FastQCFastQC Report
Fri 10 Feb 2017
ERR1632085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354422
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT6170.1740862587536891TruSeq Adapter, Index 12 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.15941448329956945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5190.14643560501323283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3820.10778111968218677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2200.029.4318181
GCCGTCT1005.311449E-1022.236
CCGTCTT1107.2759576E-1121.86363637
CTACACT555.1334524E-420.1818184
ATGCCGT1101.7425918E-920.18181834
GTATCAA3250.019.9230752
CCTGATA500.00702551918.52
TATGCCG1258.520146E-917.76000233
CTTATAC2100.017.61904737
TAATACA650.001577020217.0769234
GTAGAAC1107.770195E-716.8181823
TGCCGTC1352.2040695E-816.44444335
CTCTTAT4400.015.97727337
TATAATA700.002587752915.8571422
TATACAC957.037675E-515.5789483
CGTATGC1601.0875738E-815.0312531
GCAAGCC1252.9446073E-614.80000128
CTATAAA750.00409696214.81
CTGGCGG750.00409696214.814
AATGTTG750.00409696214.85
Produced by FastQC (version 0.11.5)