##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1036296
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86939252877556	34.0	31.0	34.0	31.0	34.0
2	33.022167411627564	34.0	33.0	34.0	31.0	34.0
3	33.11090460640589	34.0	33.0	34.0	31.0	34.0
4	36.47368994958969	37.0	37.0	37.0	35.0	37.0
5	36.35124423909771	37.0	37.0	37.0	35.0	37.0
6	36.374898677597905	37.0	37.0	37.0	35.0	37.0
7	36.35886368373515	37.0	37.0	37.0	35.0	37.0
8	36.34886364513614	37.0	37.0	37.0	35.0	37.0
9	38.153467735087276	39.0	39.0	39.0	37.0	39.0
10	38.128925519349686	39.0	38.0	39.0	37.0	39.0
11	38.19426592402171	39.0	39.0	39.0	37.0	39.0
12	38.16470004709079	39.0	39.0	39.0	37.0	39.0
13	38.183565313385365	39.0	39.0	39.0	37.0	39.0
14	39.619646317268426	41.0	39.0	41.0	37.0	41.0
15	39.62285196507562	41.0	39.0	41.0	37.0	41.0
16	39.62193137867945	41.0	39.0	41.0	37.0	41.0
17	39.60910010267337	41.0	39.0	41.0	37.0	41.0
18	39.603397098898384	41.0	39.0	41.0	37.0	41.0
19	39.62358631124698	41.0	39.0	41.0	37.0	41.0
20	39.59921296617955	41.0	39.0	41.0	37.0	41.0
21	39.582006492353536	41.0	39.0	41.0	37.0	41.0
22	39.54329747485274	41.0	39.0	41.0	37.0	41.0
23	39.500072373144356	41.0	39.0	41.0	37.0	41.0
24	39.46162486393849	41.0	39.0	41.0	37.0	41.0
25	39.41731995491636	41.0	39.0	41.0	37.0	41.0
26	39.33725402780673	41.0	39.0	41.0	36.0	41.0
27	39.27125647498398	40.0	39.0	41.0	36.0	41.0
28	39.22747168762593	40.0	39.0	41.0	36.0	41.0
29	39.173602908821415	40.0	39.0	41.0	36.0	41.0
30	39.11510514370411	40.0	39.0	41.0	36.0	41.0
31	39.04842342342342	40.0	39.0	41.0	35.0	41.0
32	38.9800983502783	40.0	39.0	41.0	35.0	41.0
33	38.90858692883114	40.0	38.0	41.0	35.0	41.0
34	38.83225159606908	40.0	38.0	41.0	35.0	41.0
35	38.81965094914966	40.0	38.0	41.0	35.0	41.0
36	38.78597041771849	40.0	38.0	41.0	35.0	41.0
37	38.73429888757652	40.0	38.0	41.0	35.0	41.0
38	38.69502825447555	40.0	38.0	41.0	35.0	41.0
39	38.64342620255216	40.0	38.0	41.0	35.0	41.0
40	38.580356384662295	40.0	38.0	41.0	35.0	41.0
41	38.54014972556104	40.0	38.0	41.0	35.0	41.0
42	38.49057508665478	40.0	38.0	41.0	35.0	41.0
43	37.66678149872237	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	0.0
13	1.0
14	1.0
15	1.0
16	3.0
17	3.0
18	14.0
19	22.0
20	45.0
21	66.0
22	172.0
23	274.0
24	455.0
25	777.0
26	1150.0
27	1705.0
28	2645.0
29	3753.0
30	5428.0
31	7630.0
32	10526.0
33	14759.0
34	21948.0
35	33490.0
36	52608.0
37	100144.0
38	249045.0
39	529628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03577356276585	17.6750658113126	12.14440661741433	27.14475400850722
2	18.514690783328316	19.566706809637402	34.619452357241556	27.299150049792726
3	19.70730370473301	21.250878127484814	29.28400765804365	29.75781050973853
4	14.781780495148105	14.945729791488146	33.86484170545867	36.407648007905074
5	16.07851424689471	34.67706138014621	33.177103839057565	16.067320533901512
6	35.5718829369215	33.85355149493967	15.517381134347715	15.057184433791118
7	30.749805074997877	29.57851810679574	20.31765055543976	19.35402626276662
8	28.190208203061673	32.985170260234526	19.297768205223218	19.52685333148058
9	27.387445285902867	13.980175548298943	18.83757150466662	39.79480766113157
10	17.266302292009232	26.110976014574987	32.54601002030308	24.0767116731127
11	37.06624362151354	20.683762168338006	21.10941275465697	21.140581455491482
12	22.6734446528791	24.112512255185777	29.04604475941237	24.167998332522757
13	31.524776704725294	18.717335587515535	23.60464577688228	26.15324193087689
14	23.30096806317886	19.071770999791564	24.842902027992004	32.78435890903757
15	25.75297019384423	26.419575102094385	21.84945228004354	25.97800242401785
16	25.77014675343724	25.122262365192956	23.4312397230135	25.676351158356297
17	24.656661803191366	25.4126234203355	24.832094305102018	25.098620471371113
18	25.052012166408055	24.443305773639963	25.502173124281093	25.002508935670892
19	25.449678470244024	24.486633162725706	25.416193828790227	24.64749453824004
20	25.696712136300825	24.370160649080958	25.105664790754766	24.82746242386345
21	25.634664227209214	24.351247134023485	24.829392374379523	25.184696264387778
22	25.912577101523116	24.301068420605695	24.64276615947567	25.143588318395516
23	25.736758609509252	24.149856797671706	24.78645097539699	25.32693361742205
24	25.399885746929453	24.315929039579427	24.759335170646224	25.5248500428449
25	25.410210982190417	23.788763056115243	25.06822375074303	25.732802210951313
26	25.076426040436324	24.29730501709936	25.22184781182211	25.404421130642213
27	25.322687726286695	24.240467974401135	24.92897782100867	25.507866478303498
28	25.257165906266167	24.42941012992427	25.06851324332044	25.244910720489127
29	25.069671213630084	24.609667508125092	25.192705559029466	25.12795571921536
30	24.916433142654224	24.604070651628493	24.931776249256966	25.54771995646032
31	25.28293074565568	24.4106896099184	24.77043238611362	25.535947258312298
32	24.732219365895457	24.29141866802535	25.244814222963324	25.73154774311587
33	24.812022819734903	24.182086971290058	25.597319684723285	25.408570524251754
34	25.13664049653767	23.614488524514233	25.6624555146406	25.586415464307493
35	24.790986359109752	23.80082524684067	26.069675073531112	25.338513320518462
36	24.653863374943068	23.61024263337888	25.919814415958374	25.81607957571968
37	24.335421539791717	22.98802658699831	26.38560797301157	26.2909439001984
38	24.015628739279123	23.089541984143526	26.985919081034766	25.908910195542585
39	23.674123995460754	22.72593930691617	27.587677651945004	26.01225904567807
40	23.481997421586108	22.57858758501432	28.138003041602012	25.801411951797554
41	23.02730108000031	22.493573264781492	28.661502119085668	25.817623536132533
42	22.623941422141936	22.659742004215012	28.784729459536656	25.931587114106396
43	22.27799779213661	22.140681812918316	29.13520847325475	26.44611192169033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	176.0
2	173.0
3	362.0
4	551.0
5	551.0
6	688.5
7	826.0
8	739.0
9	652.0
10	920.5
11	1189.0
12	1189.0
13	1933.5
14	2678.0
15	3808.5
16	4939.0
17	4861.0
18	4783.0
19	4783.0
20	5524.0
21	6265.0
22	6365.5
23	6466.0
24	7864.0
25	9262.0
26	9262.0
27	11215.5
28	13169.0
29	15820.0
30	18471.0
31	21378.0
32	24285.0
33	24285.0
34	27975.5
35	31666.0
36	35489.5
37	39313.0
38	43092.0
39	46871.0
40	46871.0
41	50656.0
42	54441.0
43	57762.0
44	61083.0
45	63809.5
46	66536.0
47	66536.0
48	68845.0
49	71154.0
50	73275.0
51	75396.0
52	76118.5
53	76841.0
54	76841.0
55	75979.0
56	75117.0
57	74623.5
58	74130.0
59	71286.5
60	68443.0
61	68443.0
62	63171.0
63	57899.0
64	51515.0
65	45131.0
66	39097.0
67	33063.0
68	33063.0
69	28497.0
70	23931.0
71	20135.5
72	16340.0
73	13295.0
74	10250.0
75	10250.0
76	8384.0
77	6518.0
78	5098.0
79	3678.0
80	2945.0
81	2212.0
82	2212.0
83	1709.0
84	1206.0
85	937.0
86	668.0
87	492.5
88	317.0
89	317.0
90	236.5
91	156.0
92	95.0
93	34.0
94	21.5
95	9.0
96	9.0
97	6.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1036296.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.69320988344394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07514863385738	66.66690711058088
2	7.259530066555396	10.989942659658986
3	1.965541896828864	4.463345259941091
4	0.8705387620693592	2.635754929159579
5	0.49034807083449383	1.855800972080859
6	0.3209859456185352	1.4577873930803704
7	0.20944167668331637	1.1097318954071471
8	0.13821020269726056	0.8369259104637659
9	0.09946568281478713	0.677598912494977
>10	0.5458656354655944	7.079231500343211
>50	0.0191771577599357	0.9567174155039527
>100	0.005235490374658305	0.5910532805926069
>500	1.2769461010629074E-4	0.08351091294128644
>1k	3.830838303188722E-4	0.5956918477513291
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2426	0.23410299759914155	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1892	0.18257331882010544	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1845	0.17803793510734384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.64975258034384E-5	0.0
5	0.0	0.0	0.0	9.64975258034384E-5	0.0
6	0.0	0.0	0.0	9.64975258034384E-5	0.0
7	0.0	0.0	0.0	9.64975258034384E-5	0.0
8	0.0	0.0	0.0	9.64975258034384E-5	0.0
9	0.0	0.0	0.0	1.929950516068768E-4	0.0
10	0.0	0.0	0.0	1.929950516068768E-4	0.0
11	0.0	0.0	0.0	2.894925774103152E-4	0.0
12	0.0	0.0	0.0	3.859901032137536E-4	0.0
13	0.0	0.0	0.0	4.82487629017192E-4	0.0
14	0.0	0.0	0.0	4.82487629017192E-4	0.0
15	0.0	0.0	0.0	4.82487629017192E-4	0.0
16	0.0	0.0	0.0	4.82487629017192E-4	0.0
17	0.0	9.64975258034384E-5	0.0	6.754826806240688E-4	0.0
18	0.0	9.64975258034384E-5	0.0	6.754826806240688E-4	0.0
19	0.0	9.64975258034384E-5	0.0	8.684777322309456E-4	0.0
20	0.0	9.64975258034384E-5	0.0	9.64975258034384E-4	0.0
21	0.0	9.64975258034384E-5	0.0	0.0010614727838378225	0.0
22	0.0	9.64975258034384E-5	0.0	0.002315940619282522	0.0
23	0.0	9.64975258034384E-5	0.0	0.0030879208257100286	0.0
24	0.0	9.64975258034384E-5	0.0	0.004149393609547851	0.0
25	0.0	9.64975258034384E-5	0.0	0.0056933540224028654	0.0
26	0.0	9.64975258034384E-5	0.0	0.007912797115881949	0.0
27	0.0	9.64975258034384E-5	0.0	0.03271266124736562	0.0
28	0.0	9.64975258034384E-5	0.0	0.1351930336506172	0.0
29	0.0	9.64975258034384E-5	0.0	0.2693245945173966	0.0
30	0.0	9.64975258034384E-5	0.0	0.43530033889931063	0.0
31	0.0	9.64975258034384E-5	0.0	0.9241568046195295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	515	0.0	26.223303	1
CCGTATA	40	0.0019310598	23.125	2
GCGGTAA	50	0.007034427	18.499998	34
CGCTCTA	50	0.007034427	18.499998	14
CCCGTAT	50	0.007034427	18.499998	1
GTGCTAT	105	4.7961294E-7	17.619047	1
GTATTAT	95	3.6056936E-6	17.526316	1
GCTTATA	95	3.6056936E-6	17.526316	1
CTTATAC	1185	0.0	16.548523	37
GTATATA	150	4.667527E-9	16.033333	1
TACTAGG	70	0.002592262	15.857143	2
GTCCAAT	110	1.4515146E-5	15.136364	1
GTATCAA	870	0.0	15.097702	2
TCGTCTA	75	0.004104043	14.8	29
TAATACT	100	1.0931989E-4	14.799999	4
ATCAACG	1435	0.0	14.310103	2
TCAACGC	1450	0.0	14.162069	3
CGGAGTG	145	8.906736E-7	14.034483	25
TATCAAC	1465	0.0	14.017065	1
CAACGCA	1495	0.0	13.983277	4
>>END_MODULE