##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632077.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62661
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.307719315044444	33.0	31.0	34.0	30.0	34.0
2	32.48869312650612	34.0	31.0	34.0	31.0	34.0
3	32.50463605751584	34.0	31.0	34.0	31.0	34.0
4	36.101163403073684	37.0	35.0	37.0	35.0	37.0
5	35.93448875696207	37.0	35.0	37.0	35.0	37.0
6	35.92668485980115	37.0	35.0	37.0	35.0	37.0
7	35.937201768245004	37.0	35.0	37.0	35.0	37.0
8	35.8996026236415	37.0	35.0	37.0	35.0	37.0
9	37.644021001899105	39.0	37.0	39.0	35.0	39.0
10	37.53808589074544	39.0	37.0	39.0	35.0	39.0
11	37.63442970906944	39.0	37.0	39.0	35.0	39.0
12	37.58308996026236	39.0	37.0	39.0	35.0	39.0
13	37.63719059702207	39.0	37.0	39.0	35.0	39.0
14	38.90407111281339	40.0	38.0	41.0	36.0	41.0
15	38.92419527297681	40.0	38.0	41.0	36.0	41.0
16	38.823271253251626	40.0	38.0	41.0	36.0	41.0
17	38.86736566604427	40.0	38.0	41.0	36.0	41.0
18	38.85547629306905	40.0	38.0	41.0	36.0	41.0
19	38.911045147699525	40.0	38.0	41.0	36.0	41.0
20	38.88112222913774	40.0	38.0	41.0	36.0	41.0
21	38.83319768276919	40.0	38.0	41.0	35.0	41.0
22	38.84021959432502	40.0	38.0	41.0	35.0	41.0
23	38.791050254544295	40.0	38.0	41.0	35.0	41.0
24	38.75121686535485	40.0	38.0	41.0	35.0	41.0
25	38.672236319241634	40.0	38.0	41.0	35.0	41.0
26	38.56373182681413	40.0	38.0	41.0	35.0	41.0
27	38.49170935669715	40.0	38.0	41.0	34.0	41.0
28	38.439954676752684	40.0	38.0	41.0	34.0	41.0
29	38.32040663251464	40.0	38.0	41.0	34.0	41.0
30	38.27851454652814	40.0	38.0	41.0	34.0	41.0
31	38.177574567913055	40.0	38.0	41.0	34.0	41.0
32	38.11854263417437	40.0	38.0	41.0	34.0	41.0
33	37.96445955219355	40.0	38.0	41.0	33.0	41.0
34	37.96908763026444	40.0	38.0	41.0	33.0	41.0
35	37.952394631429435	40.0	38.0	41.0	33.0	41.0
36	37.93171191011953	40.0	37.0	41.0	33.0	41.0
37	37.93557396147524	40.0	37.0	41.0	33.0	41.0
38	37.83749062415218	40.0	37.0	41.0	33.0	41.0
39	37.70043567769426	40.0	37.0	41.0	33.0	41.0
40	37.731108664081326	40.0	37.0	41.0	33.0	41.0
41	37.71824579882223	40.0	37.0	41.0	33.0	41.0
42	37.6358819680503	40.0	37.0	41.0	33.0	41.0
43	36.730645856274236	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	7.0
21	12.0
22	19.0
23	35.0
24	51.0
25	95.0
26	136.0
27	208.0
28	326.0
29	429.0
30	593.0
31	808.0
32	1123.0
33	1516.0
34	2177.0
35	3180.0
36	4985.0
37	9194.0
38	19308.0
39	18457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18892133863169	17.736710234436092	12.94265970859067	27.131708718341553
2	19.31344855651841	20.22470116978663	33.26471010676497	27.19714016692999
3	20.218317613826784	20.786454094253205	30.213370357957903	28.78185793396211
4	16.23178691690206	15.255102855045402	31.911396243277316	36.601713984775216
5	15.993999457397742	34.87974976460637	33.52005234515887	15.606198432837012
6	36.951213673576866	33.231196437975775	14.776336158056846	15.041253730390514
7	31.068766856577458	29.101035731954482	19.431544341775584	20.39865306969247
8	29.07869328609502	32.49708750259332	19.230462329040392	19.193756882271266
9	27.596112414420453	13.687939866902859	17.318587319066086	41.397360399610605
10	17.593080225339524	26.437497007708146	31.496465105887232	24.472957661065095
11	37.95343195927291	19.89913981583441	19.97733837634254	22.170089848550134
12	23.134006798487096	24.187293531861922	27.14128405228132	25.537415617369657
13	32.888080305133975	17.264327093407385	22.803657777564993	27.04393482389365
14	24.72670401046903	18.668709404573818	22.5355484272514	34.06903815770575
15	27.248208614608764	26.206093104163674	20.05394104786071	26.491757233366847
16	27.387050956735447	24.08834841448429	21.483857582866538	27.04074304591373
17	26.20768899315364	25.258135044126327	22.348829415425865	26.18534654729417
18	25.752860631014507	23.49627359920844	23.646287164264855	27.104578605512202
19	27.13968816329136	23.416479149710344	23.74363639265253	25.700196294345766
20	27.24022916965896	23.140390354446943	23.646287164264855	25.973093311629242
21	26.85242814509823	23.646287164264855	22.2929733007772	27.208311389859723
22	27.0375512679338	23.465951708399164	22.683966103317854	26.812530920349182
23	26.20768899315364	23.612773495475654	22.85472622524377	27.324811286126938
24	27.621646638259843	23.430842150620002	22.58661687493018	26.36089433618998
25	27.13011282935159	22.90260289494263	23.004739790300185	26.962544485405594
26	27.117345717431895	22.942500119691676	23.004739790300185	26.935414372576243
27	26.81891447630903	22.773335886755717	23.39254081486092	27.01520882207434
28	26.247586217902686	23.004739790300185	23.346260034152024	27.401413957645104
29	25.877339972231532	23.702143278913518	23.312746365362823	27.107770383492124
30	25.736901741114888	24.33092354095849	23.18028757919599	26.75188713873063
31	26.525270902156045	23.307958698392937	23.20582180303538	26.960948596415633
32	25.60284706595809	23.64149949729497	24.024512854885813	26.731140581861123
33	25.802333189703326	23.355835368091796	23.900033513668788	26.941797928536094
34	25.856593415362028	23.10368490767782	24.121862083273488	26.91785959368666
35	25.79116196677359	22.851534447263848	24.45061521520563	26.90668837075693
36	25.80073730071336	22.93132889676194	24.683615007740062	26.584318794784632
37	26.67688035620242	21.585994478224094	24.359649542777802	27.37747562279568
38	25.14323103684908	22.356808860375672	25.657107291616793	26.84285281115846
39	25.01875169563205	21.550884920444936	26.459839453567607	26.970523930355405
40	24.096327859434098	21.15350856194443	27.854646430794272	26.895517147827196
41	23.29040391950336	21.150316783964506	28.312666570913326	27.246612725618807
42	23.53936260193741	20.15607794321827	28.77866615598219	27.52589329886213
43	22.17487751552002	20.35396817797354	29.881425448045835	27.589728858460603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	2.0
7	1.0
8	4.0
9	7.0
10	8.0
11	9.0
12	9.0
13	11.5
14	14.0
15	25.0
16	36.0
17	35.5
18	35.0
19	35.0
20	55.0
21	75.0
22	93.0
23	111.0
24	167.0
25	223.0
26	223.0
27	268.5
28	314.0
29	456.5
30	599.0
31	744.5
32	890.0
33	890.0
34	1104.5
35	1319.0
36	1515.0
37	1711.0
38	2115.0
39	2519.0
40	2519.0
41	2898.5
42	3278.0
43	3363.5
44	3449.0
45	3803.0
46	4157.0
47	4157.0
48	4473.0
49	4789.0
50	5265.5
51	5742.0
52	6139.0
53	6536.0
54	6536.0
55	6049.0
56	5562.0
57	5184.5
58	4807.0
59	4453.5
60	4100.0
61	4100.0
62	3770.0
63	3440.0
64	3080.5
65	2721.0
66	2366.5
67	2012.0
68	2012.0
69	1772.0
70	1532.0
71	1287.0
72	1042.0
73	864.5
74	687.0
75	687.0
76	541.5
77	396.0
78	321.5
79	247.0
80	198.5
81	150.0
82	150.0
83	116.5
84	83.0
85	63.5
86	44.0
87	28.0
88	12.0
89	12.0
90	8.5
91	5.0
92	3.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	62661.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.46951054084678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.6219582333459	75.33713154912944
2	3.571782718203994	5.748392141842613
3	0.9658290859330068	2.331593814334275
4	0.4184598298395573	1.3469303075278085
5	0.281617515816195	1.1330811828729195
6	0.1804731967554489	0.8713553885191747
7	0.14874164567756776	0.8378417197299756
8	0.1070939848878488	0.6894240436635227
9	0.08131209963707038	0.5888830372959256
>10	0.5810840291136982	8.545985541245752
>50	0.03768121690498384	2.0411420181612168
>100	0.003966443884735141	0.5282392556773751
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	214	0.34152024385183766	TruSeq Adapter, Index 6 (95% over 21bp)
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	117	0.1867190118255374	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	97	0.15480123202630025	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	96	0.15320534303633837	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	91	0.1452258980865291	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	90	0.14363000909656726	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	83	0.13245878616683424	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	82	0.13086289717687238	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	73	0.11649989626721566	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	67	0.1069245623274445	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	63	0.10054100636759708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015958889899618583	0.0
2	0.0	0.0	0.0	0.0015958889899618583	0.0
3	0.0	0.0	0.0	0.0015958889899618583	0.0
4	0.0	0.0	0.0	0.0015958889899618583	0.0
5	0.0	0.0	0.0	0.0015958889899618583	0.0
6	0.0	0.0	0.0	0.0015958889899618583	0.0
7	0.0	0.0	0.0	0.0031917779799237166	0.0
8	0.0	0.0	0.0	0.0031917779799237166	0.0
9	0.0	0.0	0.0	0.006383555959847433	0.0
10	0.0	0.0	0.0	0.006383555959847433	0.0
11	0.0	0.0	0.0	0.006383555959847433	0.0
12	0.0	0.0	0.0	0.00797944494980929	0.0
13	0.0	0.0	0.0	0.00797944494980929	0.0
14	0.0	0.0	0.0	0.00797944494980929	0.0
15	0.0	0.0	0.0	0.00797944494980929	0.0
16	0.0	0.0	0.0	0.00797944494980929	0.0
17	0.0	0.0	0.0	0.00797944494980929	0.0
18	0.0	0.0	0.0	0.00797944494980929	0.0
19	0.0	0.0	0.0	0.00797944494980929	0.0
20	0.0	0.0	0.0	0.00957533393977115	0.0
21	0.0	0.0	0.0	0.011171222929733007	0.0
22	0.0	0.0	0.0	0.012767111919694867	0.0
23	0.0	0.0	0.0	0.012767111919694867	0.0
24	0.0	0.0	0.0	0.012767111919694867	0.0
25	0.0	0.0	0.0	0.012767111919694867	0.0
26	0.0	0.0	0.0	0.014363000909656724	0.0
27	0.0	0.0	0.0	0.03670544676912274	0.0
28	0.0	0.0	0.0	0.08936978343786406	0.0
29	0.0	0.0	0.0	0.19789023475527043	0.0
30	0.0	0.0	0.0	0.3175819090024098	0.0
31	0.0	0.0	0.0	0.6495268189144763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACAT	45	0.0037861771	20.555555	5
GAAGAGG	50	0.0069627054	18.499998	6
AGAAGAG	50	0.0069627054	18.499998	5
AAGGGAA	50	0.0069627054	18.499998	5
TCTTATA	80	3.319171E-4	16.1875	37
CTCTTAT	120	3.203617E-5	13.875001	37
GTCTCTT	260	1.8189894E-12	13.519231	37
TCTCTTA	160	4.6860173E-4	10.40625	36
GCTGTCT	195	2.6977042E-4	9.487179	37
TGTCTCT	385	1.9153958E-9	9.129869	36
CTGTCTC	455	4.783942E-10	8.538462	37
CCTGTCT	275	9.7514375E-4	7.3999996	35
>>END_MODULE