FastQCFastQC Report
Fri 10 Feb 2017
ERR1632083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42231
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10682.5289479292462884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9442.235324761431176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7221.7096445738912174No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT3470.8216712841277735TruSeq Adapter, Index 11 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.6890672728564324No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT1860.4404347517226682No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC1620.3836044611778078TruSeq Adapter, Index 11 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.27231180886078943No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC1030.24389666358835926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.23442494849754922No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG950.22495323340673912No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC870.20600980322511897RNA PCR Primer, Index 11 (95% over 24bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.13497194004404348No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.13260401127134097No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11839643863512585No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG460.10892472354431579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC200.0018219237.037
ATATGCC250.005437295429.69
GCCGTCT701.833323E-723.78571536
CCGTCTT753.5674384E-722.237
GGTATCA3750.020.7200011
TCTTATA450.003765977720.55555537
GTACCTG500.006925913518.535
TATGCCG902.0541356E-618.533
ATGCCGT902.0541356E-618.534
GGAAGCA609.0374425E-418.58
GCGAGGA500.006925913518.59
TGCCGTC953.4429522E-617.52631635
CTCTTAT852.6239417E-517.41176437
GGGAAGC803.2870393E-416.18757
ACTGCCC700.002537442115.85714323
GTATGCC1201.8303454E-615.41666632
CGTATGC1101.38709565E-515.13636431
GTATCAA5250.014.7999992
ACCTGTC908.043661E-414.38888837
TGGGAAG908.043661E-414.3888886