FastQCFastQC Report
Fri 10 Feb 2017
ERR1632079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366609
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.16420764356576079No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT5420.14784143324359195RNA PCR Primer, Index 29 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5100.13911278773843522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3740.10201604434151917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG250.00549152329.5999984
GGTATCA2500.026.641
GCTCTAG551.8980792E-523.5454541
TATGCCG801.6117814E-518.533
ATACTTG609.2204666E-418.56
AGGACCG500.007025970618.4999985
GTAATAT500.007025970618.4999983
GTAATAG500.007025970618.4999981
GTATCAA3700.018.02
AATACTT1054.7752656E-717.6190475
CTCTAGA752.062431E-417.2666662
CTACACC650.001577160717.0769234
TAGAAGT904.4326825E-516.4444454
CTCTTAT8200.016.24390237
TCTTATA4700.016.13829837
TACACCA957.038732E-515.5789485
CTCGTAT1201.9266663E-615.41666729
CGTATGC1101.4464325E-515.13636431
GCCGTCT1001.09014116E-414.79999936
GCTGATC908.25508E-414.3888888
Produced by FastQC (version 0.11.5)