FastQCFastQC Report
Fri 10 Feb 2017
ERR1632072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572841
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10450.18242409324751546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.1752667843258426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6040.10543938021196107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT450.003823500420.5555556
GGTATCA6550.018.9236641
TTTAGGA609.2291046E-418.52
GTACTAT650.001578625617.0769251
TTAAGCT1403.4620825E-815.8571434
TTTAAGC957.049797E-515.5789483
ACAATGC957.049797E-515.5789488
CGTAGCC855.358318E-415.2352943
CTTATAC4950.014.94949537
ATCTCGC1252.9522926E-614.79999911
TATACAC2151.9645086E-1013.76744237
TAGAGTG950.001243739613.6315795
ATAGCAC950.001243739613.6315793
GTATCAA9200.013.4728262
TAATCTC1254.8360947E-513.325
TCTTATA7300.013.17808237
TCTTAAG850.009397197513.0588242
TTAACAC850.009397197513.0588243
TGTACAC850.009397197513.0588243
TAATGGT850.009397197513.0588244