##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1106859
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.773865505904546	34.0	31.0	34.0	31.0	34.0
2	32.93637129932539	34.0	31.0	34.0	31.0	34.0
3	32.94456385140293	34.0	33.0	34.0	31.0	34.0
4	36.38305240324197	37.0	37.0	37.0	35.0	37.0
5	36.29996322928214	37.0	37.0	37.0	35.0	37.0
6	36.33161405382258	37.0	37.0	37.0	35.0	37.0
7	36.32356424802075	37.0	37.0	37.0	35.0	37.0
8	36.301314801614296	37.0	37.0	37.0	35.0	37.0
9	38.09822118264386	39.0	39.0	39.0	37.0	39.0
10	38.022223246140655	39.0	38.0	39.0	35.0	39.0
11	38.1282701771409	39.0	39.0	39.0	37.0	39.0
12	38.09878945737442	39.0	38.0	39.0	37.0	39.0
13	38.12565376439095	39.0	39.0	39.0	37.0	39.0
14	39.526205234813105	41.0	39.0	41.0	37.0	41.0
15	39.54856580648484	41.0	39.0	41.0	37.0	41.0
16	39.436007657705275	41.0	39.0	41.0	37.0	41.0
17	39.48733488185939	41.0	39.0	41.0	37.0	41.0
18	39.50732116737543	41.0	39.0	41.0	37.0	41.0
19	39.553412855657314	41.0	39.0	41.0	37.0	41.0
20	39.52905744995523	41.0	39.0	41.0	37.0	41.0
21	39.4954018533526	41.0	39.0	41.0	37.0	41.0
22	39.484089662730305	41.0	39.0	41.0	37.0	41.0
23	39.438737002635385	41.0	39.0	41.0	37.0	41.0
24	39.38759950454394	41.0	39.0	41.0	37.0	41.0
25	39.32791981634517	40.0	39.0	41.0	36.0	41.0
26	39.25127229394169	40.0	39.0	41.0	36.0	41.0
27	39.18499104221947	40.0	39.0	41.0	36.0	41.0
28	39.110597646131986	40.0	39.0	41.0	36.0	41.0
29	38.975597614510974	40.0	39.0	41.0	35.0	41.0
30	38.94108915408376	40.0	39.0	41.0	35.0	41.0
31	38.881727482904324	40.0	38.0	41.0	35.0	41.0
32	38.81839330935557	40.0	38.0	41.0	35.0	41.0
33	38.668137495381075	40.0	38.0	41.0	35.0	41.0
34	38.67705823415629	40.0	38.0	41.0	35.0	41.0
35	38.65417004333885	40.0	38.0	41.0	35.0	41.0
36	38.62947493763885	40.0	38.0	41.0	35.0	41.0
37	38.59801925990573	40.0	38.0	41.0	35.0	41.0
38	38.53176330499188	40.0	38.0	41.0	35.0	41.0
39	38.3500834342947	40.0	38.0	41.0	34.0	41.0
40	38.35165725715741	40.0	38.0	41.0	34.0	41.0
41	38.35970435258692	40.0	38.0	41.0	34.0	41.0
42	38.21563993245752	40.0	38.0	41.0	34.0	41.0
43	37.36481069404504	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	4.0
17	3.0
18	18.0
19	30.0
20	76.0
21	110.0
22	229.0
23	372.0
24	666.0
25	1039.0
26	1529.0
27	2188.0
28	3221.0
29	4669.0
30	6444.0
31	9044.0
32	12460.0
33	17466.0
34	25977.0
35	39402.0
36	63779.0
37	117103.0
38	274040.0
39	526988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.154277103045644	17.629887817689514	11.78415678961819	27.431678289646648
2	19.93144564935552	19.586144215297523	33.08659910611921	27.39581102922775
3	20.677882187342743	20.588168863423434	28.436684347328793	30.297264601905034
4	15.386783682474462	14.770354670287725	32.80435900146269	37.03850264577512
5	16.762026599593984	34.09314104145153	32.62104748662657	16.523784872327912
6	37.01374791188399	33.03067509050385	14.6885917718517	15.266985225760463
7	31.623540125707066	28.364407752026228	19.911569585647314	20.10048253661939
8	28.457102485501768	31.74758483239509	19.0610547504244	20.73425793167874
9	27.616525682132952	13.418601646641532	18.44733611056151	40.517536560664006
10	18.12525353274446	25.224622106338746	31.32919369133738	25.32093066957941
11	38.53589300895597	20.12713453113721	19.9317166865879	21.405255773318917
12	23.89879831125735	23.52079171782494	27.27014009914542	25.310269871772284
13	32.49501517356773	17.843736194040975	22.820521855087232	26.840726777304063
14	24.311497670434985	18.9806470381503	23.062016029141923	33.645839262272794
15	27.13272422232642	25.772569044476306	20.30159216304877	26.793114570148504
16	27.197953849586987	24.040008709329733	22.024214466341242	26.73782297474204
17	25.83942489513118	24.370583787094834	23.229607384499744	26.560383933274245
18	26.362346062145225	22.93426714694464	24.098914134501324	26.604472656408813
19	26.694457017560502	23.47263743620461	23.610685733232508	26.22221981300238
20	26.907130899238297	23.226174246222868	23.64474607876884	26.22194877576999
21	27.490131986097598	23.241894405701178	23.397650468578203	25.870323139623025
22	27.547772570851393	23.062829140839078	22.868314753731052	26.521083534578477
23	26.674671299596426	23.039339247365746	23.327180788158202	26.95880866487963
24	26.390443588569095	23.39466905902197	23.12155387452241	27.09333347788652
25	27.014280951774346	22.964171588251077	23.26601671938341	26.755530740591166
26	26.71984417166053	23.29872187875782	23.349496186957868	26.631937762623785
27	26.5147593324895	23.107640629926664	23.50579432429966	26.871805713284168
28	26.143980398587352	22.990010471071745	23.925088922798658	26.940920207542245
29	25.851169841867844	23.4863699893121	23.78713097151489	26.875329197305163
30	25.495568992979234	23.89229341768012	23.99194477345353	26.620192815887116
31	26.742249916204326	23.550876850619638	23.191933209198282	26.51494002397776
32	25.863547208813408	23.33684778277992	23.57626400471966	27.223341003687008
33	25.66984593340254	23.068430576975025	24.035852805099836	27.2258706845226
34	26.468954040216502	22.60829970213008	24.124481980089605	26.79826427756381
35	25.86408928327818	22.884305950441746	24.830805007683907	26.420799758596168
36	25.743839097843534	22.686629462289236	24.66547229592929	26.904059143937936
37	25.80211210280623	22.095406912714267	25.134637745187057	26.96784323929245
38	25.00968958105775	22.007500503677523	26.053725000203276	26.929084915061445
39	25.135450856884212	21.757875212651296	26.312384865642326	26.79428906482217
40	24.573771365639164	22.039663588587164	26.88002717599983	26.506537869773837
41	24.290898840773757	21.554597288362835	27.5606016665176	26.59390220434581
42	24.148333256539452	21.77820300508014	27.684556027461493	26.388907710918915
43	22.99136565723367	21.27560963049494	28.12101631734485	27.612008394926548
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	18.0
2	25.0
3	71.5
4	118.0
5	118.0
6	129.5
7	141.0
8	139.5
9	138.0
10	216.0
11	294.0
12	294.0
13	525.5
14	757.0
15	1148.0
16	1539.0
17	1591.5
18	1644.0
19	1644.0
20	2097.5
21	2551.0
22	2874.5
23	3198.0
24	4067.0
25	4936.0
26	4936.0
27	6323.0
28	7710.0
29	9698.5
30	11687.0
31	14493.0
32	17299.0
33	17299.0
34	21322.5
35	25346.0
36	30298.5
37	35251.0
38	39353.0
39	43455.0
40	43455.0
41	48578.0
42	53701.0
43	58502.5
44	63304.0
45	68525.5
46	73747.0
47	73747.0
48	76784.5
49	79822.0
50	83081.0
51	86340.0
52	88488.5
53	90637.0
54	90637.0
55	89573.0
56	88509.0
57	86521.0
58	84533.0
59	82594.5
60	80656.0
61	80656.0
62	75323.5
63	69991.0
64	63547.0
65	57103.0
66	50379.0
67	43655.0
68	43655.0
69	36787.5
70	29920.0
71	25484.0
72	21048.0
73	16110.0
74	11172.0
75	11172.0
76	8726.5
77	6281.0
78	5150.0
79	4019.0
80	3372.0
81	2725.0
82	2725.0
83	2138.0
84	1551.0
85	1303.0
86	1055.0
87	807.5
88	560.0
89	560.0
90	446.0
91	332.0
92	195.5
93	59.0
94	40.5
95	22.0
96	22.0
97	18.5
98	15.0
99	8.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1106859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.311622624934856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01052796197108	51.014239762518365
2	7.136825360688589	8.465933850664523
3	2.315006290752885	4.11920338474456
4	1.141739565641348	2.7087370501310666
5	0.6919850010099612	2.052137662100899
6	0.46765342866334403	1.6642370208082296
7	0.3191982646640452	1.325251691140155
8	0.2652052101616536	1.258380107461963
9	0.20231995699807126	1.0799932445066385
>10	1.2695403156313156	14.492351649785814
>50	0.11690152987202873	4.847513470404952
>100	0.06125933460044069	6.293304009396373
>500	0.0018377793453704233	0.6787170963365112
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.034574412820422E-5	0.0
8	0.0	0.0	0.0	9.034574412820422E-5	0.0
9	0.0	0.0	0.0	9.034574412820422E-5	0.0
10	0.0	0.0	0.0	1.8069148825640844E-4	0.0
11	0.0	0.0	0.0	2.7103723238461265E-4	0.0
12	0.0	0.0	0.0	2.7103723238461265E-4	0.0
13	0.0	0.0	0.0	3.613829765128169E-4	0.0
14	0.0	0.0	0.0	4.517287206410211E-4	0.0
15	0.0	0.0	0.0	6.324202088974296E-4	0.0
16	0.0	0.0	0.0	0.0010841489295384506	0.0
17	0.0	0.0	0.0	0.0020779521149486974	0.0
18	0.0	0.0	0.0	0.002800718067974331	0.0
19	0.0	0.0	0.0	0.003162101044487148	0.0
20	0.0	0.0	0.0	0.0034331382768717604	0.0
21	0.0	0.0	0.0	0.004155904229897395	0.0
22	0.0	0.0	0.0	0.005149707415307641	0.0
23	0.0	0.0	0.0	0.006866276553743521	0.0
24	0.0	0.0	0.0	0.009124920156948626	0.0
25	0.0	0.0	0.0	0.010570452062999895	0.0
26	0.0	0.0	0.0	0.014274627572256268	0.0
27	0.0	0.0	0.0	0.030536861515333028	0.0
28	0.0	0.0	0.0	0.09034574412820423	0.0
29	0.0	0.0	0.0	0.17843284465320333	0.0
30	0.0	0.0	0.0	0.29841199285545855	0.0
31	0.0	0.0	0.0	0.6206752621607631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	445	0.0	22.86517	1
GTCTATA	45	0.0038257209	20.555555	1
TCTAGAC	210	0.0	20.261906	3
GTATGTA	65	6.901838E-5	19.923075	1
GTTCTAG	265	0.0	19.547169	1
GTATTAA	195	0.0	18.97436	1
CTAGACA	290	0.0	18.5	4
ATACCGT	60	9.2365197E-4	18.5	6
TGCGTTA	60	9.2365197E-4	18.5	37
TACCGTC	50	0.0070347204	18.5	7
GTCTAGA	265	0.0	18.150942	1
GTATCAA	760	0.0	17.769737	1
ATACCGC	230	0.0	17.695652	27
CGCAATA	105	4.796857E-7	17.61905	36
GTGCTAC	95	3.606141E-6	17.526316	1
TCTATAT	65	0.0015798844	17.076921	2
GACGTAT	110	7.806957E-7	16.818182	26
ACGTATG	110	7.806957E-7	16.818182	27
TATTAAG	100	5.877393E-6	16.650002	2
GCGTTAT	190	1.8189894E-12	16.552631	1
>>END_MODULE