##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632085.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354422
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77738966542709	31.0	31.0	34.0	30.0	34.0
2	31.962369717455463	33.0	31.0	34.0	30.0	34.0
3	31.994870521581618	33.0	31.0	34.0	30.0	34.0
4	35.77310945708788	37.0	35.0	37.0	35.0	37.0
5	35.23737521937126	37.0	35.0	37.0	32.0	37.0
6	35.21580206646314	37.0	35.0	37.0	32.0	37.0
7	35.21591210477905	37.0	35.0	37.0	32.0	37.0
8	35.16531705142457	37.0	35.0	37.0	32.0	37.0
9	36.82782953654119	38.0	37.0	39.0	33.0	39.0
10	36.59354385450113	38.0	35.0	39.0	32.0	39.0
11	36.772226329065354	38.0	37.0	39.0	33.0	39.0
12	36.705317390004005	38.0	35.0	39.0	32.0	39.0
13	36.77046853750614	38.0	37.0	39.0	33.0	39.0
14	37.87901146091382	39.0	37.0	40.0	33.0	41.0
15	37.8406588755777	39.0	37.0	40.0	33.0	41.0
16	37.72475749248071	39.0	37.0	40.0	33.0	41.0
17	37.76395934789601	39.0	37.0	40.0	33.0	41.0
18	37.77723448318671	39.0	37.0	40.0	33.0	41.0
19	37.858400437896066	39.0	37.0	40.0	33.0	41.0
20	37.82340825343799	39.0	37.0	40.0	33.0	41.0
21	37.791830078268276	39.0	37.0	40.0	33.0	41.0
22	37.75857875639774	39.0	37.0	40.0	33.0	41.0
23	37.721098013103024	39.0	37.0	40.0	33.0	41.0
24	37.643453284502655	39.0	37.0	40.0	33.0	41.0
25	37.54500002821495	39.0	37.0	40.0	32.0	41.0
26	37.44421621682627	39.0	37.0	40.0	32.0	41.0
27	37.34411802879054	39.0	36.0	40.0	32.0	41.0
28	37.275877343957205	39.0	36.0	40.0	32.0	41.0
29	37.117918752221925	39.0	36.0	40.0	31.0	41.0
30	37.0560095027961	39.0	36.0	40.0	31.0	41.0
31	36.96590222954557	39.0	36.0	40.0	31.0	41.0
32	36.86852678445469	39.0	36.0	40.0	31.0	41.0
33	36.72043496171231	38.0	36.0	40.0	30.0	41.0
34	36.72532179153664	38.0	36.0	40.0	30.0	41.0
35	36.69691215556596	38.0	36.0	40.0	30.0	41.0
36	36.663573367341755	38.0	35.0	40.0	30.0	41.0
37	36.65043930681504	38.0	35.0	40.0	30.0	41.0
38	36.58510758361501	38.0	35.0	40.0	30.0	41.0
39	36.41983849761019	38.0	35.0	40.0	30.0	41.0
40	36.422716422795425	38.0	35.0	40.0	30.0	41.0
41	36.41143890616271	38.0	35.0	40.0	30.0	41.0
42	36.29301792778101	38.0	35.0	40.0	30.0	41.0
43	35.43015106285727	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	3.0
17	11.0
18	19.0
19	49.0
20	60.0
21	156.0
22	223.0
23	386.0
24	641.0
25	968.0
26	1416.0
27	2045.0
28	2961.0
29	4211.0
30	5757.0
31	8066.0
32	11103.0
33	15068.0
34	21539.0
35	31806.0
36	47800.0
37	71564.0
38	89437.0
39	39131.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.13401538279226	19.962361252969625	13.310686131222102	25.59293723301601
2	19.134252388395755	21.548323749654365	34.44707157004927	24.870352291900616
3	20.33987732138524	22.658582142192078	30.681221820315898	26.32031871610679
4	15.662966745856636	16.38583383649999	34.40333839321487	33.54786102442851
5	15.352320115568446	35.54209388807693	34.08168793133609	15.023898065018537
6	34.355372973461016	34.91854343127684	15.532331514409375	15.193752080852768
7	28.442365315922824	30.476381263014147	21.433771041301046	19.64748237976198
8	26.758214783506666	33.179091591379766	20.681842549277416	19.38085107583615
9	26.11829965408468	14.277612563554182	20.41126115196009	39.19282663040104
10	16.65528663570546	26.992963190772585	34.128242603450126	22.223507570071835
11	35.296059499692454	21.653565523584877	21.35900141639063	21.691373560332032
12	21.425024405934167	25.978071338686647	29.187522219275326	23.409382036103853
13	31.01218321661748	19.938942842148624	24.050143614109736	24.998730327124164
14	23.220341852368083	21.32598992161886	24.84467668485591	30.60899154115715
15	25.419415273318247	27.76125635541812	22.33100654022606	24.488321831037577
16	25.011709205410497	26.521209180017042	23.67657763908561	24.79050397548685
17	23.09393886384028	26.635197589314437	25.576290410866143	24.694573135979144
18	23.19889848824283	25.36806405922883	26.921297210669763	24.511740241858575
19	24.25131622754795	25.71341508145657	26.715328055256165	23.319940635739318
20	25.05572453177286	25.276929761696508	26.2695318010733	23.397813905457337
21	25.36016387244584	25.415747329454717	25.086478830320917	24.137609967778523
22	24.838187245712735	25.66516751217475	25.704104147033764	23.792541095078747
23	24.754953134963404	25.541586018926594	25.32856312531389	24.374897720796113
24	25.056853129884715	25.440576487915536	25.48261676758215	24.019953614617602
25	24.425966785357566	25.54666471042994	25.68632872677204	24.34103977744045
26	24.47223930794364	25.46653424448821	25.316994994667375	24.74423145290078
27	23.994278007572895	25.585883494816912	25.85448984543849	24.565348652171707
28	24.089644548024673	25.478666674190652	25.96170666606475	24.469982111719926
29	23.918944083606547	25.710875735704896	26.154132644136084	24.21604753655247
30	24.05127221222159	26.1696508681741	25.82063190208283	23.958445017521484
31	24.5560377177489	25.881858349650983	25.503495832651474	24.05860809994865
32	23.572182313738992	25.93462031138022	26.066101991411365	24.427095383469425
33	23.601243715119264	25.407564993143765	26.650715813352445	24.340475478384523
34	24.07976931454594	24.900260141864784	26.435435723516033	24.584534820073245
35	23.736393339013944	25.072653503450688	26.8578135668779	24.333139590657467
36	23.8506638978393	25.29893742487769	26.225798624238898	24.62460005304411
37	23.885368289778853	24.525565568728805	26.565224506379405	25.02384163511294
38	23.392735213953987	24.738870611869466	27.410262342631103	24.458131831545447
39	23.154036713296577	24.26457725536225	28.04397018243788	24.537415848903287
40	22.46361681836906	23.46947988556015	29.355683337941773	24.71121995812901
41	22.571680087579214	23.680245582949137	28.862485963060987	24.885588366410662
42	22.243258037029303	23.407971288464037	29.33028988042503	25.018480794081633
43	21.617168234477543	23.09309241525639	29.78821856431034	25.501520785955723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	10.5
2	16.0
3	39.0
4	62.0
5	62.0
6	85.5
7	109.0
8	127.5
9	146.0
10	204.5
11	263.0
12	263.0
13	459.0
14	655.0
15	999.0
16	1343.0
17	1376.0
18	1409.0
19	1409.0
20	1800.0
21	2191.0
22	2514.5
23	2838.0
24	3471.5
25	4105.0
26	4105.0
27	5021.5
28	5938.0
29	7420.5
30	8903.0
31	10112.0
32	11321.0
33	11321.0
34	12814.5
35	14308.0
36	15795.0
37	17282.0
38	18530.5
39	19779.0
40	19779.0
41	20996.0
42	22213.0
43	22302.0
44	22391.0
45	22921.0
46	23451.0
47	23451.0
48	23811.5
49	24172.0
50	25039.5
51	25907.0
52	26517.5
53	27128.0
54	27128.0
55	26594.5
56	26061.0
57	24675.5
58	23290.0
59	21385.5
60	19481.0
61	19481.0
62	17576.5
63	15672.0
64	13469.0
65	11266.0
66	9573.0
67	7880.0
68	7880.0
69	6562.0
70	5244.0
71	4518.0
72	3792.0
73	3076.5
74	2361.0
75	2361.0
76	1914.0
77	1467.0
78	1208.5
79	950.0
80	721.5
81	493.0
82	493.0
83	388.5
84	284.0
85	211.0
86	138.0
87	104.0
88	70.0
89	70.0
90	48.0
91	26.0
92	16.0
93	6.0
94	6.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	354422.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.17076206425948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.25921820173782	78.4969946555733
2	4.600220641772731	7.744081541634952
3	0.8653382199652593	2.18508532253417
4	0.3610551630631337	1.215611528890377
5	0.1940624445491629	0.816719192287807
6	0.13026840362632117	0.6578874483673196
7	0.09537065684927065	0.5619194605900468
8	0.08044083652515416	0.5416613209126995
9	0.052088224610856396	0.3945875004063088
>10	0.3397760963715278	5.216313765388763
>50	0.01645294599519908	0.9315283020430587
>100	0.004700841710010752	0.7568667450450108
>500	0.0010073232235737327	0.4807432163262
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	617	0.1740862587536891	TruSeq Adapter, Index 12 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	565	0.15941448329956945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	519	0.14643560501323283	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.10778111968218677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	2.8214952796383973E-4	0.0	0.0	0.0	0.0
11	2.8214952796383973E-4	0.0	0.0	0.0	0.0
12	2.8214952796383973E-4	0.0	0.0	0.0	0.0
13	2.8214952796383973E-4	0.0	0.0	0.0	0.0
14	2.8214952796383973E-4	0.0	0.0	0.0	0.0
15	2.8214952796383973E-4	0.0	0.0	0.0	0.0
16	2.8214952796383973E-4	0.0	0.0	0.0	0.0
17	2.8214952796383973E-4	0.0	0.0	0.0	0.0
18	2.8214952796383973E-4	0.0	0.0	0.0	0.0
19	2.8214952796383973E-4	2.8214952796383973E-4	0.0	2.8214952796383973E-4	0.0
20	5.642990559276795E-4	2.8214952796383973E-4	0.0	2.8214952796383973E-4	0.0
21	5.642990559276795E-4	2.8214952796383973E-4	0.0	2.8214952796383973E-4	0.0
22	5.642990559276795E-4	2.8214952796383973E-4	0.0	0.0014107476398191987	0.0
23	5.642990559276795E-4	2.8214952796383973E-4	0.0	0.0016928971677830384	0.0
24	5.642990559276795E-4	2.8214952796383973E-4	0.0	0.002257196223710718	0.0
25	5.642990559276795E-4	2.8214952796383973E-4	0.0	0.003103644807602237	0.0
26	5.642990559276795E-4	2.8214952796383973E-4	0.0	0.003950093391493756	0.0
27	5.642990559276795E-4	2.8214952796383973E-4	0.0	0.017493270733758063	0.0
28	5.642990559276795E-4	5.642990559276795E-4	0.0	0.05784065323258714	0.0
29	5.642990559276795E-4	5.642990559276795E-4	0.0	0.11144906354571668	0.0
30	5.642990559276795E-4	5.642990559276795E-4	0.0	0.1881937351518811	0.0
31	5.642990559276795E-4	5.642990559276795E-4	0.0	0.40516672215607386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	220	0.0	29.431818	1
GCCGTCT	100	5.311449E-10	22.2	36
CCGTCTT	110	7.2759576E-11	21.863636	37
CTACACT	55	5.1334524E-4	20.181818	4
ATGCCGT	110	1.7425918E-9	20.181818	34
GTATCAA	325	0.0	19.923075	2
CCTGATA	50	0.007025519	18.5	2
TATGCCG	125	8.520146E-9	17.760002	33
CTTATAC	210	0.0	17.619047	37
TAATACA	65	0.0015770202	17.076923	4
GTAGAAC	110	7.770195E-7	16.818182	3
TGCCGTC	135	2.2040695E-8	16.444443	35
CTCTTAT	440	0.0	15.977273	37
TATAATA	70	0.0025877529	15.857142	2
TATACAC	95	7.037675E-5	15.578948	3
CGTATGC	160	1.0875738E-8	15.03125	31
GCAAGCC	125	2.9446073E-6	14.800001	28
CTATAAA	75	0.004096962	14.8	1
CTGGCGG	75	0.004096962	14.8	14
AATGTTG	75	0.004096962	14.8	5
>>END_MODULE