FastQCFastQC Report
Fri 10 Feb 2017
ERR1632081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574475
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA451.3220869E-424.6666661
GGTATCA2900.021.6896551
CTTATAC7250.020.92413737
GGGTTAA555.1387906E-420.1818181
TATAGTG656.894453E-519.9230755
CGTTCGA500.007030703618.518
AGGACTC1258.551069E-917.765
CTGTACG650.00157863417.0769214
GAGCGAT650.00157863417.07692125
GTATCAA3950.016.3924052
TCTTATA12200.016.2254137
GTGTAAG1101.4492765E-515.1363641
CTATCCC750.00410108414.7999999
TAGCGCG750.00410108414.7999994
CGCGCGT750.00410108414.7999997
CAAGACT1152.2059312E-514.4782614
ATCCGGA908.265135E-414.38888811
TCTACAC1051.6533567E-414.0952383
AAGACTT1203.2947122E-513.8750015
ATAGACA800.00629356613.8754