FastQCFastQC Report
Fri 10 Feb 2017
ERR1632074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349174
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.1589069179209373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25420.10820824681356085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25170.10714404296999712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.023.8471781
CTTATAC28850.018.91681137
GTATCAA20950.016.601432
TACACCG1251.6597551E-716.2799995
CGAATTA803.3844457E-416.187515
CTAGACG700.002593573415.8571434
ATTCGCG700.002593573415.85714330
TATACAC9600.015.22395937
TCTTATA49250.015.10050937
TCGCTAA750.00410610314.79999914
CTTACAC2550.014.5098043
TCGTTTA1956.730261E-1014.2307730
TTACACT1854.9058144E-914.04
ATTCGTA2405.456968E-1213.87500124
GTTCTAA1601.7856837E-713.8751
TAAGACG800.006301202313.8754
GCTAGAC1102.458071E-413.4545453
TAAACGA1951.0273652E-813.28205216
CGTTTAT2102.044544E-913.21428531
TTTAGCG850.009408548513.05882426
Produced by FastQC (version 0.11.5)