FastQCFastQC Report
Fri 10 Feb 2017
ERR1632086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10314
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT350.3393445801822765RNA PCR Primer, Index 26 (100% over 22bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC170.16482451037424858RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC160.15512895094046927No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG150.14543339150668994No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT140.1357378320729106No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC120.11634671320535195No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA120.11634671320535195No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT110.10665115377157261No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)