FastQCFastQC Report
Fri 10 Feb 2017
ERR1632071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000341
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.22962169900064078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16790.16784276561692463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16310.16304440185896607No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG11320.11316141195852214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.030.3699421
TATAACT652.682269E-622.769234
TACTATA705.10083E-621.1428592
CATAATA450.00382546620.5555552
CTATACG500.0070342618.54
TAACGTT500.0070342618.518
ATATAAC609.2356704E-418.53
ATAACGT500.0070342618.517
GTATCAA15200.017.2828942
ATACACT752.0669289E-417.2666664
ATTTAGG650.001579740417.0769232
GTAAATC650.001579740417.07692316
AATCGAG650.001579740417.07692322
GTACTAT1201.0407712E-716.9583341
CTTATAC11150.016.92376737
GTGTTAG803.3820161E-416.18751
TCTAGAC803.3820161E-416.18753
TCTACAC700.002592177815.8571433
CCATACA700.002592177815.8571434
ACATATA700.002592177815.8571431
Produced by FastQC (version 0.11.5)