##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632075.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10472
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33680290297937	33.0	31.0	34.0	31.0	34.0
2	32.49828113063407	34.0	31.0	34.0	31.0	34.0
3	32.544117647058826	34.0	31.0	34.0	31.0	34.0
4	36.140756302521005	37.0	35.0	37.0	35.0	37.0
5	35.94623758594347	37.0	35.0	37.0	35.0	37.0
6	35.98004201680672	37.0	35.0	37.0	35.0	37.0
7	35.97068372803667	37.0	35.0	37.0	35.0	37.0
8	35.913388082505726	37.0	35.0	37.0	35.0	37.0
9	37.59883498854087	39.0	37.0	39.0	35.0	39.0
10	37.480901451489686	39.0	37.0	39.0	35.0	39.0
11	37.648491214667686	39.0	37.0	39.0	35.0	39.0
12	37.58985867074102	39.0	37.0	39.0	35.0	39.0
13	37.6801948051948	39.0	37.0	39.0	35.0	39.0
14	38.93716577540107	40.0	38.0	41.0	36.0	41.0
15	38.94805194805195	40.0	38.0	41.0	36.0	41.0
16	38.80127960275019	40.0	38.0	41.0	36.0	41.0
17	38.8320282658518	40.0	38.0	41.0	35.0	41.0
18	38.86535523300229	40.0	38.0	41.0	36.0	41.0
19	38.92007257448434	40.0	38.0	41.0	36.0	41.0
20	38.83212375859435	40.0	38.0	41.0	35.0	41.0
21	38.80920550038197	40.0	38.0	41.0	35.0	41.0
22	38.80452635599694	40.0	38.0	41.0	35.0	41.0
23	38.73071046600458	40.0	38.0	41.0	35.0	41.0
24	38.7014896867838	40.0	38.0	41.0	35.0	41.0
25	38.62901069518717	40.0	38.0	41.0	35.0	41.0
26	38.55882352941177	40.0	38.0	41.0	35.0	41.0
27	38.478227654698244	40.0	38.0	41.0	34.0	41.0
28	38.45874713521772	40.0	38.0	41.0	34.0	41.0
29	38.289056531703594	40.0	38.0	41.0	34.0	41.0
30	38.297746371275785	40.0	38.0	41.0	34.0	41.0
31	38.209606569900686	40.0	38.0	41.0	34.0	41.0
32	38.1707410236822	40.0	38.0	41.0	34.0	41.0
33	37.86946142093201	40.0	37.0	41.0	33.0	41.0
34	37.92866692131398	40.0	38.0	41.0	33.0	41.0
35	37.99856760886173	40.0	38.0	41.0	33.0	41.0
36	37.94566462948816	40.0	37.0	41.0	33.0	41.0
37	37.93812070282659	40.0	37.0	41.0	33.0	41.0
38	37.85246371275783	40.0	37.0	41.0	33.0	41.0
39	37.584606569900686	40.0	37.0	41.0	33.0	41.0
40	37.58384262796027	40.0	37.0	41.0	33.0	41.0
41	37.63044308632544	40.0	37.0	41.0	33.0	41.0
42	37.511172650878535	40.0	37.0	41.0	33.0	41.0
43	36.66348357524828	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	4.0
23	8.0
24	15.0
25	11.0
26	27.0
27	42.0
28	51.0
29	67.0
30	79.0
31	148.0
32	196.0
33	280.0
34	341.0
35	522.0
36	840.0
37	1477.0
38	3342.0
39	3019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17914438502674	17.54201680672269	13.59816653934301	26.680672268907564
2	20.01527883880825	19.948433919022154	31.493506493506494	28.542780748663098
3	19.938884644766997	20.645530939648587	29.612299465240643	29.803284950343773
4	16.69213139801375	15.269289533995417	29.917876241405654	38.12070282658518
5	17.09320091673033	32.61077158135981	32.78265851795263	17.51336898395722
6	37.85332314744079	31.312070282658517	14.35255920550038	16.482047364400305
7	30.672268907563026	28.84835752482811	18.84071810542399	21.638655462184875
8	29.364018334606566	30.815508021390375	18.487394957983195	21.333078686019864
9	26.938502673796794	13.216195569136746	17.914438502673796	41.93086325439266
10	19.11764705882353	24.178762414056532	30.414438502673796	26.289152024446143
11	37.7864782276547	18.97440794499618	19.17494270435447	24.06417112299465
12	23.634453781512605	24.216959511077157	25.773491214667686	26.375095492742552
13	34.090909090909086	15.947288006111535	21.543162719633308	28.418640183346067
14	25.152788388082502	18.74522536287242	22.202062643239113	33.89992360580596
15	27.568754774637128	25.286478227654698	18.955309396485866	28.189457601222305
16	29.49770817417876	23.137891520244462	20.091673032849503	27.27272727272727
17	25.67799847211612	25.25783040488923	22.163865546218485	26.900305576776162
18	27.234530175706645	21.361726508785335	23.128342245989305	28.27540106951872
19	28.590527119938887	23.47211611917494	22.345301757066462	25.59205500381971
20	27.396867838044308	22.478991596638657	22.78456837280367	27.339572192513366
21	28.924751718869366	23.23338426279603	20.760122230710465	27.081741787624143
22	28.49503437738732	23.347975553857907	22.421695951107715	25.735294117647058
23	27.7501909854851	23.81588999236058	21.1038961038961	27.330022918258212
24	29.440412528647826	22.135217723453017	21.419022154316274	27.00534759358289
25	27.912528647822764	23.605805958747137	21.772345301757067	26.709320091673032
26	28.42818945760122	23.42436974789916	21.29488158899924	26.85255920550038
27	27.12948815889992	22.813216195569137	22.708174178762413	27.349121466768526
28	26.566080977845687	23.319327731092436	22.956455309396485	27.158135981665392
29	27.377769289533994	22.956455309396485	22.450343773873186	27.215431627196335
30	26.289152024446143	22.899159663865547	24.092818945760122	26.71886936592819
31	27.072192513368986	23.262032085561497	21.73414820473644	27.931627196333082
32	27.263177998472116	23.10924369747899	23.22383498854087	26.40374331550802
33	26.193659281894575	22.593582887700535	23.97822765469824	27.234530175706645
34	27.310924369747898	23.634453781512605	22.459893048128343	26.594728800611158
35	26.632925897631782	20.922459893048128	25.67799847211612	26.766615737203974
36	26.136363636363637	23.214285714285715	23.06149732620321	27.587853323147442
37	25.13368983957219	21.218487394957982	24.818563789152023	28.8292589763178
38	25.391520244461418	21.57181054239878	24.68487394957983	28.35179526355997
39	25.802139037433154	21.151642475171887	25.954927425515663	27.091291061879296
40	23.64400305576776	20.21581359816654	28.58097784568373	27.55920550038197
41	24.933155080213904	20.091673032849503	27.492360580595875	27.482811306340714
42	23.06149732620321	19.385026737967912	29.85103132161956	27.70244461420932
43	22.345301757066462	18.821619556913674	29.669595110771578	29.163483575248282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	2.0
9	1.0
10	1.5
11	2.0
12	2.0
13	1.5
14	1.0
15	3.5
16	6.0
17	6.5
18	7.0
19	7.0
20	9.0
21	11.0
22	16.0
23	21.0
24	23.5
25	26.0
26	26.0
27	33.5
28	41.0
29	56.5
30	72.0
31	102.0
32	132.0
33	132.0
34	159.5
35	187.0
36	214.5
37	242.0
38	280.5
39	319.0
40	319.0
41	364.5
42	410.0
43	470.5
44	531.0
45	560.0
46	589.0
47	589.0
48	667.0
49	745.0
50	833.5
51	922.0
52	916.0
53	910.0
54	910.0
55	1006.5
56	1103.0
57	1046.0
58	989.0
59	896.0
60	803.0
61	803.0
62	750.5
63	698.0
64	612.5
65	527.0
66	467.0
67	407.0
68	407.0
69	344.0
70	281.0
71	236.0
72	191.0
73	158.0
74	125.0
75	125.0
76	95.5
77	66.0
78	59.0
79	52.0
80	38.0
81	24.0
82	24.0
83	18.5
84	13.0
85	12.0
86	11.0
87	7.0
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	10472.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.76165011459129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.04550133827465	90.02100840336135
2	2.1000617665225447	3.896103896103896
3	0.5250154416306362	1.461038961038961
4	0.12353304508956146	0.4583651642475172
5	0.08235536339304096	0.38197097020626436
6	0.04117768169652048	0.2291825821237586
7	0.01029442042413012	0.06684491978609625
8	0.0	0.0
9	0.0	0.0
>10	0.051472102120650606	1.5756302521008403
>50	0.01029442042413012	0.6493506493506493
>100	0.01029442042413012	1.2605042016806722
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	132	1.2605042016806722	TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	68	0.6493506493506493	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	48	0.4583651642475172	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	39	0.37242169595110775	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	28	0.267379679144385	RNA PCR Primer, Index 3 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	28	0.267379679144385	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	22	0.21008403361344538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.07639419404125286	0.0
29	0.0	0.0	0.0	0.20053475935828877	0.0
30	0.0	0.0	0.0	0.3246753246753247	0.0
31	0.0	0.0	0.0	0.6684491978609626	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE