FastQCFastQC Report
Fri 10 Feb 2017
ERR1632077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62661
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT2140.34152024385183766TruSeq Adapter, Index 6 (95% over 21bp)
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1170.1867190118255374No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT970.15480123202630025No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC960.15320534303633837No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG910.1452258980865291No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC900.14363000909656726No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC830.13245878616683424No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC820.13086289717687238No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG730.11649989626721566No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT670.1069245623274445No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA630.10054100636759708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT450.003786177120.5555555
GAAGAGG500.006962705418.4999986
AGAAGAG500.006962705418.4999985
AAGGGAA500.006962705418.4999985
TCTTATA803.319171E-416.187537
CTCTTAT1203.203617E-513.87500137
GTCTCTT2601.8189894E-1213.51923137
TCTCTTA1604.6860173E-410.4062536
GCTGTCT1952.6977042E-49.48717937
TGTCTCT3851.9153958E-99.12986936
CTGTCTC4554.783942E-108.53846237
CCTGTCT2759.7514375E-47.399999635
Produced by FastQC (version 0.11.5)