FastQCFastQC Report
Fri 10 Feb 2017
ERR1632089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036296
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.23410299759914155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18920.18257331882010544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18450.17803793510734384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.026.2233031
CCGTATA400.001931059823.1252
GCGGTAA500.00703442718.49999834
CGCTCTA500.00703442718.49999814
CCCGTAT500.00703442718.4999981
GTGCTAT1054.7961294E-717.6190471
GTATTAT953.6056936E-617.5263161
GCTTATA953.6056936E-617.5263161
CTTATAC11850.016.54852337
GTATATA1504.667527E-916.0333331
TACTAGG700.00259226215.8571432
GTCCAAT1101.4515146E-515.1363641
GTATCAA8700.015.0977022
TCGTCTA750.00410404314.829
TAATACT1001.0931989E-414.7999994
ATCAACG14350.014.3101032
TCAACGC14500.014.1620693
CGGAGTG1458.906736E-714.03448325
TATCAAC14650.014.0170651
CAACGCA14950.013.9832774
Produced by FastQC (version 0.11.5)