FastQCFastQC Report
Fri 10 Feb 2017
ERR1632078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490992
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86820.24869721844106202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78800.22572380572628067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64000.18332897927007566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.028.6498051
GTATCAA39650.018.4766712
CTTATAC37200.018.00268737
TCTTATA62000.015.15806637
AACGCCG1854.9094524E-914.05
GACGGAC2405.456968E-1213.8749997
GTACCGT950.001245962913.6315796
CGCGAAT950.001245962913.63157928
TAGGGCG1102.4586087E-413.4545454
TAATACT5050.013.1881184
GCTTTAT8300.013.1506021
GTCTTAG4600.012.8695651
TCTATAC3600.012.8472223
CGTGCGA1205.126084E-412.33333312
ACGGACC2552.237357E-1012.3333338
ATAACGC2401.1295924E-912.3333333
TATCAAC60600.012.3028061
ATCAACG61100.012.2626842
AAGACGG3800.012.1710535
TCAACGC63450.011.9542943
Produced by FastQC (version 0.11.5)