##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577306
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70852026481623	34.0	31.0	34.0	31.0	34.0
2	32.87439763314429	34.0	31.0	34.0	31.0	34.0
3	32.8699926901851	34.0	31.0	34.0	31.0	34.0
4	36.3405091926985	37.0	37.0	37.0	35.0	37.0
5	36.25295597135661	37.0	37.0	37.0	35.0	37.0
6	36.286205236044665	37.0	37.0	37.0	35.0	37.0
7	36.28081641278629	37.0	37.0	37.0	35.0	37.0
8	36.26012721156545	37.0	37.0	37.0	35.0	37.0
9	38.03432148635213	39.0	38.0	39.0	35.0	39.0
10	37.97057886112391	39.0	38.0	39.0	35.0	39.0
11	38.07474372343263	39.0	38.0	39.0	37.0	39.0
12	38.0496842229251	39.0	38.0	39.0	35.0	39.0
13	38.06265308172789	39.0	38.0	39.0	35.0	39.0
14	39.46827852126948	40.0	39.0	41.0	37.0	41.0
15	39.49133908187339	40.0	39.0	41.0	37.0	41.0
16	39.36567089203992	40.0	39.0	41.0	37.0	41.0
17	39.425689322473694	40.0	39.0	41.0	37.0	41.0
18	39.43936837656286	40.0	39.0	41.0	37.0	41.0
19	39.502440646728076	41.0	39.0	41.0	37.0	41.0
20	39.487441668716414	41.0	39.0	41.0	37.0	41.0
21	39.461342858033696	40.0	39.0	41.0	37.0	41.0
22	39.44378024825656	40.0	39.0	41.0	37.0	41.0
23	39.40347233529532	40.0	39.0	41.0	37.0	41.0
24	39.36023876419092	40.0	39.0	41.0	36.0	41.0
25	39.30834081059265	40.0	39.0	41.0	36.0	41.0
26	39.22926662809671	40.0	39.0	41.0	36.0	41.0
27	39.16643859582267	40.0	39.0	41.0	36.0	41.0
28	39.110573941722414	40.0	39.0	41.0	36.0	41.0
29	38.98744340090004	40.0	39.0	41.0	36.0	41.0
30	38.95438294422715	40.0	39.0	41.0	35.0	41.0
31	38.892886961160976	40.0	38.0	41.0	35.0	41.0
32	38.82705012592975	40.0	38.0	41.0	35.0	41.0
33	38.69581816229175	40.0	38.0	41.0	35.0	41.0
34	38.70942619685228	40.0	38.0	41.0	35.0	41.0
35	38.687247317713656	40.0	38.0	41.0	35.0	41.0
36	38.66327736070645	40.0	38.0	41.0	35.0	41.0
37	38.638633584269	40.0	38.0	41.0	35.0	41.0
38	38.58132775339248	40.0	38.0	41.0	35.0	41.0
39	38.41552140459306	40.0	38.0	41.0	34.0	41.0
40	38.42907920582845	40.0	38.0	41.0	34.0	41.0
41	38.4347330531815	40.0	38.0	41.0	34.0	41.0
42	38.307284178581206	40.0	38.0	41.0	34.0	41.0
43	37.50749862291402	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	1.0
14	0.0
15	1.0
16	0.0
17	0.0
18	4.0
19	18.0
20	29.0
21	69.0
22	103.0
23	189.0
24	374.0
25	540.0
26	865.0
27	1177.0
28	1713.0
29	2471.0
30	3474.0
31	4979.0
32	6727.0
33	9338.0
34	13843.0
35	20464.0
36	32516.0
37	59373.0
38	145566.0
39	273469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16827817483276	18.589101793502927	12.752335849618746	26.49028418204557
2	19.698218968796443	20.372558054134203	33.9693334210973	25.959889555972048
3	20.659407662487485	21.606046013725823	29.598687697685456	28.135858626101236
4	15.855542814382668	15.620312278063972	33.054047593477286	35.47009731407607
5	16.245457348442592	34.788136620786894	33.14117642983097	15.825229600939537
6	35.8794123047396	33.147239072519604	15.29674037685387	15.67660824588693
7	29.68685584421433	29.203576612749565	20.76888166760782	20.34068587542828
8	26.94896640603077	32.71471282127676	19.987493634225174	20.348827138467296
9	26.490630618770634	14.254658707860301	19.69648678517112	39.55822388819794
10	17.4460338191531	26.32243558875189	32.77464637471289	23.456884217382115
11	36.42886095069166	21.06751012461329	20.72800213405023	21.77562679064482
12	22.25474878140882	24.79898009028141	28.082507370441324	24.863763757868444
13	31.15644043193731	19.09576550390954	23.697311304576775	26.050482759576376
14	23.747717848073638	20.665643523538645	23.658163954644504	31.928474673743214
15	26.594215199564875	26.937360775741116	21.288536755204348	25.179887269489665
16	26.051002414663976	25.45980814334166	22.954377747676276	25.534811694318094
17	24.528586226368684	25.701967414161643	24.439725206389678	25.329721153079998
18	24.174527893352916	24.462416811881393	25.85942290570339	25.503632389062297
19	25.313438627001972	24.959553512348737	25.395024475754624	24.331983384894667
20	25.894413014934887	24.31154361811587	25.20742898913228	24.586614377816964
21	26.054813218639683	24.78927986197961	23.941895632472207	25.214011286908505
22	25.92334048147776	24.485628072460706	24.219911104336347	25.371120341725184
23	25.223884733572838	24.735582169594633	24.366973494126164	25.673559602706362
24	26.02831080917226	24.743030559183516	24.264428223507117	24.964230408137105
25	25.703353161061898	24.26148351134407	24.411663831659467	25.623499495934567
26	25.680315118845122	24.51282335537826	24.28226971484793	25.524591810928694
27	25.46846906146827	24.187865707267896	24.459991754805944	25.88367347645789
28	24.96388397141204	24.386893605817367	25.04200545291405	25.607216969856538
29	24.702324243988457	24.84193824418939	25.06071303606753	25.395024475754624
30	24.79014595379227	25.447682857964406	24.7606988321618	25.00147235608152
31	25.46639044111788	24.651051608678934	24.689159648436014	25.19339830176717
32	24.459298881355814	24.677380799783823	24.92785455200535	25.935465766855014
33	24.712544127377857	24.308945342677887	25.106096247050957	25.872414282893303
34	25.069200735831604	24.022961826137266	25.14974727440907	25.758090163622064
35	25.019833502509936	24.073195151271594	25.38497781072776	25.52199353549071
36	24.70682792141429	24.16101686107541	25.430014584986125	25.702140632524173
37	24.96215178778672	23.17540437826733	25.776278091687942	26.086165742258004
38	24.09588675676331	23.30826286232951	27.04129179326042	25.55455858764676
39	24.064361014782452	22.780812948419037	27.450260347198885	25.704565689599622
40	23.452726976681344	22.388126920558594	28.42773156696795	25.73141453579211
41	22.95004728861297	22.68381066540102	28.8091584012638	25.55698364472221
42	23.038908308591978	22.548353905900857	28.582069127984116	25.83066865752305
43	22.143022937575566	22.454989208496016	28.96938538660606	26.432602467322358
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	18.0
2	17.0
3	45.0
4	73.0
5	73.0
6	101.5
7	130.0
8	117.5
9	105.0
10	171.0
11	237.0
12	237.0
13	420.5
14	604.0
15	958.5
16	1313.0
17	1374.0
18	1435.0
19	1435.0
20	1849.5
21	2264.0
22	2744.5
23	3225.0
24	4063.0
25	4901.0
26	4901.0
27	5776.5
28	6652.0
29	9039.0
30	11426.0
31	12883.0
32	14340.0
33	14340.0
34	16323.5
35	18307.0
36	20463.5
37	22620.0
38	25675.5
39	28731.0
40	28731.0
41	30999.0
42	33267.0
43	33662.5
44	34058.0
45	35874.0
46	37690.0
47	37690.0
48	39552.5
49	41415.0
50	43507.0
51	45599.0
52	47594.0
53	49589.0
54	49589.0
55	46337.0
56	43085.0
57	41352.5
58	39620.0
59	36761.5
60	33903.0
61	33903.0
62	31347.5
63	28792.0
64	25768.5
65	22745.0
66	19764.5
67	16784.0
68	16784.0
69	14261.0
70	11738.0
71	10180.0
72	8622.0
73	7090.0
74	5558.0
75	5558.0
76	4549.0
77	3540.0
78	2863.0
79	2186.0
80	1750.5
81	1315.0
82	1315.0
83	997.5
84	680.0
85	536.0
86	392.0
87	298.0
88	204.0
89	204.0
90	155.0
91	106.0
92	61.0
93	16.0
94	9.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	577306.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.81098865032858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.23957351643845	65.52003978249782
2	5.108026123131102	7.336248119074991
3	1.3258630905407938	2.8563461804014354
4	0.5996592901665696	1.7224850592086247
5	0.3510621172807237	1.2605058859803184
6	0.2147788507879295	0.9254088969757571
7	0.16456749781287316	0.8272428302357245
8	0.11996921142699603	0.6892086144138305
9	0.08549570209698804	0.5525577803644813
>10	0.6681917282772605	9.571866109671493
>50	0.08592048337547072	4.315189329325752
>100	0.036892388664845496	4.422901411849829
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	5.196550875965259E-4	0.0
17	0.0	0.0	0.0	0.0012125285377252272	0.0
18	0.0	0.0	0.0	0.0012125285377252272	0.0
19	0.0	0.0	0.0	0.0013857469002574025	0.0
20	0.0	0.0	0.0	0.0020786203503861036	0.0
21	0.0	1.732183625321753E-4	0.0	0.003291148888111331	0.0
22	0.0	1.732183625321753E-4	0.0	0.004157240700772207	0.0
23	0.0	1.732183625321753E-4	0.0	0.005716205963561785	0.0
24	0.0	3.464367250643506E-4	0.0	0.00848769976407659	0.0
25	0.0	3.464367250643506E-4	0.0	0.009527009939269642	0.0
26	0.0	3.464367250643506E-4	0.0	0.0140306873651062	0.0
27	0.0	3.464367250643506E-4	0.0	0.028754248180341103	0.0
28	0.0	3.464367250643506E-4	0.0	0.08868780161647376	0.0
29	0.0	5.196550875965259E-4	0.0	0.16784859329367788	0.0
30	0.0	5.196550875965259E-4	0.0	0.2710867373628544	0.0
31	0.0	5.196550875965259E-4	0.0	0.5494486459520601	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAA	40	0.0019300287	23.125002	1
GGTATCA	355	0.0	22.408451	1
ATAGACG	55	5.1388325E-4	20.181818	3
TATATAA	60	9.229227E-4	18.5	5
TAACACA	60	9.229227E-4	18.5	4
ACAATAC	90	2.148443E-6	18.5	3
GCGATAA	70	1.2178638E-4	18.5	22
CCCTTAT	50	0.007030746	18.499998	1
TTTAGCG	75	2.0650218E-4	17.266666	18
CTAAACA	65	0.0015786462	17.076923	4
GTACTAT	65	0.0015786462	17.076923	6
TAGGACA	175	7.2759576E-12	16.914286	4
CTTATAC	330	0.0	16.257576	37
TCTTATA	615	0.0	16.243902	37
CGATAAA	80	3.3789172E-4	16.1875	23
TTATTGT	115	1.2395012E-6	16.086956	4
TATATTG	70	0.0025903985	15.857142	5
ATAGTAC	70	0.0025903985	15.857142	3
ATTGCCG	70	0.0025903985	15.857142	11
CGCAAAT	70	0.0025903985	15.857142	23
>>END_MODULE