FastQCFastQC Report
Fri 10 Feb 2017
ERR1632069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271474
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4490.1653933710042214No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4370.16097305819341817No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG3720.13702969713490057No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG3240.1193484458916876No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3100.11419141427908382No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT3070.113086336076383No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3060.11271797667548275No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC3040.11198125787368218No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC2780.10240391345027516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAT250.00548904229.619
GTGTATA250.00548904229.69
GGTATCA1100.025.2272721
GAACAGC652.670331E-622.7692286
AGTTTGA450.003818397220.5555558
TTTAACT450.003818397220.5555552
GACGCAG450.003818397220.55555524
AGATAGA500.00702138518.51
ATATCAC902.139921E-618.53
GATAGAA500.00702138518.52
CTTATAC1950.017.07692337
AGGACAC1655.2750693E-1116.8181825
TTAGGAC1302.56754E-715.6538453
ACATCTA957.0279726E-515.57894824
CGGACAT855.3454284E-415.23529421
TATCACT855.3454284E-415.2352944
CTTAGGA1101.4436824E-515.1363642
TACACCT1101.4436824E-515.1363644
CATCTAA1001.0884851E-414.825
GTATCAA2006.184564E-1114.81
Produced by FastQC (version 0.11.5)