##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10314
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5043630017452	34.0	31.0	34.0	31.0	34.0
2	32.64649990304441	34.0	31.0	34.0	31.0	34.0
3	32.66560015512895	34.0	31.0	34.0	31.0	34.0
4	36.19866201279814	37.0	37.0	37.0	35.0	37.0
5	36.06069420205546	37.0	35.0	37.0	35.0	37.0
6	36.106554198177236	37.0	35.0	37.0	35.0	37.0
7	36.095986038394415	37.0	35.0	37.0	35.0	37.0
8	36.09365910413031	37.0	35.0	37.0	35.0	37.0
9	37.863486523172384	39.0	38.0	39.0	35.0	39.0
10	37.75644754702346	39.0	37.0	39.0	35.0	39.0
11	37.8732790382005	39.0	38.0	39.0	35.0	39.0
12	37.79193329455109	39.0	38.0	39.0	35.0	39.0
13	37.850979251502814	39.0	38.0	39.0	35.0	39.0
14	39.182955206515416	40.0	39.0	41.0	36.0	41.0
15	39.1818886949777	40.0	39.0	41.0	36.0	41.0
16	39.04789606360287	40.0	38.0	41.0	36.0	41.0
17	39.11586193523366	40.0	39.0	41.0	36.0	41.0
18	39.129338762846615	40.0	39.0	41.0	36.0	41.0
19	39.1834399844871	40.0	39.0	41.0	36.0	41.0
20	39.131665697110726	40.0	39.0	41.0	36.0	41.0
21	39.119643203412835	40.0	39.0	41.0	36.0	41.0
22	39.10190032964902	40.0	39.0	41.0	36.0	41.0
23	39.019875896839245	40.0	38.0	41.0	36.0	41.0
24	38.94299011052938	40.0	38.0	41.0	35.0	41.0
25	38.8796781074268	40.0	38.0	41.0	35.0	41.0
26	38.750630211363195	40.0	38.0	41.0	35.0	41.0
27	38.68440954043048	40.0	38.0	41.0	35.0	41.0
28	38.57329842931937	40.0	38.0	41.0	35.0	41.0
29	38.41128563118092	40.0	38.0	41.0	34.0	41.0
30	38.42146596858639	40.0	38.0	41.0	34.0	41.0
31	38.34719798332364	40.0	38.0	41.0	34.0	41.0
32	38.22280395578825	40.0	38.0	41.0	34.0	41.0
33	38.051677331782045	40.0	38.0	41.0	33.0	41.0
34	38.0695171611402	40.0	38.0	41.0	33.0	41.0
35	38.05613728912158	40.0	38.0	41.0	33.0	41.0
36	38.03674617025403	40.0	37.0	41.0	33.0	41.0
37	38.00911382586775	40.0	38.0	41.0	33.0	41.0
38	37.91109171999224	40.0	37.0	41.0	33.0	41.0
39	37.75101803374055	40.0	37.0	41.0	33.0	41.0
40	37.68712429707194	40.0	37.0	41.0	33.0	41.0
41	37.660558464223385	40.0	37.0	41.0	33.0	41.0
42	37.57727360868722	40.0	37.0	41.0	33.0	41.0
43	36.72047702152414	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	6.0
23	6.0
24	11.0
25	10.0
26	31.0
27	33.0
28	41.0
29	71.0
30	92.0
31	149.0
32	184.0
33	222.0
34	316.0
35	484.0
36	758.0
37	1299.0
38	2647.0
39	3949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.426410703897616	16.60849331006399	12.187318208260617	27.77777777777778
2	20.835757223191777	19.07116540624394	31.89839053713399	28.19468683343029
3	21.57261974015901	20.157068062827225	28.456466938142334	29.813845258871435
4	16.947837890246266	14.252472367655614	30.434361062633315	38.3653286794648
5	17.79135156098507	33.05216210975374	32.412255187124295	16.7442311421369
6	38.19080860965678	31.491177040915257	13.990692262943572	16.32732208648439
7	31.694783789024626	28.039557882489817	18.828776420399457	21.436881908086097
8	29.930191972076788	30.114407601318593	17.975567190226876	21.97983323637774
9	27.176653092883456	13.554392088423503	16.773317820438237	42.495636998254795
10	18.857863098700793	24.898196625945317	29.978669769245688	26.2652705061082
11	39.17006011246849	19.546247818499126	18.848167539267017	22.43552452976537
12	24.597634283498156	23.191778165600155	24.73337211557107	27.47721543533062
13	34.17684700407214	16.656971107232888	21.57261974015901	27.593562148535973
14	25.30541012216405	17.723482644948614	21.25266627884429	35.71844095404305
15	28.689160364553036	24.888501066511537	18.906340895869693	27.515997673065733
16	28.504944735311227	23.191778165600155	20.07950358735699	28.223773511731626
17	27.205739771184795	23.269342641070388	22.086484390149312	27.4384331975955
18	26.934264107038974	21.39809967035098	22.891215823152997	28.77642039945705
19	28.078340120224937	22.11557106845065	22.464611208066707	27.341477603257708
20	28.417684700407214	21.3884041109172	21.941050998642623	28.252860190032962
21	28.883071553228625	21.766530928834595	21.339926313748304	28.01047120418848
22	29.02850494473531	21.37870855148342	21.68896645336436	27.90382005041691
23	28.698855923986816	21.941050998642623	20.864843901493117	28.495249175877447
24	28.727942602288152	23.11421369012992	20.758192747721544	27.399650959860384
25	28.960636028698854	21.12662400620516	21.52414194299011	28.388598022105878
26	27.58386658910219	21.81500872600349	22.21252666278844	28.388598022105878
27	29.106069420205543	21.242970719410508	21.611401977894122	28.039557882489817
28	27.80686445607912	21.175101803374055	21.592010859026566	29.426022881520264
29	26.953655225906537	22.929998060888114	21.999224355245296	28.117122357960056
30	27.128175295714563	23.41477603257708	22.202831103354665	27.254217568353695
31	27.642039945704866	21.863486523172387	21.184797362807835	29.309676168314912
32	26.75974403723095	21.999224355245296	21.941050998642623	29.299980608881132
33	26.85669963156874	21.310839635446964	23.15299592786504	28.679464805119252
34	26.779135156098505	22.357960054295134	22.83304246655032	28.029862323056044
35	26.662788442893152	20.719410509986428	23.83168508822959	28.78611595889083
36	26.65309288345938	21.960442117510183	23.37599379484196	28.01047120418848
37	26.866395191002525	20.777583866589104	23.71533837502424	28.64068256738414
38	25.538103548574753	20.612759356214855	25.916230366492147	27.932906728718248
39	25.44114795423696	20.641846034516192	25.60597246461121	28.311033546635638
40	24.335854178786114	19.96315687415164	27.923211169284468	27.77777777777778
41	24.675198758968392	20.069808027923212	26.478572813651347	28.77642039945705
42	23.83168508822959	19.81772348264495	28.281946868334302	28.068644560791157
43	23.618382780686446	18.819080860965677	28.495249175877447	29.06728718247043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	1.5
11	2.0
12	2.0
13	4.0
14	6.0
15	8.0
16	10.0
17	6.0
18	2.0
19	2.0
20	5.0
21	8.0
22	8.5
23	9.0
24	13.5
25	18.0
26	18.0
27	24.0
28	30.0
29	48.5
30	67.0
31	75.5
32	84.0
33	84.0
34	112.5
35	141.0
36	161.0
37	181.0
38	230.5
39	280.0
40	280.0
41	349.5
42	419.0
43	435.5
44	452.0
45	517.5
46	583.0
47	583.0
48	669.0
49	755.0
50	829.5
51	904.0
52	971.0
53	1038.0
54	1038.0
55	912.0
56	786.0
57	826.0
58	866.0
59	841.5
60	817.0
61	817.0
62	780.5
63	744.0
64	694.0
65	644.0
66	543.5
67	443.0
68	443.0
69	380.0
70	317.0
71	278.0
72	239.0
73	212.5
74	186.0
75	186.0
76	145.5
77	105.0
78	87.0
79	69.0
80	61.0
81	53.0
82	53.0
83	39.5
84	26.0
85	20.0
86	14.0
87	11.0
88	8.0
89	8.0
90	6.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	10314.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.23657165018422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.55248737509402	85.3209230172581
2	3.3415708606425274	6.030637967810742
3	1.04222628129365	2.821407795229785
4	0.4405286343612335	1.59007174713981
5	0.23638121843773505	1.0665115377157262
6	0.161169012571183	0.8726003490401396
7	0.0644676050284732	0.4072134962187318
8	0.0322338025142366	0.2326934264107039
9	0.010744600838078867	0.08726003490401396
>10	0.11819060921886752	1.5706806282722512
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	35	0.3393445801822765	RNA PCR Primer, Index 26 (100% over 22bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	17	0.16482451037424858	RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	16	0.15512895094046927	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	15	0.14543339150668994	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	14	0.1357378320729106	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	12	0.11634671320535195	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	12	0.11634671320535195	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	11	0.10665115377157261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.01939111886755866	0.0
28	0.0	0.0	0.0	0.07756447547023464	0.0
29	0.0	0.0	0.0	0.18421562924180726	0.0
30	0.0	0.0	0.0	0.34904013961605584	0.0
31	0.0	0.0	0.0	0.736862516967229	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE