FastQCFastQC Report
Fri 10 Feb 2017
ERR1632075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1321.2605042016806722TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC680.6493506493506493No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC480.4583651642475172No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT390.37242169595110775No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC280.267379679144385RNA PCR Primer, Index 3 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG280.267379679144385No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG220.21008403361344538No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)