##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632090.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306803
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75698086394201	34.0	31.0	34.0	31.0	34.0
2	32.927138913244	34.0	31.0	34.0	31.0	34.0
3	33.00993145438604	34.0	33.0	34.0	31.0	34.0
4	36.40624765729149	37.0	37.0	37.0	35.0	37.0
5	36.27645427195953	37.0	37.0	37.0	35.0	37.0
6	36.3106781876318	37.0	37.0	37.0	35.0	37.0
7	36.286111935020195	37.0	37.0	37.0	35.0	37.0
8	36.2736479108744	37.0	37.0	37.0	35.0	37.0
9	38.067698164620296	39.0	38.0	39.0	37.0	39.0
10	38.02575594110879	39.0	38.0	39.0	35.0	39.0
11	38.10145598315531	39.0	38.0	39.0	37.0	39.0
12	38.0726752997852	39.0	38.0	39.0	37.0	39.0
13	38.08416801661001	39.0	38.0	39.0	37.0	39.0
14	39.482778851575766	40.0	39.0	41.0	37.0	41.0
15	39.50178127332523	40.0	39.0	41.0	37.0	41.0
16	39.489196650619455	40.0	39.0	41.0	37.0	41.0
17	39.481784728311	40.0	39.0	41.0	37.0	41.0
18	39.46900779979335	40.0	39.0	41.0	37.0	41.0
19	39.502400563227866	41.0	39.0	41.0	37.0	41.0
20	39.47316356098213	40.0	39.0	41.0	37.0	41.0
21	39.44665469372855	40.0	39.0	41.0	37.0	41.0
22	39.410142664837046	40.0	39.0	41.0	37.0	41.0
23	39.374377043249254	40.0	39.0	41.0	37.0	41.0
24	39.331186461670846	40.0	39.0	41.0	36.0	41.0
25	39.27491908488509	40.0	39.0	41.0	36.0	41.0
26	39.19724057457065	40.0	39.0	41.0	36.0	41.0
27	39.123098535542354	40.0	39.0	41.0	36.0	41.0
28	39.06432466436117	40.0	39.0	41.0	36.0	41.0
29	39.01178606467342	40.0	39.0	41.0	35.0	41.0
30	38.954087802270514	40.0	39.0	41.0	35.0	41.0
31	38.88478926216497	40.0	38.0	41.0	35.0	41.0
32	38.801419803587315	40.0	38.0	41.0	35.0	41.0
33	38.73605212465328	40.0	38.0	41.0	35.0	41.0
34	38.67084415732571	40.0	38.0	41.0	35.0	41.0
35	38.64493176403099	40.0	38.0	41.0	35.0	41.0
36	38.61816540255473	40.0	38.0	41.0	35.0	41.0
37	38.56669589280418	40.0	38.0	41.0	35.0	41.0
38	38.51883782101218	40.0	38.0	41.0	35.0	41.0
39	38.4763056423829	40.0	38.0	41.0	35.0	41.0
40	38.426227905203014	40.0	38.0	41.0	34.0	41.0
41	38.38545255424491	40.0	38.0	41.0	34.0	41.0
42	38.345524652627255	40.0	38.0	41.0	34.0	41.0
43	37.47101886226667	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	0.0
18	7.0
19	12.0
20	21.0
21	33.0
22	67.0
23	107.0
24	203.0
25	264.0
26	416.0
27	593.0
28	890.0
29	1307.0
30	1787.0
31	2552.0
32	3462.0
33	4904.0
34	7142.0
35	10850.0
36	17418.0
37	31858.0
38	79182.0
39	143726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62181269413924	17.889655577031515	12.615587200907422	26.872944527921828
2	19.9065850073174	20.143870822645148	33.411016189541826	26.53852798049563
3	20.548364911686	21.003054077046183	29.695928657803215	28.752652353464605
4	16.106100657425124	15.358389585499491	32.63983728972663	35.89567246734875
5	16.49038633911663	34.269221617780794	33.074969931845516	16.16542211125706
6	36.227155536288755	32.76108773382268	15.272340883237778	15.73941584665078
7	30.335426967793666	28.881725406857168	20.350192142840847	20.43265548250832
8	27.505923996831843	31.988931007845423	19.907562833479464	20.597582161843267
9	26.922161778079097	14.06342180487153	19.10835291701841	39.90606350003097
10	17.835223254009904	25.858938797860514	32.27576001538446	24.030077932745115
11	37.15934981079064	20.49784389331265	20.529460272552747	21.813346023343968
12	22.650691160125554	24.224991281050055	27.788515757668602	25.33580180115579
13	31.672767215444438	18.596297950150422	23.303227152276868	26.427707682128272
14	24.173818378568658	19.912126022235768	23.089735106892697	32.824320492302874
15	26.73311538674654	26.268647959765712	21.0108766863427	25.98735996714504
16	26.610235232380386	24.88502394044387	22.419272301770192	26.085468525405552
17	25.442384852820865	25.030068154483498	23.734774431801515	25.79277256089412
18	24.920225682278204	23.72760370661304	25.306141074239825	26.046029536868936
19	26.12914476064445	24.292461286232534	24.56690449571875	25.011489457404263
20	26.602086681029846	23.42252194404879	24.58222377225777	25.393167602663596
21	26.46682072861087	24.13437939003204	23.431974263615416	25.96682561774168
22	26.835461191709335	23.89318227005603	23.42154411788672	25.849812420347913
23	26.084816641297508	23.974667783561436	23.565936447818306	26.374579127322743
24	26.525490298334763	24.177729683216917	23.60830891484112	25.688471103607203
25	26.459650003422393	23.448597308370516	23.850157918925174	26.241594769281917
26	26.083838815135447	23.963911695778727	23.713262256236085	26.23898723284974
27	26.140226790481186	23.583211376681454	23.890248791569835	26.386313041267524
28	25.46715644892651	23.926428359566238	24.36384259606327	26.24257259544398
29	25.36415875985567	24.071798515659886	24.627203775712754	25.93683894877169
30	25.171527005928883	24.852429735041703	24.178381567324962	25.797661691704448
31	26.220082593716487	24.019321844962402	23.93718444734895	25.823411113972156
32	25.062662359885657	24.051264166256523	24.37981375671033	26.506259717147486
33	25.174786426469105	23.595271232680254	24.727593928351418	26.502348412499227
34	25.798639517866512	23.184258302558973	24.65393102414253	26.363171155431985
35	25.499424712274653	23.356681649136416	25.080589172856847	26.063304465732084
36	24.977917425840033	23.47858397734051	25.25659788202853	26.286900714790924
37	25.465200796602378	22.26999084102828	25.589384719184622	26.675423643184715
38	24.35797563909088	22.505321004031902	26.901953370729753	26.234749986147467
39	24.32994462244502	21.915039944198718	27.490278778238803	26.264736655117453
40	23.78399168195878	21.764454715240724	28.18127593276467	26.270277670035817
41	23.032695247438912	21.945352555222733	28.957343963390187	26.064608233948167
42	23.177739461478538	21.724363842596066	28.733421772277325	26.364474923648075
43	22.16992663044364	21.712955870705304	29.068490203811564	27.04862729503949
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	10.0
2	10.0
3	24.5
4	39.0
5	39.0
6	47.0
7	55.0
8	59.0
9	63.0
10	89.0
11	115.0
12	115.0
13	175.5
14	236.0
15	354.0
16	472.0
17	523.0
18	574.0
19	574.0
20	750.5
21	927.0
22	1154.0
23	1381.0
24	1770.5
25	2160.0
26	2160.0
27	2510.0
28	2860.0
29	3921.0
30	4982.0
31	5720.0
32	6458.0
33	6458.0
34	7526.0
35	8594.0
36	9660.5
37	10727.0
38	12215.0
39	13703.0
40	13703.0
41	15117.5
42	16532.0
43	16893.5
44	17255.0
45	18421.0
46	19587.0
47	19587.0
48	20762.5
49	21938.0
50	23076.0
51	24214.0
52	25916.5
53	27619.0
54	27619.0
55	25636.5
56	23654.0
57	23032.0
58	22410.0
59	21143.0
60	19876.0
61	19876.0
62	18368.0
63	16860.0
64	14979.0
65	13098.0
66	11491.0
67	9884.0
68	9884.0
69	8473.5
70	7063.0
71	6146.5
72	5230.0
73	4223.5
74	3217.0
75	3217.0
76	2608.0
77	1999.0
78	1692.0
79	1385.0
80	1069.5
81	754.0
82	754.0
83	581.0
84	408.0
85	329.0
86	250.0
87	189.0
88	128.0
89	128.0
90	93.0
91	58.0
92	36.5
93	15.0
94	7.5
95	0.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	306803.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.07847070834175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.87032003956116	68.97483132056173
2	4.668533681855102	7.010127385681302
3	1.1877600928576777	2.6752563402045726
4	0.5758576474893016	1.7293804607680048
5	0.32028659384463354	1.2023313827119435
6	0.2075798919587455	0.9350868502839252
7	0.15963618385398345	0.8389668387430927
8	0.13057065825413	0.7842436272881279
9	0.09818331075727782	0.6634307538664469
>10	0.7074428332223179	10.616978618810998
>50	0.058194675825434534	2.9501660752057597
>100	0.01563439052026049	1.6192003458739854
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.2594205402163603E-4	0.0
16	0.0	0.0	0.0	3.2594205402163603E-4	0.0
17	0.0	0.0	0.0	3.2594205402163603E-4	0.0
18	0.0	0.0	0.0	6.518841080432721E-4	0.0
19	0.0	0.0	0.0	6.518841080432721E-4	0.0
20	0.0	0.0	0.0	0.0013037682160865441	0.0
21	0.0	0.0	0.0	0.0019556523241298163	0.0
22	0.0	0.0	0.0	0.00325942054021636	0.0
23	0.0	0.0	0.0	0.00488913081032454	0.0
24	0.0	0.0	0.0	0.007170725188475993	0.0
25	0.0	0.0	0.0	0.009452319566627445	0.0
26	0.0	0.0	0.0	0.014993334484995258	0.0
27	3.2594205402163603E-4	0.0	0.0	0.03454985772629342	0.0
28	3.2594205402163603E-4	0.0	0.0	0.1059311675570317	0.0
29	3.2594205402163603E-4	0.0	0.0	0.2144698715462365	0.0
30	3.2594205402163603E-4	0.0	0.0	0.3588622014778213	0.0
31	3.2594205402163603E-4	0.0	0.0	0.7395625205750922	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTAGA	25	0.0054901424	29.6	1
GCGATTT	25	0.0054901424	29.6	27
ACCTTAA	25	0.0054901424	29.6	8
GGTATCA	110	0.0	26.90909	1
TGACGGG	50	2.6958503E-4	22.2	20
TATCACT	45	0.003819514	20.555555	4
CTAAGTA	45	0.003819514	20.555555	18
AGTACGC	45	0.003819514	20.555555	21
GACCACG	65	6.881074E-5	19.923077	11
AGGATTG	65	6.881074E-5	19.923077	5
CTTATAC	285	0.0	18.82456	37
AAGTACG	60	9.215794E-4	18.5	20
CACGGGT	60	9.215794E-4	18.5	14
ATGTACA	50	0.0070234193	18.5	4
TTATAAC	50	0.0070234193	18.5	2
GTACGCA	50	0.0070234193	18.5	22
TAGAACT	65	0.0015763678	17.076923	4
CTTAGGA	130	1.38152245E-8	17.076923	2
AAATGTC	65	0.0015763678	17.076923	7
TTAGGAC	110	7.761828E-7	16.818182	3
>>END_MODULE