FastQCFastQC Report
Fri 10 Feb 2017
ERR1632087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812313
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48760.17338041676015437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41950.14916547340214265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30240.10752714936068637No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA28850.10258459851375007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.020.3183751
ATTCGCG1600.019.6562530
CTTATAC20400.019.4975537
TCTTATA35450.016.07334137
CAATTCG1851.8189894E-1116.028
CGAATTA2100.015.85714415
CGCGCTT3050.015.77049312
GTACTAT2400.015.4166661
TGTACTA1152.2119819E-514.4782615
TACCGTC2059.276846E-1114.4390247
TAAGAGT1956.7484507E-1014.230774
GTATCAA34650.013.7748912
CGAACGA1551.8900864E-613.12903216
AATTCGC2408.185452E-1113.10416629
TCGTAGC3400.013.0588232
TAGGACA10350.013.0483094
CCGATCG2703.6379788E-1213.01851818
CTCTTAT54400.012.88878637
CTTAGGA10050.012.88557152
CTAGCAC4200.012.773813
Produced by FastQC (version 0.11.5)