##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3490992
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.074932282858285	33.0	31.0	34.0	30.0	34.0
2	32.25073961785074	33.0	31.0	34.0	30.0	34.0
3	32.278115217680245	34.0	31.0	34.0	30.0	34.0
4	35.95786727669385	37.0	35.0	37.0	35.0	37.0
5	35.64564513467805	37.0	35.0	37.0	33.0	37.0
6	35.65125614724984	37.0	35.0	37.0	33.0	37.0
7	35.64769498182751	37.0	35.0	37.0	33.0	37.0
8	35.61085846086156	37.0	35.0	37.0	33.0	37.0
9	37.28190038819911	39.0	37.0	39.0	34.0	39.0
10	37.12034172521736	39.0	37.0	39.0	33.0	39.0
11	37.265447185212686	39.0	37.0	39.0	34.0	39.0
12	37.20897469830925	39.0	37.0	39.0	34.0	39.0
13	37.266058472777935	39.0	37.0	39.0	34.0	39.0
14	38.45946309816809	40.0	38.0	41.0	34.0	41.0
15	38.45148685531218	40.0	38.0	41.0	34.0	41.0
16	38.33465673940244	40.0	38.0	41.0	34.0	41.0
17	38.38512491578325	40.0	38.0	41.0	34.0	41.0
18	38.405850256889735	40.0	38.0	41.0	34.0	41.0
19	38.46858027746841	40.0	38.0	41.0	34.0	41.0
20	38.45086525549185	40.0	38.0	41.0	34.0	41.0
21	38.418027311434685	40.0	38.0	41.0	34.0	41.0
22	38.391944180909036	40.0	38.0	41.0	34.0	41.0
23	38.34392573801372	40.0	38.0	41.0	34.0	41.0
24	38.28262367831264	40.0	38.0	41.0	34.0	41.0
25	38.208281772057916	40.0	38.0	41.0	34.0	41.0
26	38.10362355456558	40.0	38.0	41.0	34.0	41.0
27	38.014199115896	40.0	38.0	41.0	34.0	41.0
28	37.942561884988564	40.0	37.0	41.0	33.0	41.0
29	37.79339339649017	40.0	37.0	41.0	33.0	41.0
30	37.734994523046744	40.0	37.0	41.0	33.0	41.0
31	37.63416959993034	39.0	37.0	40.0	33.0	41.0
32	37.548227552512294	39.0	37.0	40.0	33.0	41.0
33	37.40068467644727	39.0	37.0	40.0	32.0	41.0
34	37.39302295737143	39.0	37.0	40.0	32.0	41.0
35	37.359492659965994	39.0	36.0	40.0	32.0	41.0
36	37.33849433055131	39.0	36.0	40.0	32.0	41.0
37	37.31212789946239	39.0	36.0	40.0	32.0	41.0
38	37.24419964296681	39.0	36.0	40.0	32.0	41.0
39	37.05582080967244	39.0	36.0	40.0	31.0	41.0
40	37.06430120722133	39.0	36.0	40.0	31.0	41.0
41	37.062687339300695	39.0	36.0	40.0	31.0	41.0
42	36.94622359489795	39.0	36.0	40.0	31.0	41.0
43	36.09738177572449	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	1.0
13	4.0
14	4.0
15	6.0
16	33.0
17	62.0
18	122.0
19	260.0
20	492.0
21	925.0
22	1680.0
23	2916.0
24	4661.0
25	7272.0
26	10776.0
27	15964.0
28	23026.0
29	32117.0
30	44361.0
31	59421.0
32	79507.0
33	108589.0
34	153644.0
35	226183.0
36	359462.0
37	629174.0
38	1057435.0
39	672890.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.67726537328072	19.552780413131853	13.094959828037418	25.674994385550008
2	18.744643356386952	21.223824059178593	34.62471412137295	25.406818463061505
3	20.050031624248923	22.574758120327974	30.362000256660572	27.013209998762527
4	15.044119264667463	16.222151182242754	35.05324561041675	33.68048394267303
5	15.181071741212811	35.62878402471274	34.07240692616884	15.117737307905605
6	33.826660158487904	35.36504810094094	15.928882105716655	14.879409634854508
7	28.839739535352706	30.62536379344324	21.394749687194928	19.140146984009128
8	26.91206396348087	33.15206680508005	20.56045960575103	19.375409625688057
9	26.601063537240993	14.508053871220557	20.381427399432596	38.509455192105854
10	16.965406967417856	26.960388336610343	33.56702049159666	22.507184204375147
11	34.97977079294367	21.865332260858807	21.78249047835114	21.37240646784639
12	21.90466205594284	25.7437713979293	29.201413237268948	23.150153308858915
13	30.727054086632112	20.01488402150449	24.299597363729276	24.958464528134122
14	22.865277262165023	21.094663064252224	25.46857741295311	30.571482260629644
15	25.398396788076283	27.557496551123577	22.560951156576696	24.48315550422344
16	24.54288064825127	26.530997492976212	24.284701884163585	24.641419974608937
17	23.249007731899702	26.431999844170367	25.957664755462055	24.361327668467876
18	23.326406935335285	25.419279104621268	27.03684224999656	24.217471710046887
19	24.18137881725309	25.55668990361479	27.035639153570102	23.22629212556202
20	24.98255510181633	25.28736817500584	26.338702580813706	23.391374142364118
21	25.058636628213414	25.391922983495807	25.621714400949646	23.927725987341134
22	25.11882009468942	25.49876940422665	25.754112298166255	23.62829820291768
23	24.76525297107527	25.361931508293345	25.537898683239607	24.33491683739178
24	24.387996305921067	25.423203490583766	25.974422169973465	24.214378033521704
25	24.452963512949903	25.216299550385678	25.88378890584682	24.4469480308176
26	23.951558754646243	25.431453294650918	26.03116821808815	24.585819732614684
27	24.02199718590017	25.396019240376372	26.073534399391345	24.50844917433211
28	23.9948702259988	25.57473635001169	26.200632943300928	24.229760480688583
29	23.810767827597427	25.840248273270177	26.450762419392536	23.898221479739856
30	24.11217212757864	25.959383464642716	25.88046606809755	24.0479783396811
31	24.062644658022705	25.908366447130216	25.962047463872732	24.06694143097435
32	23.35138550876083	25.62400601319052	26.408940495996553	24.61566798205209
33	23.46043760627352	25.411258461778196	26.712980150054772	24.415323781893512
34	24.04167640601869	24.503923240156382	26.96852356006545	24.48587679375948
35	23.61171265932434	24.70847827780757	27.17806285434054	24.501746208527546
36	23.465249991979356	24.83947256252664	27.056951147410246	24.638326298083754
37	23.461497476934923	23.81022356969022	27.580527254144382	25.147751699230476
38	23.05972056080335	23.988997969631555	28.221233391540284	24.730048078024815
39	22.74688684477077	23.564620027774343	28.98199136520508	24.706501762249815
40	22.14800263077085	23.27467378899751	29.808776416560107	24.768547163671528
41	21.737603523582983	23.101943516341485	30.14014354659077	25.020309413484764
42	21.511421395408526	23.193522070517492	30.21433449288913	25.080722041184856
43	20.986613547094922	22.839038302006994	30.514106019148713	25.66024213174937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	526.0
1	515.5
2	505.0
3	1038.0
4	1571.0
5	1571.0
6	2006.5
7	2442.0
8	2489.0
9	2536.0
10	3554.5
11	4573.0
12	4573.0
13	7654.5
14	10736.0
15	15358.5
16	19981.0
17	20247.5
18	20514.0
19	20514.0
20	24731.5
21	28949.0
22	30688.5
23	32428.0
24	39647.5
25	46867.0
26	46867.0
27	55096.0
28	63325.0
29	75466.0
30	87607.0
31	101103.5
32	114600.0
33	114600.0
34	128278.5
35	141957.0
36	156696.5
37	171436.0
38	180984.5
39	190533.0
40	190533.0
41	199784.0
42	209035.0
43	212980.0
44	216925.0
45	221246.5
46	225568.0
47	225568.0
48	226870.5
49	228173.0
50	232551.0
51	236929.0
52	241149.5
53	245370.0
54	245370.0
55	240283.0
56	235196.0
57	227544.5
58	219893.0
59	205401.5
60	190910.0
61	190910.0
62	174666.0
63	158422.0
64	140036.5
65	121651.0
66	104844.5
67	88038.0
68	88038.0
69	76055.5
70	64073.0
71	54340.0
72	44607.0
73	35797.5
74	26988.0
75	26988.0
76	21605.5
77	16223.0
78	13171.5
79	10120.0
80	7814.5
81	5509.0
82	5509.0
83	4367.0
84	3225.0
85	2482.0
86	1739.0
87	1283.0
88	827.0
89	827.0
90	596.5
91	366.0
92	220.5
93	75.0
94	54.5
95	34.0
96	34.0
97	21.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3490992.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.74645082329084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.18647068003192	59.25013968237812
2	9.043129878354053	12.433661669417981
3	2.2247138854169894	4.588235511591341
4	0.7953414440597778	2.1870760588712255
5	0.41700808241278187	1.433391281525256
6	0.24163710023394547	0.9967015816989321
7	0.17668894052636538	0.8502716292640597
8	0.1261691300222085	0.6938943913991317
9	0.09791002299844771	0.6057869923053055
>10	0.5874712262364757	7.881050172272537
>50	0.06290008695885464	3.0060896955053042
>100	0.03918274921329895	4.765776645139782
>500	0.0011681714869891985	0.5257569327551506
>1k	8.34408191870976E-5	0.12358772542515009
>5k	1.251612287806464E-4	0.6585800304507534
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8682	0.24869721844106202	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7880	0.22572380572628067	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6400	0.18332897927007566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.8645153010949323E-5	0.0	2.8645153010949323E-5	0.0
5	0.0	8.593545903284797E-5	0.0	8.593545903284797E-5	0.0
6	0.0	8.593545903284797E-5	0.0	8.593545903284797E-5	0.0
7	0.0	8.593545903284797E-5	0.0	8.593545903284797E-5	0.0
8	0.0	8.593545903284797E-5	0.0	8.593545903284797E-5	0.0
9	0.0	8.593545903284797E-5	0.0	8.593545903284797E-5	0.0
10	0.0	1.145806120437973E-4	0.0	8.593545903284797E-5	0.0
11	0.0	1.145806120437973E-4	0.0	8.593545903284797E-5	0.0
12	0.0	1.145806120437973E-4	0.0	1.432257650547466E-4	0.0
13	0.0	1.145806120437973E-4	0.0	1.432257650547466E-4	0.0
14	0.0	1.145806120437973E-4	0.0	1.432257650547466E-4	0.0
15	0.0	1.145806120437973E-4	0.0	1.7187091806569594E-4	0.0
16	0.0	1.432257650547466E-4	0.0	3.1509668312044254E-4	0.0
17	0.0	1.432257650547466E-4	0.0	4.869676011861385E-4	0.0
18	0.0	1.432257650547466E-4	0.0	5.156127541970878E-4	0.0
19	0.0	1.432257650547466E-4	0.0	7.16128825273733E-4	2.8645153010949323E-5
20	0.0	1.432257650547466E-4	0.0	8.02064284306581E-4	5.729030602189865E-5
21	0.0	1.432257650547466E-4	0.0	8.879997433394291E-4	5.729030602189865E-5
22	0.0	1.432257650547466E-4	0.0	0.0012603867324817702	5.729030602189865E-5
23	0.0	2.0051607107664525E-4	0.0	0.002234321934854047	5.729030602189865E-5
24	0.0	2.0051607107664525E-4	0.0	0.004067611727554804	5.729030602189865E-5
25	0.0	2.0051607107664525E-4	0.0	0.0064738045804745474	5.729030602189865E-5
26	0.0	2.0051607107664525E-4	0.0	0.010627351767062199	5.729030602189865E-5
27	0.0	2.0051607107664525E-4	0.0	0.03597831218175235	5.729030602189865E-5
28	0.0	2.0051607107664525E-4	0.0	0.11541132148111483	5.729030602189865E-5
29	0.0	2.0051607107664525E-4	0.0	0.23228354576578805	5.729030602189865E-5
30	0.0	2.0051607107664525E-4	0.0	0.39536040185712257	5.729030602189865E-5
31	0.0	2.0051607107664525E-4	0.0	0.8984838693414364	5.729030602189865E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2570	0.0	28.649805	1
GTATCAA	3965	0.0	18.476671	2
CTTATAC	3720	0.0	18.002687	37
TCTTATA	6200	0.0	15.158066	37
AACGCCG	185	4.9094524E-9	14.0	5
GACGGAC	240	5.456968E-12	13.874999	7
GTACCGT	95	0.0012459629	13.631579	6
CGCGAAT	95	0.0012459629	13.631579	28
TAGGGCG	110	2.4586087E-4	13.454545	4
TAATACT	505	0.0	13.188118	4
GCTTTAT	830	0.0	13.150602	1
GTCTTAG	460	0.0	12.869565	1
TCTATAC	360	0.0	12.847222	3
CGTGCGA	120	5.126084E-4	12.333333	12
ACGGACC	255	2.237357E-10	12.333333	8
ATAACGC	240	1.1295924E-9	12.333333	3
TATCAAC	6060	0.0	12.302806	1
ATCAACG	6110	0.0	12.262684	2
AAGACGG	380	0.0	12.171053	5
TCAACGC	6345	0.0	11.954294	3
>>END_MODULE