FastQCFastQC Report
Fri 10 Feb 2017
ERR1632073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3531727
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49270.13950681918506158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.11793097258083651No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA38010.1076244001872172No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG37300.10561405227527496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.026.3668211
GTATCAA31150.018.0545752
ACTATCG1005.884731E-616.6500028
TCTTATA37100.015.55795137
CTTATAC21800.015.27522937
TATGCCG1904.5656634E-1014.60526433
TATACCG1152.2122998E-514.478264
CGAATTA2450.014.34693815
CGTATGC2101.364242E-1014.09523831
CTCTTAT59300.014.069983537
TCGTATG2009.858923E-1013.87500130
TACGTTA1601.7865386E-713.87500119
ATACCGT2700.013.7037046
ATCAACG41500.013.3734944
CGTATCG1409.571426E-613.21428510
TCAACGC42000.013.2142855
CGTAACA850.00940977613.0588242
TATCAAC44050.012.9353013
CAACGCA42900.012.8076926
GTACTAT2900.012.758621
Produced by FastQC (version 0.11.5)