FastQCFastQC Report
Fri 10 Feb 2017
ERR1632076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879482
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109100.5804790894512424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79900.42511713333780266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70450.37483732219835036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.17121738862090724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.019.4406781
ATACCGT752.0681467E-417.2666666
CTTATAC14150.016.08127237
TAGACAG2650.016.0566045
TTACACT1902.7284841E-1115.5789484
TAATACT1802.0190782E-1015.4166664
CGGACTA750.00410569614.824
TATACTG1904.5656634E-1014.6052645
GGACCTA1304.447291E-614.2307696
TCTATAC1702.4390829E-814.1470593
AATTACG1854.9039954E-914.016
TACTATA1854.9039954E-914.02
CCGCTTA800.00630058613.87525
TCTTATA22850.013.84463937
TATACAG1753.569221E-813.7428585
TACGTTA1753.569221E-813.74285819
GTATCAA47200.013.4046612
TAGACCC1254.8475835E-513.325
ATAGGAC2102.042725E-913.2142873
GCGTTAT850.00940763513.0588241
Produced by FastQC (version 0.11.5)