
20	9574.0
21	11103.0
22	11484.0
23	11865.0
24	14865.5
25	17866.0
26	17866.0
27	22097.0
28	26328.0
29	32464.5
30	38601.0
31	46125.5
32	53650.0
33	53650.0
34	64783.5
35	75917.0
36	88418.5
37	100920.0
38	114039.0
39	127158.0
40	127158.0
41	140024.5
42	152891.0
43	165653.5
44	178416.0
45	188964.0
46	199512.0
47	199512.0
48	208996.5
49	218481.0
50	226301.5
51	234122.0
52	238608.5
53	243095.0
54	243095.0
55	239678.5
56	236262.0
57	230490.5
58	224719.0
59	217578.0
60	210437.0
61	210437.0
62	195466.0
63	180495.0
64	163153.5
65	145812.0
66	128768.5
67	111725.0
68	111725.0
69	96707.0
70	81689.0
71	69229.5
72	56770.0
73	45504.5
74	34239.0
75	34239.0
76	27783.0
77	21327.0
78	17554.5
79	13782.0
80	11410.0
81	9038.0
82	9038.0
83	6917.0
84	4796.0
85	3916.0
86	3036.0
87	2411.0
88	1786.0
89	1786.0
90	1321.0
91	856.0
92	504.5
93	153.0
94	95.0
95	37.0
96	37.0
97	21.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3050971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.050025703498335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.301198171689	51.4659031521732
2	8.819165319369388	10.768205388618043
3	2.6753929939353847	4.899984331401438
4	1.2259227978930507	2.9937047328750133
5	0.7012181564374097	2.140469323713179
6	0.4484322570536077	1.6426080491640311
7	0.2891412991656346	1.2356458622203426
8	0.23103006845327922	1.128351329388294
9	0.16900871192423791	0.9286187586380883
>10	0.9998836121803842	11.464644559453612
>50	0.08645829706772659	3.648182938829883
>100	0.04917461290634917	5.656738085902427
>500	0.00338301650350364	1.3966564785079465
>1k	5.906854210644607E-4	0.6302870091145572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3654	0.11976515017677977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3145	0.10308193686534549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.277645051362337E-5	0.0
2	0.0	0.0	0.0	6.555290102724674E-5	0.0
3	0.0	0.0	0.0	6.555290102724674E-5	0.0
4	0.0	0.0	0.0	9.83293515408701E-5	0.0
5	0.0	3.277645051362337E-5	0.0	9.83293515408701E-5	0.0
6	0.0	3.277645051362337E-5	0.0	9.83293515408701E-5	0.0
7	0.0	3.277645051362337E-5	0.0	9.83293515408701E-5	0.0
8	0.0	3.277645051362337E-5	0.0	9.83293515408701E-5	0.0
9	0.0	3.277645051362337E-5	0.0	1.3110580205449347E-4	0.0
10	0.0	3.277645051362337E-5	0.0	1.3110580205449347E-4	0.0
11	0.0	3.277645051362337E-5	0.0	1.966587030817402E-4	0.0
12	0.0	3.277645051362337E-5	0.0	1.966587030817402E-4	0.0
13	0.0	3.277645051362337E-5	0.0	2.6221160410898694E-4	0.0
14	0.0	6.555290102724674E-5	0.0	2.6221160410898694E-4	0.0
15	0.0	1.3110580205449347E-4	0.0	4.2609385667710377E-4	0.0
16	0.0	1.3110580205449347E-4	0.0	5.244232082179739E-4	6.555290102724674E-5
17	0.0	1.3110580205449347E-4	0.0	7.210819112997141E-4	6.555290102724674E-5
18	0.0	1.3110580205449347E-4	0.0	7.538583618133375E-4	6.555290102724674E-5
19	0.0	1.3110580205449347E-4	0.0	9.505170648950777E-4	6.555290102724674E-5
20	0.0	1.3110580205449347E-4	0.0	0.0010816228669495711	6.555290102724674E-5
21	0.0	1.3110580205449347E-4	0.0	0.0014421638225994282	6.555290102724674E-5
22	0.0	1.3110580205449347E-4	0.0	0.001704375426708415	6.555290102724674E-5
23	0.0	1.3110580205449347E-4	0.0	0.002753221843144363	6.555290102724674E-5
24	0.0	1.6388225256811683E-4	0.0	0.004654255972934518	6.555290102724674E-5
25	0.0	1.6388225256811683E-4	0.0	0.006161972696561193	6.555290102724674E-5
26	0.0	1.6388225256811683E-4	0.0	0.01002959385716875	6.555290102724674E-5
27	0.0	1.6388225256811683E-4	0.0	0.03405473208365468	6.555290102724674E-5
28	0.0	1.6388225256811683E-4	0.0	0.1290736621226488	9.83293515408701E-5
29	0.0	2.6221160410898694E-4	0.0	0.2540174914805811	9.83293515408701E-5
30	0.0	2.6221160410898694E-4	0.0	0.4216690358577646	9.83293515408701E-5
31	0.0	2.6221160410898694E-4	0.0	0.9918153925422432	9.83293515408701E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1475	0.0	22.952543	1
CTTATAC	3215	0.0	19.276825	37
TAGCGAA	80	3.3848613E-4	16.1875	10
TCTTATA	5660	0.0	16.113958	37
GTATCAA	2190	0.0	15.458905	2
CTAGTAC	85	5.3677656E-4	15.235293	3
CGAATAG	75	0.004106475	14.8	30
ATAATAC	255	0.0	14.509804	3
GTATTAG	625	0.0	13.912	1
TATACAC	1070	0.0	13.658878	37
ACCGTCG	340	0.0	13.058823	8
TAATACT	370	0.0	13.0	4
GGACCGT	185	7.395829E-8	13.0	6
CTCTTAT	8670	0.0	12.824106	37
TGTATCG	130	7.005209E-5	12.807693	2
TAACGCC	455	0.0	12.604397	4
ATAACGC	545	0.0	12.559633	3
TACCGTC	340	0.0	12.514706	7
ATTAGAG	370	0.0	12.5	3
GTCTAGA	300	1.8189894E-12	12.333333	1
>>END_MODULE