##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632072.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572841
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05368679965296	33.0	31.0	34.0	30.0	34.0
2	32.234586909805685	33.0	31.0	34.0	30.0	34.0
3	32.24902547129133	34.0	31.0	34.0	30.0	34.0
4	35.94068511157546	37.0	35.0	37.0	35.0	37.0
5	35.60691535696642	37.0	35.0	37.0	33.0	37.0
6	35.60519236576991	37.0	35.0	37.0	33.0	37.0
7	35.59601704486934	37.0	35.0	37.0	33.0	37.0
8	35.560743731681214	37.0	35.0	37.0	33.0	37.0
9	37.2425070831173	39.0	37.0	39.0	34.0	39.0
10	37.072704293163376	39.0	37.0	39.0	33.0	39.0
11	37.2226376952767	39.0	37.0	39.0	34.0	39.0
12	37.1573996972982	39.0	37.0	39.0	34.0	39.0
13	37.21803083229029	39.0	37.0	39.0	34.0	39.0
14	38.40213951166205	40.0	38.0	41.0	34.0	41.0
15	38.38802913897574	40.0	38.0	41.0	34.0	41.0
16	38.25615834062157	40.0	38.0	41.0	34.0	41.0
17	38.29458959816075	40.0	38.0	41.0	34.0	41.0
18	38.301099257909264	40.0	38.0	41.0	34.0	41.0
19	38.36622727772628	40.0	38.0	41.0	34.0	41.0
20	38.33828584197011	40.0	38.0	41.0	34.0	41.0
21	38.30643232589846	40.0	38.0	41.0	34.0	41.0
22	38.28146379187244	40.0	38.0	41.0	34.0	41.0
23	38.22510783969723	40.0	38.0	41.0	34.0	41.0
24	38.15111173955775	40.0	38.0	41.0	34.0	41.0
25	38.065875173041036	40.0	38.0	41.0	34.0	41.0
26	37.94073922781365	40.0	37.0	41.0	33.0	41.0
27	37.862317815938454	40.0	37.0	41.0	33.0	41.0
28	37.764103826367176	40.0	37.0	41.0	33.0	41.0
29	37.59870539992773	39.0	37.0	41.0	33.0	41.0
30	37.53195214727996	39.0	37.0	40.0	32.0	41.0
31	37.432612190817345	39.0	37.0	40.0	32.0	41.0
32	37.33381514242172	39.0	36.0	40.0	32.0	41.0
33	37.174070990030394	39.0	36.0	40.0	31.0	41.0
34	37.15962893717454	39.0	36.0	40.0	31.0	41.0
35	37.11562370710197	39.0	36.0	40.0	31.0	41.0
36	37.088556859582326	39.0	36.0	40.0	31.0	41.0
37	37.053372576334446	39.0	36.0	40.0	31.0	41.0
38	36.95826590624623	39.0	36.0	40.0	31.0	41.0
39	36.75853159951889	39.0	35.0	40.0	31.0	41.0
40	36.75045955160333	39.0	35.0	40.0	31.0	41.0
41	36.732997463519546	39.0	35.0	40.0	31.0	41.0
42	36.6126691350654	39.0	35.0	40.0	30.0	41.0
43	35.68515521759092	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	0.0
14	0.0
15	3.0
16	9.0
17	10.0
18	27.0
19	64.0
20	112.0
21	197.0
22	361.0
23	552.0
24	949.0
25	1366.0
26	2040.0
27	2927.0
28	4306.0
29	6014.0
30	7871.0
31	10630.0
32	14239.0
33	18856.0
34	27158.0
35	39407.0
36	62432.0
37	104648.0
38	167547.0
39	101111.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.51843007047331	18.493264274030665	12.643997199921095	26.344308455574932
2	20.16458319149642	20.318901754588097	33.15876482304863	26.357750230866856
3	21.319353887029735	21.39895712771956	28.970866261318584	28.310822723932123
4	16.45378036837447	15.720243488158145	32.21574573049066	35.610230412976726
5	16.814788047643237	34.030210826389876	32.643962286219036	16.51103883974785
6	35.92515200553033	32.7322939524231	15.19199917603663	16.15055486600994
7	30.189878168636675	28.849715715181002	20.309998760563577	20.65040735561875
8	27.715544103861276	32.0235458006672	19.45653331378166	20.804376781689857
9	26.865046321754203	13.96425884320431	19.229943387432115	39.94075144760937
10	18.262484703434286	25.87943251268677	32.10297447284674	23.755108311032206
11	36.80166049566983	20.73245455545256	20.289050539329413	22.1768344095482
12	22.24805836174436	24.568248431938354	27.92258235705894	25.261110849258344
13	31.713512126401568	18.809756983176833	23.369137334792725	26.10759355562887
14	23.853914087853347	20.16877283574325	23.593457870508573	32.38385520589483
15	26.715964813971066	26.126970660270477	21.251795873549554	25.905268652208903
16	26.185800248236426	25.141182282692753	23.001670620643424	25.6713468484274
17	24.848081753924735	25.456453012266927	24.395949312287353	25.29951592152098
18	24.85070028157901	24.216492883714675	25.338968404845325	25.593838429860995
19	25.779579324803915	24.436100069652834	25.259190595645215	24.525130009898035
20	25.982951639285595	23.934215602584313	25.14327710481617	24.93955565331392
21	26.15455248489546	24.146490911090513	24.31268013288155	25.38627647113248
22	26.18161060398959	24.0918509673714	24.32297967498835	25.403558753650664
23	25.8516761195515	24.192227860785103	24.28998622654454	25.666109793118856
24	25.939658648734987	24.42248372585063	24.290160795054824	25.347696830359556
25	26.102181931810048	23.825459420676943	24.247915215565925	25.824443431947085
26	25.763693590368007	24.317742619679805	24.27183110147493	25.646732688477258
27	25.711672174303168	24.096040611618232	24.224523035187776	25.967764178890828
28	25.241733744616745	24.323503380519202	24.80112282465815	25.6336400502059
29	25.15619517457724	24.346022718345928	25.025792497394566	25.471989609682268
30	25.181682177078805	24.80548703741527	24.86117439219609	25.151656393309835
31	25.654239134419498	24.228538110924323	24.557425184300705	25.559797570355475
32	24.95771077838353	24.323503380519202	24.990180521296484	25.728605319800785
33	25.034695491419086	24.076488938466344	25.062277316044067	25.826538254070503
34	25.65982532674861	23.82580855769751	24.6909700946685	25.823396020885376
35	25.12896248697282	23.875211446108082	25.346998556318418	25.648827510600675
36	24.603511271015865	23.766804401221282	25.506379606208355	26.123304721554497
37	25.141182282692753	22.506245188455434	26.135699085784715	26.2168734430671
38	24.310759879268417	22.795156072976617	27.031933817586378	25.86215023016858
39	24.084519089939445	22.62372979587704	27.39817855216369	25.893572562019827
40	23.75720313315562	22.3171874918171	28.14760116681592	25.778008208211357
41	23.000972346602286	22.490708591040097	28.507386866512697	26.00093219584492
42	23.11461644679763	22.590736347433232	28.212191515621264	26.082455690147878
43	22.477965089789315	22.51060940121255	28.688414411677936	26.323011097320197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	22.5
2	27.0
3	92.5
4	158.0
5	158.0
6	201.0
7	244.0
8	251.0
9	258.0
10	365.5
11	473.0
12	473.0
13	915.0
14	1357.0
15	1941.0
16	2525.0
17	2368.5
18	2212.0
19	2212.0
20	2940.0
21	3668.0
22	3805.0
23	3942.0
24	4686.0
25	5430.0
26	5430.0
27	6007.0
28	6584.0
29	8079.5
30	9575.0
31	11295.5
32	13016.0
33	13016.0
34	14877.5
35	16739.0
36	18929.0
37	21119.0
38	23166.0
39	25213.0
40	25213.0
41	27469.5
42	29726.0
43	30688.5
44	31651.0
45	33771.5
46	35892.0
47	35892.0
48	37672.5
49	39453.0
50	41060.5
51	42668.0
52	44422.0
53	46176.0
54	46176.0
55	43804.0
56	41432.0
57	40559.0
58	39686.0
59	37597.5
60	35509.0
61	35509.0
62	33236.0
63	30963.0
64	27983.5
65	25004.0
66	22192.0
67	19380.0
68	19380.0
69	16890.5
70	14401.0
71	12512.5
72	10624.0
73	8930.0
74	7236.0
75	7236.0
76	5796.0
77	4356.0
78	3650.0
79	2944.0
80	2248.5
81	1553.0
82	1553.0
83	1207.5
84	862.0
85	646.0
86	430.0
87	326.5
88	223.0
89	223.0
90	152.0
91	81.0
92	53.0
93	25.0
94	15.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	572841.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.52575577825242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44078484232449	67.40167843552548
2	5.857202595831142	8.730249004013155
3	1.4112783534404798	3.155297577109182
4	0.6179540065878554	1.8421395750863636
5	0.3587736786560388	1.3368939777592581
6	0.230242665122015	1.029540517837035
7	0.15684178876059746	0.818212699149758
8	0.11899103590349365	0.7094317505236026
9	0.10069643961367698	0.675403043974959
>10	0.6315787218259218	9.212142579943873
>50	0.058269140907316476	2.860304894167861
>100	0.01668186355290537	1.764158736248943
>500	2.3495582468787546E-4	0.10576197287257365
>1k	4.699116493757509E-4	0.35878523578791954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1045	0.18242409324751546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1004	0.1752667843258426	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.10543938021196107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.745685102847038E-4	0.0	0.0	0.0
4	0.0	1.745685102847038E-4	0.0	0.0	0.0
5	0.0	3.491370205694076E-4	0.0	0.0	0.0
6	0.0	3.491370205694076E-4	0.0	0.0	0.0
7	0.0	3.491370205694076E-4	0.0	0.0	0.0
8	0.0	3.491370205694076E-4	0.0	0.0	0.0
9	0.0	3.491370205694076E-4	0.0	1.745685102847038E-4	1.745685102847038E-4
10	0.0	3.491370205694076E-4	0.0	1.745685102847038E-4	1.745685102847038E-4
11	0.0	3.491370205694076E-4	0.0	3.491370205694076E-4	1.745685102847038E-4
12	0.0	3.491370205694076E-4	0.0	5.237055308541114E-4	1.745685102847038E-4
13	0.0	3.491370205694076E-4	0.0	5.237055308541114E-4	1.745685102847038E-4
14	0.0	3.491370205694076E-4	0.0	5.237055308541114E-4	1.745685102847038E-4
15	0.0	3.491370205694076E-4	0.0	5.237055308541114E-4	1.745685102847038E-4
16	0.0	3.491370205694076E-4	0.0	8.728425514235189E-4	1.745685102847038E-4
17	0.0	3.491370205694076E-4	0.0	0.0019202536131317416	1.745685102847038E-4
18	0.0	3.491370205694076E-4	0.0	0.0019202536131317416	1.745685102847038E-4
19	0.0	3.491370205694076E-4	0.0	0.002618527654270557	1.745685102847038E-4
20	0.0	3.491370205694076E-4	0.0	0.003142233185124668	1.745685102847038E-4
21	0.0	3.491370205694076E-4	0.0	0.004538781267402299	1.745685102847038E-4
22	0.0	3.491370205694076E-4	0.0	0.006284466370249336	1.745685102847038E-4
23	0.0	3.491370205694076E-4	0.0	0.007506445942242263	1.745685102847038E-4
24	0.0	3.491370205694076E-4	0.0	0.009950405086228116	1.745685102847038E-4
25	0.0	3.491370205694076E-4	0.0	0.01204522720964456	1.745685102847038E-4
26	0.0	3.491370205694076E-4	0.0	0.015885734435908044	3.491370205694076E-4
27	0.0	3.491370205694076E-4	0.0	0.03962705183462776	3.491370205694076E-4
28	0.0	5.237055308541114E-4	0.0	0.10980359296907868	3.491370205694076E-4
29	0.0	5.237055308541114E-4	0.0	0.19499302598801413	3.491370205694076E-4
30	0.0	5.237055308541114E-4	0.0	0.3168418461667374	3.491370205694076E-4
31	0.0	5.237055308541114E-4	0.0	0.6225113076752536	3.491370205694076E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	45	0.0038235004	20.555555	6
GGTATCA	655	0.0	18.923664	1
TTTAGGA	60	9.2291046E-4	18.5	2
GTACTAT	65	0.0015786256	17.076925	1
TTAAGCT	140	3.4620825E-8	15.857143	4
TTTAAGC	95	7.049797E-5	15.578948	3
ACAATGC	95	7.049797E-5	15.578948	8
CGTAGCC	85	5.358318E-4	15.235294	3
CTTATAC	495	0.0	14.949495	37
ATCTCGC	125	2.9522926E-6	14.799999	11
TATACAC	215	1.9645086E-10	13.767442	37
TAGAGTG	95	0.0012437396	13.631579	5
ATAGCAC	95	0.0012437396	13.631579	3
GTATCAA	920	0.0	13.472826	2
TAATCTC	125	4.8360947E-5	13.32	5
TCTTATA	730	0.0	13.178082	37
TCTTAAG	85	0.0093971975	13.058824	2
TTAACAC	85	0.0093971975	13.058824	3
TGTACAC	85	0.0093971975	13.058824	3
TAATGGT	85	0.0093971975	13.058824	4
>>END_MODULE