##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620709
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17210641379455	31.0	31.0	34.0	28.0	34.0
2	31.339395755498952	31.0	31.0	34.0	30.0	34.0
3	31.361111245366185	31.0	31.0	34.0	28.0	34.0
4	35.25801462521085	37.0	35.0	37.0	33.0	37.0
5	34.27262855863214	35.0	35.0	37.0	30.0	37.0
6	34.22447717046152	35.0	35.0	37.0	30.0	37.0
7	34.24525018970242	35.0	35.0	37.0	30.0	37.0
8	34.16376595151673	35.0	35.0	37.0	30.0	37.0
9	35.713774087374276	37.0	35.0	39.0	30.0	39.0
10	35.35072151362394	37.0	34.0	39.0	30.0	39.0
11	35.60266082818197	37.0	35.0	39.0	30.0	39.0
12	35.497737264966354	37.0	35.0	39.0	30.0	39.0
13	35.62588427105133	37.0	35.0	39.0	30.0	39.0
14	36.52226566716448	38.0	36.0	40.0	31.0	41.0
15	36.46091163492071	38.0	35.0	40.0	31.0	41.0
16	36.334664069636496	38.0	35.0	40.0	30.0	41.0
17	36.35922630411352	38.0	35.0	40.0	30.0	41.0
18	36.3728188249244	38.0	35.0	40.0	30.0	41.0
19	36.44789587391193	38.0	35.0	40.0	30.0	41.0
20	36.40695559432842	38.0	35.0	40.0	30.0	41.0
21	36.36314762634343	38.0	35.0	40.0	30.0	41.0
22	36.330077379255016	38.0	35.0	40.0	30.0	41.0
23	36.241750965428245	38.0	35.0	40.0	30.0	41.0
24	36.123341211421135	38.0	34.0	40.0	30.0	41.0
25	36.00690500701617	38.0	34.0	40.0	29.0	41.0
26	35.854778970499865	38.0	34.0	40.0	29.0	41.0
27	35.7198155657482	38.0	34.0	40.0	29.0	41.0
28	35.59607803334574	38.0	34.0	40.0	27.0	41.0
29	35.39824781016547	38.0	34.0	40.0	27.0	41.0
30	35.29835881226146	38.0	34.0	40.0	27.0	41.0
31	35.156856111317865	38.0	34.0	40.0	27.0	41.0
32	35.02723176238785	38.0	33.0	40.0	27.0	40.0
33	34.842496242200454	37.0	33.0	40.0	26.0	40.0
34	34.81308954759799	37.0	33.0	40.0	26.0	40.0
35	34.75380411754945	37.0	33.0	39.0	26.0	40.0
36	34.71311194134449	37.0	33.0	39.0	26.0	40.0
37	34.69057481041841	37.0	33.0	39.0	26.0	40.0
38	34.58460405761798	37.0	33.0	39.0	25.0	40.0
39	34.386599839860544	37.0	33.0	39.0	25.0	40.0
40	34.349168450916615	37.0	33.0	39.0	25.0	40.0
41	34.33885443903665	37.0	33.0	39.0	25.0	40.0
42	34.191095988619466	37.0	32.0	39.0	24.0	40.0
43	33.23935531787037	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	3.0
13	7.0
14	11.0
15	19.0
16	44.0
17	77.0
18	160.0
19	288.0
20	503.0
21	779.0
22	1236.0
23	1960.0
24	2924.0
25	4364.0
26	6214.0
27	8646.0
28	12221.0
29	16124.0
30	21573.0
31	27726.0
32	36216.0
33	46520.0
34	59345.0
35	73523.0
36	89482.0
37	97349.0
38	83087.0
39	30304.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.609675387339315	19.44083298292759	12.970329091409985	25.97916253832311
2	19.399750929984904	21.675213344739642	33.22748663222219	25.69754909305327
3	20.37653715348094	22.3542755139687	30.477405676411973	26.791781656138387
4	15.743931536356007	16.142508002945018	34.133869494400756	33.979690966298215
5	15.47069560776467	35.577541166633644	33.74689266628968	15.204870559312011
6	34.61009909635594	35.23229081582513	15.285584710387637	14.872025377431292
7	29.529296336930834	30.619984566036578	20.63543464006483	19.21528445696776
8	28.171010892382743	32.64766581441545	19.864219787372186	19.317103505829625
9	27.312959857195562	13.905872155873364	18.79415313778276	39.98701484914831
10	17.083528674467424	26.89988384250913	32.397629162779985	23.618958320243465
11	36.770370656781196	21.179489905897935	20.90109858242751	21.149040854893357
12	22.625578169480384	25.138672066942803	28.313589782007348	23.922159981569465
13	31.838107712309633	18.945270650175846	23.647635204258354	25.568986433256164
14	23.58754263269906	20.26940160365002	24.36052965238139	31.782526111269533
15	26.27769212303994	26.84204675620943	21.520712604457163	25.35954851629346
16	25.622634761216606	25.778424350218863	22.989033508455652	25.609907380108876
17	24.39258976428568	25.861555092644057	24.643109734191064	25.1027454088792
18	24.47427055190113	24.3643961985407	25.881693353890473	25.279639895667692
19	25.370503730411514	24.77457230360765	25.659044737550126	24.195879228430712
20	26.162662374800426	24.111298531195775	25.133355565973748	24.592683528030044
21	26.3318237692703	24.73381246284491	24.09277133084908	24.84159243703571
22	26.391594128649658	24.733490250664968	24.14351974919004	24.731395871495337
23	25.836583648698504	24.555790233426613	24.306559112241004	25.301067005633882
24	25.60604083394956	24.62297147294465	24.54193510968908	25.229052583416706
25	25.63069006571517	24.075371873132177	24.674525421735467	25.619412639417185
26	25.18410398431471	24.49005894871832	24.681291877514262	25.644545189452707
27	25.383070005429275	24.427871997989396	24.56384553792518	25.62521245865615
28	25.019453560364035	24.61298289536643	25.153816039400105	25.21374750486943
29	24.763133771219685	24.86479171399158	25.26433481712042	25.107739697668315
30	24.90055726596521	25.351815423974845	24.874458079389857	24.87316923067009
31	25.26675140846999	25.010753831505582	24.626032488654104	25.096462271370317
32	24.443821500896558	24.949372411226516	25.157521479469448	25.44928460840748
33	24.56384553792518	24.68596395412343	25.494716525779392	25.255473982172
34	25.099845499259715	24.113715122545347	25.43655722729975	25.349882150895187
35	24.734617993294762	24.383084504977372	25.892487461918545	24.989810039809317
36	24.457676624634086	24.532268744290803	25.548042641559892	25.462011989515215
37	24.7995437475532	23.483790310757538	26.07389291922624	25.64277302246302
38	24.168813405315532	23.619924956783294	26.70252888229428	25.508732755606893
39	24.047017201297226	23.29674614030085	27.03263526064549	25.623601397756435
40	23.487495750826877	22.90606387212043	28.172138635012544	25.434301742040148
41	23.025282378699195	22.739963493360012	28.72795464541355	25.50679948252724
42	23.04429289731581	22.524564651068374	28.767908955726433	25.663233495889376
43	22.15450396240428	22.0033864500112	29.327108194016844	26.515001393567676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	23.5
2	23.0
3	67.0
4	111.0
5	111.0
6	156.0
7	201.0
8	217.5
9	234.0
10	354.5
11	475.0
12	475.0
13	821.5
14	1168.0
15	2052.5
16	2937.0
17	2832.5
18	2728.0
19	2728.0
20	3184.0
21	3640.0
22	4006.0
23	4372.0
24	5297.0
25	6222.0
26	6222.0
27	7294.0
28	8366.0
29	10313.0
30	12260.0
31	14233.0
32	16206.0
33	16206.0
34	18235.0
35	20264.0
36	22916.5
37	25569.0
38	27614.0
39	29659.0
40	29659.0
41	32113.0
42	34567.0
43	35497.0
44	36427.0
45	38083.0
46	39739.0
47	39739.0
48	41014.0
49	42289.0
50	44028.0
51	45767.0
52	47885.5
53	50004.0
54	50004.0
55	47853.5
56	45703.0
57	44352.0
58	43001.0
59	39949.0
60	36897.0
61	36897.0
62	34150.5
63	31404.0
64	28022.5
65	24641.0
66	21427.0
67	18213.0
68	18213.0
69	15542.0
70	12871.0
71	11210.5
72	9550.0
73	7787.5
74	6025.0
75	6025.0
76	4857.5
77	3690.0
78	3023.5
79	2357.0
80	1867.5
81	1378.0
82	1378.0
83	1118.5
84	859.0
85	658.5
86	458.0
87	352.5
88	247.0
89	247.0
90	179.5
91	112.0
92	76.5
93	41.0
94	24.5
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	620709.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.4784965191153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.02106767769752	70.37632904074063
2	4.816641548816316	7.367390078499494
3	1.1810633644723254	2.7097785122595406
4	0.5414512746310788	1.6563751768857402
5	0.29252651539794605	1.1185994044805374
6	0.2008318329646289	0.921558998298775
7	0.14206336343235199	0.7605354712028459
8	0.09936489326652315	0.6079422115044844
9	0.07679573593368483	0.5285900180958492
>10	0.5512803976676459	8.349243345531413
>50	0.05699649767966401	2.9702792853430195
>100	0.019281252358282184	2.104142469176674
>500	4.2376378806100373E-4	0.2989713404241392
>1k	2.1188189403050187E-4	0.23026464755701992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1421	0.22893175384922723	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	969	0.15611180118219647	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	876	0.14112893481486494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6110608997130701E-4	0.0	1.6110608997130701E-4	0.0
3	0.0	3.2221217994261403E-4	0.0	1.6110608997130701E-4	0.0
4	0.0	3.2221217994261403E-4	0.0	3.2221217994261403E-4	0.0
5	0.0	3.2221217994261403E-4	0.0	3.2221217994261403E-4	0.0
6	0.0	3.2221217994261403E-4	0.0	3.2221217994261403E-4	0.0
7	0.0	3.2221217994261403E-4	0.0	3.2221217994261403E-4	0.0
8	0.0	3.2221217994261403E-4	0.0	3.2221217994261403E-4	0.0
9	0.0	3.2221217994261403E-4	0.0	6.444243598852281E-4	0.0
10	0.0	3.2221217994261403E-4	0.0	8.05530449856535E-4	0.0
11	0.0	3.2221217994261403E-4	0.0	9.66636539827842E-4	0.0
12	0.0	3.2221217994261403E-4	0.0	0.001127742629799149	0.0
13	0.0	3.2221217994261403E-4	0.0	0.001449954809741763	0.0
14	0.0	3.2221217994261403E-4	0.0	0.001449954809741763	0.0
15	0.0	3.2221217994261403E-4	0.0	0.001449954809741763	0.0
16	0.0	3.2221217994261403E-4	0.0	0.001933273079655684	0.0
17	0.0	3.2221217994261403E-4	0.0	0.002416591349569605	0.0
18	0.0	3.2221217994261403E-4	0.0	0.002416591349569605	0.0
19	0.0	3.2221217994261403E-4	0.0	0.0025776974395409122	0.0
20	0.0	3.2221217994261403E-4	0.0	0.002738803529512219	0.0
21	0.0	3.2221217994261403E-4	0.0	0.003866546159311368	0.0
22	0.0	3.2221217994261403E-4	0.0	0.0046720766091679035	0.0
23	0.0	4.83318269913921E-4	0.0	0.005960925328938359	0.0
24	0.0	4.83318269913921E-4	0.0	0.008699728858450578	0.0
25	0.0	4.83318269913921E-4	0.0	0.010149683668192341	0.0
26	0.0	4.83318269913921E-4	0.0	0.013210699377647175	0.0
27	0.0	4.83318269913921E-4	0.0	0.028676884014892646	0.0
28	0.0	4.83318269913921E-4	0.0	0.08216410588536657	0.0
29	0.0	6.444243598852281E-4	0.0	0.165294848310561	0.0
30	0.0	6.444243598852281E-4	0.0	0.2790357478303037	0.0
31	0.0	6.444243598852281E-4	0.0	0.5857817431356722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	450	0.0	24.666666	1
ATGGTCG	40	0.001930192	23.125	36
TATAGAG	40	0.001930192	23.125	5
GTAGTTC	100	5.329639E-10	22.199999	3
TAGTTCC	85	5.171023E-8	21.764706	4
GGCTAAG	80	6.9495036E-7	20.8125	1
CTAGGAC	85	1.2434211E-6	19.588236	3
TATATAC	80	1.6147189E-5	18.5	3
CTATTAG	60	9.2302903E-4	18.5	1
ATAAACG	80	1.6147189E-5	18.5	15
TATACTG	60	9.2302903E-4	18.5	5
TAATGGT	60	9.2302903E-4	18.5	4
AATAACG	60	9.2302903E-4	18.5	2
TACCCCG	50	0.007031327	18.499998	5
TAACGCC	50	0.007031327	18.499998	4
TAACGAA	50	0.007031327	18.499998	17
TCAATAC	50	0.007031327	18.499998	3
ATAACGC	50	0.007031327	18.499998	3
TAAACGA	95	3.6009824E-6	17.526316	16
CGGCGAT	85	2.7198495E-5	17.411764	30
>>END_MODULE