##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632081.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574475
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.736978980808566	34.0	31.0	34.0	31.0	34.0
2	32.90625875799643	34.0	31.0	34.0	31.0	34.0
3	32.92473997998172	34.0	33.0	34.0	31.0	34.0
4	36.38634927542539	37.0	37.0	37.0	35.0	37.0
5	36.28182253361765	37.0	37.0	37.0	35.0	37.0
6	36.30878802384786	37.0	37.0	37.0	35.0	37.0
7	36.299090473910965	37.0	37.0	37.0	35.0	37.0
8	36.27812698550851	37.0	37.0	37.0	35.0	37.0
9	38.058448148309324	39.0	38.0	39.0	35.0	39.0
10	37.98792288611341	39.0	38.0	39.0	35.0	39.0
11	38.09790504373559	39.0	38.0	39.0	37.0	39.0
12	38.0645876670003	39.0	38.0	39.0	35.0	39.0
13	38.090303320423	39.0	38.0	39.0	37.0	39.0
14	39.49587188302363	40.0	39.0	41.0	37.0	41.0
15	39.509254536750944	40.0	39.0	41.0	37.0	41.0
16	39.39521302058401	40.0	39.0	41.0	37.0	41.0
17	39.44002088863745	40.0	39.0	41.0	37.0	41.0
18	39.45296139953871	40.0	39.0	41.0	37.0	41.0
19	39.50368075199095	40.0	39.0	41.0	37.0	41.0
20	39.48012010966535	40.0	39.0	41.0	37.0	41.0
21	39.45475434091997	40.0	39.0	41.0	37.0	41.0
22	39.44114191218069	40.0	39.0	41.0	37.0	41.0
23	39.404814830932594	40.0	39.0	41.0	37.0	41.0
24	39.353226859306325	40.0	39.0	41.0	36.0	41.0
25	39.28801775534183	40.0	39.0	41.0	36.0	41.0
26	39.21062883502328	40.0	39.0	41.0	36.0	41.0
27	39.14792114539362	40.0	39.0	41.0	36.0	41.0
28	39.09220418643109	40.0	39.0	41.0	36.0	41.0
29	38.946453718612645	40.0	39.0	41.0	35.0	41.0
30	38.917780582270765	40.0	38.0	41.0	35.0	41.0
31	38.860758083467516	40.0	38.0	41.0	35.0	41.0
32	38.79994081552722	40.0	38.0	41.0	35.0	41.0
33	38.66415770921276	40.0	38.0	41.0	35.0	41.0
34	38.669616606466775	40.0	38.0	41.0	35.0	41.0
35	38.64850515688237	40.0	38.0	41.0	35.0	41.0
36	38.63318160059185	40.0	38.0	41.0	35.0	41.0
37	38.60716132120632	40.0	38.0	41.0	35.0	41.0
38	38.551909134427085	40.0	38.0	41.0	35.0	41.0
39	38.37670220636233	40.0	38.0	41.0	34.0	41.0
40	38.3860516123417	40.0	38.0	41.0	34.0	41.0
41	38.40194786544236	40.0	38.0	41.0	34.0	41.0
42	38.29523129814178	40.0	38.0	41.0	34.0	41.0
43	37.44227338004265	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	5.0
19	13.0
20	31.0
21	60.0
22	96.0
23	216.0
24	305.0
25	492.0
26	745.0
27	1091.0
28	1537.0
29	2198.0
30	3146.0
31	4404.0
32	6317.0
33	8961.0
34	13791.0
35	21256.0
36	34435.0
37	63042.0
38	149857.0
39	262474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0927368466861	16.95130336394099	12.084076765742635	27.87188302363027
2	19.76273989294573	19.092214630749815	32.72309499978241	28.42195047652204
3	20.708298881587535	20.228034292179817	28.349014317420252	30.714652508812396
4	16.034466251795116	14.69550459114844	31.904434483658996	37.36559467339745
5	17.40284607685278	33.27351059663171	32.468079550894295	16.855563775621217
6	37.76108620914748	32.08790634927542	14.613168545193437	15.537838896383654
7	32.05639932111929	27.88650506984638	19.595108577396754	20.461987031637584
8	29.201618869402495	31.466817529048264	18.75068540841638	20.58087819313286
9	27.782584098524737	13.447930719352453	17.725575525479787	41.043909656643024
10	18.44397058183559	24.91666304016711	31.17542103659863	25.46394534139867
11	39.27081248096088	19.475521127986422	19.513468819356806	21.740197571695894
12	24.041603202924406	22.955394055441925	27.125636450672353	25.877366290961312
13	33.26846250924758	17.56072936159102	21.82357804952348	27.34723007963793
14	24.71978763218591	18.205840114887508	22.615605552896124	34.458766700030466
15	27.546020279385523	24.805605117716176	19.98328909003873	27.665085512859566
16	27.6885852299926	23.435658644849646	21.66134296531616	27.214413159841595
17	26.356760520475213	23.80695417555159	23.02328212715958	26.81300317681361
18	26.820140127942903	22.74650768092606	23.4903172461813	26.943034944949734
19	27.375081596240047	22.947386744418818	23.353322598894643	26.324209060446496
20	27.627485965446713	22.718308020366422	22.905087253579353	26.74911876060751
21	27.997562992297315	22.917620436050306	22.305757430697593	26.779059140954786
22	28.480438661386483	22.706471125810523	22.158144392706383	26.65494582009661
23	27.778058227076897	22.609164889681885	22.271117106923715	27.341659776317506
24	27.373166804473648	22.64415335741329	22.402715522868707	27.579964315244354
25	27.45202141085339	22.09634884024544	22.69341572740328	27.75821402149789
26	26.912746420644932	22.55172113669002	22.976978980808564	27.558553461856476
27	26.994212106706122	22.794551547064714	22.689237999912965	27.521998346316202
28	27.132251185865353	22.569824622481395	22.942860872970975	27.355063318682276
29	26.74528917707472	23.024152487053396	23.14391400844249	27.086644327429394
30	26.702293398320204	23.15801383872231	22.73989294573306	27.399799817224423
31	27.026241350798557	22.766700030462598	22.76530745463249	27.44175116410636
32	26.214195569868142	22.975760476957223	23.27324948866356	27.53679446451108
33	26.19678837199182	22.580443013185953	23.831498324557206	27.391270290265023
34	26.627616519430784	21.99138343705122	23.934723007963793	27.4462770355542
35	26.078767570390355	22.09147482484007	24.74415770921276	27.08559989555681
36	25.935680403846987	21.700857304495408	24.611166717437662	27.752295574219936
37	25.50607076025937	20.899778058227078	25.44584185560729	28.14830932590626
38	24.817964228208364	20.903955785717393	26.482092345184732	27.795987640889507
39	24.601070542669394	20.49262369989991	27.22712041429131	27.67918534313939
40	24.077810174507157	20.25919317637843	28.327951607989903	27.335045041124506
41	23.422603246442403	20.12237260107054	28.97166978545629	27.483354367030767
42	22.77227033378302	20.2759040863397	29.496670873406156	27.455154706471124
43	22.091822968797597	19.735236520301143	29.917054702119326	28.255885808781933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.5
2	7.0
3	14.5
4	22.0
5	22.0
6	32.5
7	43.0
8	47.0
9	51.0
10	75.0
11	99.0
12	99.0
13	154.0
14	209.0
15	352.0
16	495.0
17	519.0
18	543.0
19	543.0
20	721.5
21	900.0
22	1045.5
23	1191.0
24	1570.0
25	1949.0
26	1949.0
27	2602.5
28	3256.0
29	4287.0
30	5318.0
31	6640.5
32	7963.0
33	7963.0
34	9818.0
35	11673.0
36	13784.5
37	15896.0
38	18375.0
39	20854.0
40	20854.0
41	23553.5
42	26253.0
43	28718.5
44	31184.0
45	33805.5
46	36427.0
47	36427.0
48	38580.0
49	40733.0
50	42772.0
51	44811.0
52	46069.5
53	47328.0
54	47328.0
55	47270.0
56	47212.0
57	46850.0
58	46488.0
59	45061.0
60	43634.0
61	43634.0
62	40501.5
63	37369.0
64	33695.5
65	30022.0
66	26658.5
67	23295.0
68	23295.0
69	20296.0
70	17297.0
71	14684.5
72	12072.0
73	9976.0
74	7880.0
75	7880.0
76	6407.5
77	4935.0
78	4060.5
79	3186.0
80	2597.0
81	2008.0
82	2008.0
83	1492.5
84	977.0
85	757.0
86	537.0
87	386.5
88	236.0
89	236.0
90	164.5
91	93.0
92	56.0
93	19.0
94	11.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	574475.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.77365346969127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50404673053227	74.91350601677823
2	6.219356718258798	10.295977556031
3	1.497742675306345	3.719208995777272
4	0.636578739105397	2.1076779182753254
5	0.31798670640258686	1.3160460721868092
6	0.19768236891969612	0.9817735141215956
7	0.13901334745214491	0.8054649854765943
8	0.09564408080619102	0.6333448000863044
9	0.07345197400167784	0.5471899418411688
>10	0.3121739612685681	4.184021518972277
>50	0.0046366522334253014	0.25120949380328433
>100	0.001686045712878692	0.24457918665015782
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.740719787632186E-4	0.0	0.0	0.0
6	0.0	1.740719787632186E-4	0.0	0.0	0.0
7	0.0	1.740719787632186E-4	0.0	0.0	0.0
8	0.0	1.740719787632186E-4	0.0	0.0	0.0
9	0.0	1.740719787632186E-4	0.0	1.740719787632186E-4	0.0
10	0.0	1.740719787632186E-4	0.0	3.481439575264372E-4	0.0
11	0.0	3.481439575264372E-4	0.0	3.481439575264372E-4	0.0
12	0.0	3.481439575264372E-4	0.0	3.481439575264372E-4	0.0
13	0.0	3.481439575264372E-4	0.0	3.481439575264372E-4	0.0
14	0.0	3.481439575264372E-4	0.0	3.481439575264372E-4	0.0
15	0.0	3.481439575264372E-4	0.0	3.481439575264372E-4	0.0
16	0.0	3.481439575264372E-4	0.0	5.222159362896558E-4	0.0
17	0.0	3.481439575264372E-4	0.0	6.962879150528744E-4	0.0
18	0.0	5.222159362896558E-4	0.0	8.70359893816093E-4	0.0
19	0.0	5.222159362896558E-4	0.0	0.0012185038513425302	0.0
20	0.0	5.222159362896558E-4	0.0	0.0012185038513425302	0.0
21	0.0	5.222159362896558E-4	0.0	0.001740719787632186	0.0
22	0.0	5.222159362896558E-4	0.0	0.001740719787632186	0.0
23	0.0	6.962879150528744E-4	0.0	0.002088863745158623	0.0
24	0.0	6.962879150528744E-4	0.0	0.0040036555115540275	0.0
25	0.0	6.962879150528744E-4	0.0	0.0057443752991862135	0.0
26	0.0	6.962879150528744E-4	0.0	0.009748030810740242	0.0
27	0.0	6.962879150528744E-4	0.0	0.034814395752643716	0.0
28	0.0	8.70359893816093E-4	0.0	0.14447974237347144	0.0
29	0.0	8.70359893816093E-4	0.0	0.29070020453457507	0.0
30	0.0	8.70359893816093E-4	0.0	0.5004569389442535	0.0
31	0.0	8.70359893816093E-4	0.0	1.1351233735149484	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	45	1.3220869E-4	24.666666	1
GGTATCA	290	0.0	21.689655	1
CTTATAC	725	0.0	20.924137	37
GGGTTAA	55	5.1387906E-4	20.181818	1
TATAGTG	65	6.894453E-5	19.923075	5
CGTTCGA	50	0.0070307036	18.5	18
AGGACTC	125	8.551069E-9	17.76	5
CTGTACG	65	0.001578634	17.076921	4
GAGCGAT	65	0.001578634	17.076921	25
GTATCAA	395	0.0	16.392405	2
TCTTATA	1220	0.0	16.22541	37
GTGTAAG	110	1.4492765E-5	15.136364	1
CTATCCC	75	0.004101084	14.799999	9
TAGCGCG	75	0.004101084	14.799999	4
CGCGCGT	75	0.004101084	14.799999	7
CAAGACT	115	2.2059312E-5	14.478261	4
ATCCGGA	90	8.265135E-4	14.388888	11
TCTACAC	105	1.6533567E-4	14.095238	3
AAGACTT	120	3.2947122E-5	13.875001	5
ATAGACA	80	0.006293566	13.875	4
>>END_MODULE