##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2812313
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.647569100594424	34.0	31.0	34.0	31.0	34.0
2	32.81081408790558	34.0	31.0	34.0	31.0	34.0
3	32.81376966219621	34.0	31.0	34.0	31.0	34.0
4	36.30352631446073	37.0	37.0	37.0	35.0	37.0
5	36.2049746952064	37.0	35.0	37.0	35.0	37.0
6	36.23820854933288	37.0	37.0	37.0	35.0	37.0
7	36.227487836524595	37.0	36.0	37.0	35.0	37.0
8	36.20859413585899	37.0	36.0	37.0	35.0	37.0
9	37.97030700352344	39.0	38.0	39.0	35.0	39.0
10	37.899058532958456	39.0	38.0	39.0	35.0	39.0
11	38.00990074717857	39.0	38.0	39.0	35.0	39.0
12	37.974767744557596	39.0	38.0	39.0	35.0	39.0
13	37.9987149367798	39.0	38.0	39.0	35.0	39.0
14	39.3738943709324	40.0	39.0	41.0	37.0	41.0
15	39.39947829420125	40.0	39.0	41.0	37.0	41.0
16	39.27396808250006	40.0	39.0	41.0	36.0	41.0
17	39.32749413027639	40.0	39.0	41.0	36.0	41.0
18	39.34116188347456	40.0	39.0	41.0	36.0	41.0
19	39.39920912074865	40.0	39.0	41.0	37.0	41.0
20	39.378271906434314	40.0	39.0	41.0	37.0	41.0
21	39.34969649537587	40.0	39.0	41.0	36.0	41.0
22	39.3364330357254	40.0	39.0	41.0	36.0	41.0
23	39.29044064440907	40.0	39.0	41.0	36.0	41.0
24	39.23829886644908	40.0	39.0	41.0	36.0	41.0
25	39.17933423484513	40.0	39.0	41.0	36.0	41.0
26	39.08977592465703	40.0	39.0	41.0	36.0	41.0
27	39.02041060152266	40.0	39.0	41.0	36.0	41.0
28	38.953708210999274	40.0	39.0	41.0	35.0	41.0
29	38.823281761311776	40.0	38.0	41.0	35.0	41.0
30	38.77756743292799	40.0	38.0	41.0	35.0	41.0
31	38.703604826347565	40.0	38.0	41.0	35.0	41.0
32	38.6292901963615	40.0	38.0	41.0	35.0	41.0
33	38.491882660287104	40.0	38.0	41.0	35.0	41.0
34	38.49047492224372	40.0	38.0	41.0	34.0	41.0
35	38.46296695993654	40.0	38.0	41.0	34.0	41.0
36	38.437717636692646	40.0	38.0	41.0	34.0	41.0
37	38.4069600360984	40.0	38.0	41.0	34.0	41.0
38	38.32895200498664	40.0	38.0	41.0	34.0	41.0
39	38.138005975864	40.0	38.0	41.0	34.0	41.0
40	38.144832740879124	40.0	38.0	41.0	34.0	41.0
41	38.1450315096506	40.0	38.0	41.0	34.0	41.0
42	38.01511531611168	40.0	37.0	41.0	34.0	41.0
43	37.164590498994954	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	5.0
12	2.0
13	3.0
14	9.0
15	1.0
16	9.0
17	19.0
18	42.0
19	90.0
20	192.0
21	375.0
22	695.0
23	1237.0
24	2003.0
25	3090.0
26	4653.0
27	6735.0
28	9947.0
29	14333.0
30	19828.0
31	26801.0
32	36784.0
33	50663.0
34	73998.0
35	109108.0
36	175994.0
37	319396.0
38	735847.0
39	1220445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99909007283328	17.715773457648563	12.432151044353883	26.852985425164267
2	19.550846580732657	20.149641949526956	33.565502844100216	26.734008625640175
3	20.4976828681587	21.067747437785197	29.629738937308897	28.8048307567472
4	15.793832336585577	15.29513251192168	32.472985759408715	36.438049392084025
5	16.384058246717203	34.62139527143672	32.797487335157925	16.197059146688154
6	35.99183305698903	32.92019771625704	15.208620093140416	15.879349133613507
7	30.50225917243209	29.37066393392201	19.956882466496438	20.170194427149468
8	27.910655748488878	32.298574162975456	19.458111525993015	20.332658562542647
9	27.029103801746107	14.102022072223114	18.785177894494673	40.0836962315361
10	17.99145400956437	26.145880632774514	31.68367105652891	24.1789943011322
11	36.98418348171061	20.60165422554317	20.534805336390367	21.879356956355853
12	22.717777146427157	24.54847664538051	27.345675961388366	25.388070246803967
13	31.616431030258724	18.477815236070807	23.454003875102096	26.451749858568373
14	23.8904417822625	19.93536992504035	23.413005593616358	32.76118269908079
15	26.832397389621992	26.30770472561198	20.975119056804843	25.884778827961185
16	26.5009264615994	24.95902838695408	22.534369396294082	26.00567575515243
17	25.436215670161893	25.219490149211698	23.84752337310961	25.4967708075168
18	24.7908394264792	23.93631149875565	25.12846187462064	26.144387200144507
19	26.080880755449343	24.279267634861412	24.856088209242714	24.78376340044654
20	26.323954694943275	23.57699871955931	24.82202372211059	25.27702286338683
21	26.22250794986191	24.271800471711362	23.795146557299987	25.71054502112674
22	26.578869421717993	23.85292817691345	23.689006166810024	25.879196234558528
23	25.71911447978941	24.215476726808145	24.014432248473053	26.050976544929387
24	26.345822815596986	24.16729574553046	23.94815228603644	25.538729152836115
25	26.350374229326533	23.394906612457433	24.141267348264577	26.113451809951453
26	26.026121559015657	23.962233222262245	24.10126468853218	25.91038053018992
27	25.804880182255673	23.817868068027988	24.063182156466937	26.3140695932494
28	25.258106050073376	24.10279367908195	24.612445343032586	26.02665492781209
29	25.196341943446548	24.471209285737398	24.731635490075252	25.600813280740798
30	25.05919504692401	25.156232609954866	24.25306144799672	25.531510895124406
31	25.81337852507882	24.206871710225712	24.35315699212712	25.626592772568348
32	24.811285230342424	24.264297750641553	24.74393853031295	26.18047848870307
33	25.054928096552555	23.94384977774522	24.83350893019376	26.16771319550847
34	25.592243822078125	23.728831036943614	24.648323284072575	26.03060185690569
35	25.26685329833486	23.78305686458086	25.07736514392246	25.87272469316182
36	24.838309249361647	23.917536917121247	25.25170562451619	25.99244820900092
37	25.403217920622634	22.527293370261418	25.607889306773462	26.461599402342483
38	24.414814424994656	23.049070284850938	26.60119979532861	25.934915494825788
39	24.23755819498043	22.633860455788525	27.160774778625278	25.967806570605763
40	23.84538989792388	22.324222090499884	27.93942210557644	25.890965905999796
41	23.185861602175862	22.56985620021669	28.402528452558446	25.841753745049008
42	23.465382409425978	22.131427049549607	28.17357100721008	26.229619533814336
43	22.453332897156184	22.12868908972792	28.79907037374574	26.618907639370153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	90.0
2	104.0
3	290.5
4	477.0
5	477.0
6	625.5
7	774.0
8	811.0
9	848.0
10	1290.0
11	1732.0
12	1732.0
13	3122.0
14	4512.0
15	7597.5
16	10683.0
17	10337.5
18	9992.0
19	9992.0
20	12322.0
21	14652.0
22	15654.5
23	16657.0
24	19882.5
25	23108.0
26	23108.0
27	25878.5
28	28649.0
29	37426.5
30	46204.0
31	52369.5
32	58535.0
33	58535.0
34	67001.5
35	75468.0
36	84486.5
37	93505.0
38	108546.5
39	123588.0
40	123588.0
41	137062.5
42	150537.0
43	150772.5
44	151008.0
45	164012.5
46	177017.0
47	177017.0
48	189305.5
49	201594.0
50	213642.5
51	225691.0
52	239495.5
53	253300.0
54	253300.0
55	232257.5
56	211215.0
57	202415.0
58	193615.0
59	181910.0
60	170205.0
61	170205.0
62	160636.0
63	151067.0
64	136279.0
65	121491.0
66	106800.5
67	92110.0
68	92110.0
69	80343.5
70	68577.0
71	60129.0
72	51681.0
73	42436.0
74	33191.0
75	33191.0
76	26887.5
77	20584.0
78	17081.0
79	13578.0
80	10782.5
81	7987.0
82	7987.0
83	6049.0
84	4111.0
85	3229.5
86	2348.0
87	1739.5
88	1131.0
89	1131.0
90	841.5
91	552.0
92	333.0
93	114.0
94	73.5
95	33.0
96	33.0
97	21.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2812313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.46970137081897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60807046276292	42.51812717537979
2	9.780352178423133	10.067836118497546
3	2.8567263962443894	4.411045635385038
4	1.3370521857354618	2.7527070686801993
5	0.7401644205521832	1.9048020845563067
6	0.4937014515831549	1.5246399767594894
7	0.34136358637808795	1.2298917294826366
8	0.25614621523636033	1.0547015364384789
9	0.18228770432761207	0.8444064334782903
>10	1.1601259947373332	11.25050433701864
>50	0.12056024622735254	4.3257419802050485
>100	0.10907679201361554	11.456351155431408
>500	0.010831348123034726	3.6833402648278293
>1k	0.0035410176556002616	2.9759045038593097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4876	0.17338041676015437	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4195	0.14916547340214265	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3024	0.10752714936068637	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2885	0.10258459851375007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.555791976213174E-5
2	0.0	0.0	0.0	0.0	3.555791976213174E-5
3	0.0	0.0	0.0	0.0	3.555791976213174E-5
4	0.0	0.0	0.0	0.0	3.555791976213174E-5
5	0.0	3.555791976213174E-5	0.0	0.0	3.555791976213174E-5
6	0.0	3.555791976213174E-5	0.0	0.0	3.555791976213174E-5
7	0.0	3.555791976213174E-5	0.0	0.0	3.555791976213174E-5
8	0.0	3.555791976213174E-5	0.0	0.0	3.555791976213174E-5
9	0.0	7.111583952426347E-5	0.0	0.0	3.555791976213174E-5
10	0.0	7.111583952426347E-5	0.0	3.555791976213174E-5	3.555791976213174E-5
11	0.0	7.111583952426347E-5	0.0	3.555791976213174E-5	3.555791976213174E-5
12	0.0	7.111583952426347E-5	0.0	3.555791976213174E-5	3.555791976213174E-5
13	0.0	7.111583952426347E-5	0.0	3.555791976213174E-5	3.555791976213174E-5
14	0.0	7.111583952426347E-5	0.0	1.0667375928639522E-4	3.555791976213174E-5
15	0.0	7.111583952426347E-5	0.0	1.4223167904852695E-4	3.555791976213174E-5
16	0.0	1.4223167904852695E-4	0.0	3.555791976213174E-4	7.111583952426347E-5
17	0.0	1.4223167904852695E-4	0.0	7.467163150047665E-4	7.111583952426347E-5
18	0.0	1.777895988106587E-4	0.0	9.60063833577557E-4	7.111583952426347E-5
19	0.0	1.777895988106587E-4	0.0	0.0011378534323882156	7.111583952426347E-5
20	0.0	1.777895988106587E-4	0.0	0.0013156430311988745	7.111583952426347E-5
21	0.0	2.1334751857279044E-4	0.0	0.0017067801485823235	7.111583952426347E-5
22	0.0	2.1334751857279044E-4	0.0	0.0023823806240628265	7.111583952426347E-5
23	0.0	2.844633580970539E-4	0.0	0.0035202340564510423	7.111583952426347E-5
24	0.0	2.844633580970539E-4	0.0	0.005404803803844025	7.111583952426347E-5
25	0.0	2.844633580970539E-4	0.0	0.006471541396707976	7.111583952426347E-5
26	0.0	2.844633580970539E-4	0.0	0.009956217533396887	7.111583952426347E-5
27	0.0	2.844633580970539E-4	0.0	0.02492610175325435	7.111583952426347E-5
28	0.0	3.2002127785918567E-4	0.0	0.08274327928648056	7.111583952426347E-5
29	0.0	3.555791976213174E-4	0.0	0.163566430905806	7.111583952426347E-5
30	0.0	3.555791976213174E-4	0.0	0.2719825282605457	7.111583952426347E-5
31	0.0	3.555791976213174E-4	0.0	0.6056936052281521	7.111583952426347E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2340	0.0	20.318375	1
ATTCGCG	160	0.0	19.65625	30
CTTATAC	2040	0.0	19.49755	37
TCTTATA	3545	0.0	16.073341	37
CAATTCG	185	1.8189894E-11	16.0	28
CGAATTA	210	0.0	15.857144	15
CGCGCTT	305	0.0	15.770493	12
GTACTAT	240	0.0	15.416666	1
TGTACTA	115	2.2119819E-5	14.478261	5
TACCGTC	205	9.276846E-11	14.439024	7
TAAGAGT	195	6.7484507E-10	14.23077	4
GTATCAA	3465	0.0	13.774891	2
CGAACGA	155	1.8900864E-6	13.129032	16
AATTCGC	240	8.185452E-11	13.104166	29
TCGTAGC	340	0.0	13.058823	2
TAGGACA	1035	0.0	13.048309	4
CCGATCG	270	3.6379788E-12	13.018518	18
CTCTTAT	5440	0.0	12.888786	37
CTTAGGA	1005	0.0	12.8855715	2
CTAGCAC	420	0.0	12.77381	3
>>END_MODULE