FastQCFastQC Report
Fri 10 Feb 2017
ERR1632080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT652.4885145482388973TruSeq Adapter, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC451.7228177641653903No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT351.339969372128637No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC301.1485451761102603No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG281.0719754977029097No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC230.880551301684533RNA PCR Primer, Index 1 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110.4211332312404288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.2679938744257274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.22970903522205208No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC50.19142419601837674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40.1531393568147014No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC40.1531393568147014No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG40.1531393568147014No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC30.11485451761102604No Hit
TTAATAAACAGTCGCAGCCACCGATTTTTTGCAACCCATTCAT30.11485451761102604No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG30.11485451761102604No Hit
CGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGC30.11485451761102604No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers