FastQCFastQC Report
Fri 10 Feb 2017
ERR1632070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620709
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.22893175384922723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9690.15611180118219647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8760.14112893481486494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.024.6666661
ATGGTCG400.00193019223.12536
TATAGAG400.00193019223.1255
GTAGTTC1005.329639E-1022.1999993
TAGTTCC855.171023E-821.7647064
GGCTAAG806.9495036E-720.81251
CTAGGAC851.2434211E-619.5882363
TATATAC801.6147189E-518.53
CTATTAG609.2302903E-418.51
ATAAACG801.6147189E-518.515
TATACTG609.2302903E-418.55
TAATGGT609.2302903E-418.54
AATAACG609.2302903E-418.52
TACCCCG500.00703132718.4999985
TAACGCC500.00703132718.4999984
TAACGAA500.00703132718.49999817
TCAATAC500.00703132718.4999983
ATAACGC500.00703132718.4999983
TAAACGA953.6009824E-617.52631616
CGGCGAT852.7198495E-517.41176430
Produced by FastQC (version 0.11.5)