##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632071.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000341
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81786010970259	31.0	31.0	34.0	30.0	34.0
2	31.98921767677222	33.0	31.0	34.0	30.0	34.0
3	32.01248674202097	33.0	31.0	34.0	30.0	34.0
4	35.78118461604593	37.0	35.0	37.0	35.0	37.0
5	35.346486847984835	37.0	35.0	37.0	33.0	37.0
6	35.32526008631057	37.0	35.0	37.0	32.0	37.0
7	35.32011384118016	37.0	35.0	37.0	32.0	37.0
8	35.26159579583362	37.0	35.0	37.0	32.0	37.0
9	36.93882586038161	39.0	37.0	39.0	33.0	39.0
10	36.715608977338725	38.0	37.0	39.0	32.0	39.0
11	36.881029568917	39.0	37.0	39.0	33.0	39.0
12	36.79182298836097	38.0	37.0	39.0	33.0	39.0
13	36.87260544154444	39.0	37.0	39.0	33.0	39.0
14	37.96813786498804	39.0	37.0	40.0	33.0	41.0
15	37.94026037121342	39.0	37.0	40.0	33.0	41.0
16	37.82616727695856	39.0	37.0	40.0	33.0	41.0
17	37.855551257021354	39.0	37.0	40.0	33.0	41.0
18	37.87031522250913	39.0	37.0	40.0	33.0	41.0
19	37.92802154465328	39.0	37.0	40.0	33.0	41.0
20	37.90444658371495	39.0	37.0	40.0	33.0	41.0
21	37.86218799389408	39.0	37.0	40.0	33.0	41.0
22	37.83399360817961	39.0	37.0	40.0	33.0	41.0
23	37.773018400725356	39.0	37.0	40.0	33.0	41.0
24	37.68969681338663	39.0	37.0	40.0	33.0	41.0
25	37.59885678983466	39.0	37.0	40.0	32.0	41.0
26	37.460019133475484	39.0	37.0	40.0	32.0	41.0
27	37.36130879370135	39.0	36.0	40.0	32.0	41.0
28	37.264324865220956	39.0	36.0	40.0	32.0	41.0
29	37.09505158740869	39.0	36.0	40.0	31.0	41.0
30	37.01876260195274	39.0	36.0	40.0	31.0	41.0
31	36.8784074630551	39.0	36.0	40.0	31.0	41.0
32	36.77308237890879	39.0	36.0	40.0	31.0	41.0
33	36.60406501383029	38.0	35.0	40.0	30.0	41.0
34	36.56781137632068	38.0	35.0	40.0	30.0	41.0
35	36.53346108976839	38.0	35.0	40.0	30.0	41.0
36	36.497008520094646	38.0	35.0	40.0	30.0	41.0
37	36.45344737444531	38.0	35.0	40.0	30.0	41.0
38	36.38799469380941	38.0	35.0	40.0	30.0	41.0
39	36.16825062653635	38.0	35.0	40.0	30.0	41.0
40	36.15785017309098	38.0	35.0	40.0	30.0	41.0
41	36.12089777385911	38.0	35.0	40.0	30.0	41.0
42	35.99657716718599	38.0	35.0	40.0	29.0	41.0
43	35.02019311414808	38.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	5.0
16	10.0
17	17.0
18	50.0
19	136.0
20	219.0
21	421.0
22	759.0
23	1265.0
24	1917.0
25	2933.0
26	4603.0
27	6472.0
28	9359.0
29	13203.0
30	17678.0
31	23241.0
32	31208.0
33	42067.0
34	59379.0
35	85558.0
36	129771.0
37	197567.0
38	254254.0
39	118242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.740884358433775	17.613093934968177	12.264717731253642	26.381303975344405
2	19.53343909726783	20.46702074592564	32.999647120331964	26.999893036474564
3	20.577083214623812	21.14079099027232	29.590909499860548	28.691216295243322
4	14.974393731737479	15.43483672067825	33.07122271305485	36.51954683452943
5	16.006041939698562	35.20939359678349	32.86629259422537	15.918271869292571
6	36.24094183883296	33.67501681926463	15.169127327581295	14.914914014321118
7	31.24294615536102	29.50393915674755	19.4301743105601	19.82294037733133
8	29.2726180372493	31.863734466546905	19.205750838963915	19.65789665723988
9	27.852702228540068	12.907198645261966	17.749947267981618	41.49015185821635
10	17.475340908750116	26.106897547936153	31.70758771259001	24.710173830723722
11	38.2494569351851	20.271187525053957	19.84463298015377	21.634722559607177
12	23.234277111504976	24.344798423737505	27.127849403353455	25.293075061404064
13	32.7790223533775	17.683369970839944	23.34713862572863	26.190469050053935
14	24.244032784820376	18.77379813483602	23.650435201596256	33.33173387874735
15	26.77047126929717	25.901467599548557	21.08640953434879	26.241651596805486
16	27.37226605727447	24.035603859084056	22.20232900580902	26.38980107783246
17	25.542589976817904	24.990978076475923	23.187393098953258	26.27903884775292
18	25.17101668331099	23.26476671455034	24.9295990067387	26.634617595399966
19	27.373065784567462	23.848367706612045	24.386684140708017	24.391882368112476
20	27.5335110727242	22.948574536083196	24.105480031309323	25.412434359883278
21	27.170634813528586	23.697019316413105	22.904289637233706	26.22805623282461
22	27.846104478372872	22.948074706525077	23.10022282401701	26.105597991085038
23	25.902867122311292	23.570962301855065	23.64933557656839	26.876834999265252
24	26.447581374751213	23.71971157835178	23.427011389116313	26.405695657780697
25	26.575237843895234	23.07613103931559	23.907447560381907	26.441183556407267
26	26.60732690152658	23.35083736445872	23.247072748192863	26.794762985821833
27	26.54814708184509	23.281461021791568	23.240275066202425	26.930116830160916
28	25.942053759667953	23.862462900151048	23.92054309480467	26.274940245376328
29	26.184371129444862	24.16605937375355	24.060895234724956	25.588674262076633
30	26.161279003859683	24.093184224179552	23.261867703113236	26.483669068847522
31	25.784607448859937	23.599052723021448	23.958230243486973	26.65810958463164
32	24.97158468962084	24.099182178877	24.185452760608634	26.74378037089353
33	25.558984386324262	23.15150533668019	24.626602328605944	26.6629079483896
34	26.330521292239347	23.068733561855407	24.627801919545437	25.972943226359813
35	26.08050654726738	23.03964348157278	24.436667096520086	26.44318287463975
36	25.015969554381957	23.071932471027377	24.5419312014603	27.37016677313036
37	25.12513232987551	22.018391728420607	25.829792040914047	27.026683900789834
38	24.502044802722274	21.98240400023592	26.076707842625662	27.438843354416143
39	24.33670118489595	22.041283922182537	27.22051780342903	26.40149708949248
40	24.699077614533444	21.22876099250156	27.587692596824482	26.484468796140515
41	23.20948556542219	21.55125102340102	28.57025754217812	26.669005868998674
42	22.926382103702636	21.5130640451606	28.61474237285086	26.945811478285904
43	22.289699212568514	20.648558841435072	28.978218427516218	28.083523518480195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	15.5
2	23.0
3	78.5
4	134.0
5	134.0
6	205.0
7	276.0
8	315.5
9	355.0
10	526.0
11	697.0
12	697.0
13	1163.0
14	1629.0
15	3035.0
16	4441.0
17	4202.5
18	3964.0
19	3964.0
20	4234.0
21	4504.0
22	4333.5
23	4163.0
24	4883.0
25	5603.0
26	5603.0
27	6407.5
28	7212.0
29	9526.5
30	11841.0
31	13941.5
32	16042.0
33	16042.0
34	20059.5
35	24077.0
36	27079.5
37	30082.0
38	34485.0
39	38888.0
40	38888.0
41	43629.0
42	48370.0
43	51781.0
44	55192.0
45	59488.0
46	63784.0
47	63784.0
48	67452.5
49	71121.0
50	76072.5
51	81024.0
52	87057.0
53	93090.0
54	93090.0
55	87927.0
56	82764.0
57	76889.0
58	71014.0
59	68778.5
60	66543.0
61	66543.0
62	62364.5
63	58186.0
64	50269.5
65	42353.0
66	38101.0
67	33849.0
68	33849.0
69	29949.0
70	26049.0
71	24628.5
72	23208.0
73	19483.5
74	15759.0
75	15759.0
76	10800.0
77	5841.0
78	4775.0
79	3709.0
80	2938.5
81	2168.0
82	2168.0
83	1661.5
84	1155.0
85	926.0
86	697.0
87	507.5
88	318.0
89	318.0
90	239.0
91	160.0
92	96.5
93	33.0
94	21.5
95	10.0
96	10.0
97	6.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1000341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.58347378777164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.93715722354945	53.275384596240606
2	6.643422607232879	8.049632387727652
3	1.9423974877654588	3.530315618564165
4	0.9041057205781895	2.1909546089609253
5	0.5446162935627491	1.6497373472726076
6	0.3417850702542614	1.242391610688044
7	0.242564416030841	1.0286776458315412
8	0.1805201057352628	0.8749228075182448
9	0.13398084527038828	0.730532252475185
>10	0.9327016199438974	11.154762323345134
>50	0.11340710884160762	4.753961923590316
>100	0.07786287686431546	8.935529991030066
>500	0.004814548689330592	1.9053880708131978
>1k	6.640756812869782E-4	0.6778088159422583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2297	0.22962169900064078	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1679	0.16784276561692463	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1631	0.16304440185896607	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1132	0.11316141195852214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.996591162413617E-5	0.0	0.0	0.0	0.0
6	9.996591162413617E-5	0.0	0.0	0.0	0.0
7	9.996591162413617E-5	0.0	0.0	0.0	0.0
8	9.996591162413617E-5	0.0	0.0	0.0	0.0
9	9.996591162413617E-5	0.0	0.0	2.998977348724085E-4	0.0
10	9.996591162413617E-5	0.0	0.0	4.998295581206809E-4	0.0
11	9.996591162413617E-5	0.0	0.0	4.998295581206809E-4	0.0
12	9.996591162413617E-5	0.0	0.0	6.997613813689532E-4	0.0
13	9.996591162413617E-5	0.0	0.0	7.997272929930894E-4	0.0
14	9.996591162413617E-5	0.0	0.0	7.997272929930894E-4	0.0
15	9.996591162413617E-5	0.0	0.0	9.996591162413618E-4	0.0
16	9.996591162413617E-5	0.0	0.0	0.0015994545859861788	0.0
17	9.996591162413617E-5	0.0	0.0	0.0020992841441068596	0.0
18	9.996591162413617E-5	0.0	0.0	0.002499147790603404	0.0
19	9.996591162413617E-5	0.0	0.0	0.003098943260348221	0.0
20	9.996591162413617E-5	0.0	0.0	0.003698738730093038	0.0
21	9.996591162413617E-5	0.0	0.0	0.0046983978463344	0.0
22	9.996591162413617E-5	0.0	0.0	0.006397818343944715	0.0
23	9.996591162413617E-5	0.0	0.0	0.009996591162413616	0.0
24	9.996591162413617E-5	0.0	0.0	0.01459502309712388	0.0
25	9.996591162413617E-5	0.0	0.0	0.016794273152854877	0.0
26	9.996591162413617E-5	0.0	0.0	0.020493011882947915	0.0
27	9.996591162413617E-5	0.0	0.0	0.042485512440257875	0.0
28	9.996591162413617E-5	0.0	0.0	0.13175507152061147	0.0
29	9.996591162413617E-5	0.0	0.0	0.2890014505053777	0.0
30	9.996591162413617E-5	0.0	0.0	0.4837350463491949	0.0
31	9.996591162413617E-5	0.0	0.0	1.0077563550829167	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	865	0.0	30.369942	1
TATAACT	65	2.682269E-6	22.76923	4
TACTATA	70	5.10083E-6	21.142859	2
CATAATA	45	0.003825466	20.555555	2
CTATACG	50	0.00703426	18.5	4
TAACGTT	50	0.00703426	18.5	18
ATATAAC	60	9.2356704E-4	18.5	3
ATAACGT	50	0.00703426	18.5	17
GTATCAA	1520	0.0	17.282894	2
ATACACT	75	2.0669289E-4	17.266666	4
ATTTAGG	65	0.0015797404	17.076923	2
GTAAATC	65	0.0015797404	17.076923	16
AATCGAG	65	0.0015797404	17.076923	22
GTACTAT	120	1.0407712E-7	16.958334	1
CTTATAC	1115	0.0	16.923767	37
GTGTTAG	80	3.3820161E-4	16.1875	1
TCTAGAC	80	3.3820161E-4	16.1875	3
TCTACAC	70	0.0025921778	15.857143	3
CCATACA	70	0.0025921778	15.857143	4
ACATATA	70	0.0025921778	15.857143	1
>>END_MODULE