##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3531727
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90394472732462	31.0	31.0	34.0	30.0	34.0
2	32.09217303602458	33.0	31.0	34.0	30.0	34.0
3	32.11190729068243	34.0	31.0	34.0	30.0	34.0
4	35.85143189153635	37.0	35.0	37.0	35.0	37.0
5	35.39637152022226	37.0	35.0	37.0	33.0	37.0
6	35.38539785209899	37.0	35.0	37.0	33.0	37.0
7	35.38456398243692	37.0	35.0	37.0	33.0	37.0
8	35.334502355363256	37.0	35.0	37.0	32.0	37.0
9	36.986709051973726	39.0	37.0	39.0	33.0	39.0
10	36.80291709976451	38.0	37.0	39.0	33.0	39.0
11	36.94484539716688	39.0	37.0	39.0	33.0	39.0
12	36.89523029384774	39.0	37.0	39.0	33.0	39.0
13	36.95398851609991	39.0	37.0	39.0	33.0	39.0
14	38.102931511977005	40.0	38.0	41.0	33.0	41.0
15	38.0799993884012	40.0	38.0	41.0	33.0	41.0
16	37.94810470911257	39.0	37.0	41.0	33.0	41.0
17	37.986810135664506	39.0	37.0	41.0	33.0	41.0
18	37.989785733721774	39.0	37.0	41.0	33.0	41.0
19	38.064454019237616	40.0	37.0	41.0	34.0	41.0
20	38.033653507193506	40.0	37.0	41.0	33.0	41.0
21	37.99426569494188	40.0	37.0	41.0	33.0	41.0
22	37.97755913749845	40.0	37.0	41.0	33.0	41.0
23	37.93012115602367	39.0	37.0	40.0	33.0	41.0
24	37.85882827296674	39.0	37.0	41.0	33.0	41.0
25	37.76690468997179	39.0	37.0	40.0	33.0	41.0
26	37.63965872786883	39.0	37.0	40.0	33.0	41.0
27	37.54613847559565	39.0	37.0	40.0	32.0	41.0
28	37.470952879426974	39.0	37.0	40.0	32.0	41.0
29	37.31812594801354	39.0	36.0	40.0	32.0	41.0
30	37.256646394242814	39.0	36.0	40.0	32.0	41.0
31	37.1519168950488	39.0	36.0	40.0	31.0	41.0
32	37.06047268092919	39.0	36.0	40.0	31.0	41.0
33	36.91344716055346	39.0	36.0	40.0	31.0	41.0
34	36.905691747974856	39.0	36.0	40.0	31.0	41.0
35	36.86511896304556	39.0	36.0	40.0	31.0	41.0
36	36.836489060451164	39.0	36.0	40.0	31.0	41.0
37	36.81008554738234	39.0	36.0	40.0	31.0	41.0
38	36.72314904294698	39.0	35.0	40.0	30.0	41.0
39	36.533540106582414	38.0	35.0	40.0	30.0	41.0
40	36.54091921600962	38.0	35.0	40.0	30.0	41.0
41	36.53575941741816	38.0	35.0	40.0	30.0	41.0
42	36.41991439315666	38.0	35.0	40.0	30.0	41.0
43	35.53626030551059	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	7.0
11	2.0
12	3.0
13	9.0
14	15.0
15	38.0
16	62.0
17	118.0
18	244.0
19	433.0
20	728.0
21	1497.0
22	2452.0
23	4056.0
24	6122.0
25	9607.0
26	14056.0
27	20429.0
28	29354.0
29	40144.0
30	54572.0
31	73625.0
32	98731.0
33	135845.0
34	192741.0
35	283428.0
36	436804.0
37	683579.0
38	943247.0
39	499776.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.02267049519966	18.95956284276786	12.9878102129638	26.029956449068685
2	19.627054979051326	20.799908939734017	34.06217411481692	25.51086196639774
3	20.983954875334362	22.115412657886637	29.496758951074074	27.403873515704923
4	16.150936921228624	16.038272493881887	32.91171712875882	34.89907345613067
5	16.05356246391638	35.01805207480646	33.12022701641435	15.80815844486281
6	35.23650044298441	33.41648434321226	15.530390655902906	15.816624557900427
7	29.344991841102104	29.48979352028059	20.960113847984285	20.205100790633026
8	26.534298942132278	33.08565469528081	20.13949549328133	20.240550869305586
9	26.29198123184493	14.684657109680336	19.869570892653936	39.1537907658208
10	17.66407765945669	26.671965301961336	32.805423522259794	22.858533516322186
11	35.79990752399605	21.435065620870468	21.173069152853547	21.59195770227993
12	21.803129177311835	25.11771153319608	28.287973560810336	24.791185728681747
13	30.52096042531034	19.51798652613863	24.274979351461763	25.686073697089274
14	23.36491467205704	20.90900570740604	23.98973646604055	31.73634315449637
15	26.0547035487171	27.24913335600402	21.821731974187134	24.87443112109175
16	25.825042535847192	25.86578747451318	23.362677806070515	24.946492183569116
17	24.52715059799356	26.076251080562002	24.651707224256008	24.74489109718843
18	23.869370424157925	25.003206646493343	26.0460109176049	25.08141201174383
19	25.220239276705136	25.15409599892631	25.86836411761158	23.757300606756978
20	25.337717213136802	24.555040635926844	25.886145786466507	24.221096364469847
21	25.191471481232835	25.303626242911754	24.725835264163962	24.779067011691446
22	25.36634343481249	24.692820254793194	24.80571120021451	25.135125110179807
23	24.724589414753744	25.177285786811947	25.035740304955624	25.062384493478685
24	25.731320682487635	25.133029817989893	24.648026305544	24.487623193978468
25	25.45414750347351	24.49866595011449	25.079203460516624	24.96798308589537
26	25.228365612630878	24.948672420036996	24.87876327926819	24.944198688063942
27	25.145573256370042	24.60413842859315	24.842463757815935	25.40782455722087
28	24.561071679662668	24.88646489380408	25.496109976790393	25.056353449742858
29	24.64847934169317	25.241277142882225	25.324890627163427	24.785352888261183
30	24.38438758148634	25.857802712384053	25.109755085826286	24.648054620303324
31	25.219389833925444	24.867607264094875	25.252518102333504	24.66048479964618
32	24.240407030328225	24.824993551313565	25.450976250429324	25.483623167928894
33	24.492068611192202	24.515003566243937	25.511994556770667	25.480933265793194
34	24.956090886979656	24.25620666603053	25.459045956836412	25.3286564901534
35	24.887257707065128	24.252214284965966	25.655550386538938	25.20497762142997
36	24.454126833699206	24.416751351392676	25.903049697782414	25.226072117125703
37	25.14775349283792	23.077661438723887	26.112437342976964	25.662147725461224
38	24.10316539188901	23.61332005559886	27.21178618845681	25.071728364055318
39	23.970652318256764	23.192959138687673	27.578094229820145	25.258294313235425
40	23.566600702715697	22.907687938507138	28.273872810667417	25.251838548109752
41	22.903978705035808	23.32264073638761	28.546062592040666	25.227317966535917
42	23.378052720382975	22.958484616732832	28.08170053914133	25.581762123742863
43	22.522550582193922	23.13369634742436	28.435804919236396	25.907948151145316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	257.0
2	266.0
3	619.0
4	972.0
5	972.0
6	1278.5
7	1585.0
8	1549.5
9	1514.0
10	2308.0
11	3102.0
12	3102.0
13	5092.5
14	7083.0
15	10044.0
16	13005.0
17	13354.5
18	13704.0
19	13704.0
20	17069.0
21	20434.0
22	22794.0
23	25154.0
24	29671.5
25	34189.0
26	34189.0
27	39085.0
28	43981.0
29	58658.5
30	73336.0
31	81727.0
32	90118.0
33	90118.0
34	102034.0
35	113950.0
36	126099.0
37	138248.0
38	159549.5
39	180851.0
40	180851.0
41	197188.5
42	213526.0
43	210461.5
44	207397.0
45	221885.5
46	236374.0
47	236374.0
48	247161.5
49	257949.0
50	271322.5
51	284696.0
52	297958.0
53	311220.0
54	311220.0
55	279904.5
56	248589.0
57	235489.5
58	222390.0
59	205279.5
60	188169.0
61	188169.0
62	175094.5
63	162020.0
64	145448.0
65	128876.0
66	113453.5
67	98031.0
68	98031.0
69	84528.5
70	71026.0
71	61735.5
72	52445.0
73	43057.5
74	33670.0
75	33670.0
76	27221.0
77	20772.0
78	17342.5
79	13913.0
80	11223.0
81	8533.0
82	8533.0
83	6695.0
84	4857.0
85	3862.5
86	2868.0
87	2217.0
88	1566.0
89	1566.0
90	1210.5
91	855.0
92	510.5
93	166.0
94	111.5
95	57.0
96	57.0
97	37.5
98	18.0
99	11.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3531727.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.70865583242942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.04162660571126	45.67471454796119
2	8.60568451429625	9.243994955616094
3	2.4423315782748287	3.9352303849871073
4	1.056258778917869	2.2692095690752794
5	0.6200943770286539	1.6652217739728348
6	0.40348929460497124	1.3002520593604872
7	0.2777340052821421	1.0441704071862492
8	0.2190914154812929	0.9413684343939646
9	0.1541270715296918	0.7450162055323604
>10	0.9667000557574147	9.817005758812307
>50	0.10081945730788405	3.79782075813253
>100	0.09499227016985042	10.622057268998638
>500	0.012284886796382807	4.493668552027324
>1k	0.004765688841475178	4.450269323943687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4927	0.13950681918506158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4165	0.11793097258083651	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3801	0.1076244001872172	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3730	0.10561405227527496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.662951864626003E-5	0.0	0.0	0.0
6	0.0	5.662951864626003E-5	0.0	0.0	0.0
7	0.0	5.662951864626003E-5	0.0	0.0	0.0
8	0.0	5.662951864626003E-5	0.0	2.8314759323130015E-5	0.0
9	2.8314759323130015E-5	5.662951864626003E-5	0.0	2.8314759323130015E-5	0.0
10	2.8314759323130015E-5	5.662951864626003E-5	0.0	2.8314759323130015E-5	2.8314759323130015E-5
11	2.8314759323130015E-5	1.4157379661565008E-4	0.0	2.8314759323130015E-5	2.8314759323130015E-5
12	2.8314759323130015E-5	1.4157379661565008E-4	0.0	8.494427796939005E-5	2.8314759323130015E-5
13	2.8314759323130015E-5	1.4157379661565008E-4	0.0	8.494427796939005E-5	2.8314759323130015E-5
14	2.8314759323130015E-5	1.4157379661565008E-4	0.0	8.494427796939005E-5	2.8314759323130015E-5
15	2.8314759323130015E-5	1.4157379661565008E-4	0.0	1.698885559387801E-4	2.8314759323130015E-5
16	2.8314759323130015E-5	1.698885559387801E-4	0.0	4.5303614917008024E-4	5.662951864626003E-5
17	2.8314759323130015E-5	1.698885559387801E-4	0.0	8.211280203707705E-4	5.662951864626003E-5
18	2.8314759323130015E-5	1.698885559387801E-4	0.0	9.060722983401605E-4	5.662951864626003E-5
19	2.8314759323130015E-5	1.698885559387801E-4	0.0	0.0010193313356326806	5.662951864626003E-5
20	2.8314759323130015E-5	1.698885559387801E-4	0.0	0.0010759608542789405	8.494427796939005E-5
21	2.8314759323130015E-5	1.698885559387801E-4	0.0	0.0013591084475102408	1.1325903729252006E-4
22	2.8314759323130015E-5	1.698885559387801E-4	0.0	0.001925403633972841	1.1325903729252006E-4
23	2.8314759323130015E-5	1.982033152619101E-4	0.0	0.0030013644882517817	1.1325903729252006E-4
24	2.8314759323130015E-5	1.982033152619101E-4	0.0	0.004473731973054542	1.1325903729252006E-4
25	2.8314759323130015E-5	1.982033152619101E-4	0.0	0.006512394644319903	1.1325903729252006E-4
26	2.8314759323130015E-5	1.982033152619101E-4	0.0	0.011524107044513916	1.4157379661565008E-4
27	2.8314759323130015E-5	1.982033152619101E-4	0.0	0.03411928498437167	1.4157379661565008E-4
28	2.8314759323130015E-5	3.114623525544302E-4	0.0	0.08919149186785955	1.4157379661565008E-4
29	2.8314759323130015E-5	3.680918712006902E-4	0.0	0.1605446853621472	1.4157379661565008E-4
30	2.8314759323130015E-5	3.680918712006902E-4	0.0	0.25483283390817013	1.4157379661565008E-4
31	2.8314759323130015E-5	3.680918712006902E-4	0.0	0.5333651213698001	1.4157379661565008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2140	0.0	26.366821	1
GTATCAA	3115	0.0	18.054575	2
ACTATCG	100	5.884731E-6	16.650002	8
TCTTATA	3710	0.0	15.557951	37
CTTATAC	2180	0.0	15.275229	37
TATGCCG	190	4.5656634E-10	14.605264	33
TATACCG	115	2.2122998E-5	14.47826	4
CGAATTA	245	0.0	14.346938	15
CGTATGC	210	1.364242E-10	14.095238	31
CTCTTAT	5930	0.0	14.0699835	37
TCGTATG	200	9.858923E-10	13.875001	30
TACGTTA	160	1.7865386E-7	13.875001	19
ATACCGT	270	0.0	13.703704	6
ATCAACG	4150	0.0	13.373494	4
CGTATCG	140	9.571426E-6	13.214285	10
TCAACGC	4200	0.0	13.214285	5
CGTAACA	85	0.009409776	13.058824	2
TATCAAC	4405	0.0	12.935301	3
CAACGCA	4290	0.0	12.807692	6
GTACTAT	290	0.0	12.75862	1
>>END_MODULE