FastQCFastQC Report
Fri 10 Feb 2017
ERR1632082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814663
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3600.027.751
TATACTC705.098913E-621.1428575
TATGCCG806.954997E-720.812533
GACCGTA909.469841E-820.55555516
GTACTAG851.2443979E-619.58823620
TAATACT801.6157284E-518.54
TCGTATG902.1506512E-618.530
CTTATAC4350.017.8620737
ATGCCGT1054.793019E-717.6190534
CGTATGC953.60378E-617.52631631
CTCGTAT953.60378E-617.52631629
TATATAC650.001579399717.0769233
ATATACA1950.017.0769234
CAGTATT650.001579399717.07692314
TATGTAC650.001579399717.0769233
CTAGATC1107.800736E-716.81818223
ACTAGAT1005.8735604E-616.65000222
ACCGTAC1005.8735604E-616.65000217
ACTCTAA904.4434004E-516.44444510
TCTTATA7500.016.27999937
Produced by FastQC (version 0.11.5)