##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	271474
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42740372927057	33.0	31.0	34.0	31.0	34.0
2	32.600856803966494	34.0	31.0	34.0	31.0	34.0
3	32.60399522606217	34.0	31.0	34.0	31.0	34.0
4	36.16655001952305	37.0	35.0	37.0	35.0	37.0
5	36.007411391146114	37.0	35.0	37.0	35.0	37.0
6	36.02945770128999	37.0	35.0	37.0	35.0	37.0
7	36.019961395934786	37.0	35.0	37.0	35.0	37.0
8	35.997800894376624	37.0	35.0	37.0	35.0	37.0
9	37.724220367327995	39.0	37.0	39.0	35.0	39.0
10	37.61709408635818	39.0	37.0	39.0	35.0	39.0
11	37.73377929378135	39.0	37.0	39.0	35.0	39.0
12	37.69783478344151	39.0	37.0	39.0	35.0	39.0
13	37.713685288462244	39.0	37.0	39.0	35.0	39.0
14	39.042129264680966	40.0	38.0	41.0	36.0	41.0
15	39.050063726176354	40.0	38.0	41.0	36.0	41.0
16	38.92836514730693	40.0	38.0	41.0	36.0	41.0
17	38.972509337910815	40.0	38.0	41.0	36.0	41.0
18	38.978259428158864	40.0	38.0	41.0	36.0	41.0
19	39.03093850608161	40.0	39.0	41.0	36.0	41.0
20	39.006954625488994	40.0	38.0	41.0	36.0	41.0
21	38.9725461738509	40.0	38.0	41.0	36.0	41.0
22	38.95974200107561	40.0	38.0	41.0	36.0	41.0
23	38.92409217825648	40.0	38.0	41.0	36.0	41.0
24	38.864023810751675	40.0	38.0	41.0	35.0	41.0
25	38.78263848471677	40.0	38.0	41.0	35.0	41.0
26	38.69495421292647	40.0	38.0	41.0	35.0	41.0
27	38.61605899644165	40.0	38.0	41.0	35.0	41.0
28	38.550369464479104	40.0	38.0	41.0	35.0	41.0
29	38.42185623669302	40.0	38.0	41.0	34.0	41.0
30	38.36848095950257	40.0	38.0	41.0	34.0	41.0
31	38.29769333343156	40.0	38.0	41.0	34.0	41.0
32	38.22604006276844	40.0	38.0	41.0	34.0	41.0
33	38.09740527638006	40.0	38.0	41.0	34.0	41.0
34	38.09590237002438	40.0	38.0	41.0	33.0	41.0
35	38.077281802308875	40.0	38.0	41.0	33.0	41.0
36	38.0511872223491	40.0	38.0	41.0	33.0	41.0
37	38.03018705290378	40.0	38.0	41.0	33.0	41.0
38	37.95943626277286	40.0	37.0	41.0	33.0	41.0
39	37.7722581167994	40.0	37.0	41.0	33.0	41.0
40	37.796577204446834	40.0	37.0	41.0	33.0	41.0
41	37.800374989870114	40.0	37.0	41.0	33.0	41.0
42	37.69256356041463	40.0	37.0	41.0	33.0	41.0
43	36.83512601575105	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	4.0
18	3.0
19	15.0
20	30.0
21	53.0
22	103.0
23	161.0
24	259.0
25	407.0
26	625.0
27	848.0
28	1268.0
29	1856.0
30	2479.0
31	3317.0
32	4446.0
33	6258.0
34	8652.0
35	12741.0
36	20094.0
37	36446.0
38	81048.0
39	90358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.509411582693005	17.86985125647392	12.677825500784607	26.942911660048473
2	20.12236899297907	20.348173305730935	33.22822811760979	26.301229583680204
3	20.99022374150011	21.263178057567206	29.444071992161312	28.302526208771372
4	16.35957771278281	15.2607616198973	31.965860450724566	36.41380021659533
5	16.49734412871951	34.597788370157	32.918069502051765	15.986797999071733
6	36.71806508173895	32.39757766857968	15.048586604978745	15.835770644702624
7	30.53036386541621	28.564061383410568	20.21777407781224	20.687800673360986
8	27.530813263885307	32.38394837074637	19.573145126236767	20.512093239131556
9	26.791884305679364	14.10079786646235	18.619830996706867	40.48748683115142
10	17.989936421167403	26.167515121153407	31.983541701967777	23.859006755711412
11	37.355326845296425	20.495148706690145	20.27523814435268	21.874286303660757
12	22.854859028857273	24.217420452787376	27.078836278980678	25.848884239374676
13	31.577241282774775	18.499745832013378	23.478491494581434	26.44452139063041
14	24.036924346346243	19.844257645299365	22.66773245319994	33.45108555515445
15	27.1079366716518	26.48430420592764	20.479309252451433	25.928449869969132
16	27.06741713755277	24.868311514178153	22.023471861025364	26.040799487243717
17	25.85514634918998	25.036651760389578	23.304625857356505	25.80357603306394
18	24.913251361087987	23.85421808349971	24.843263074916937	26.38926748049537
19	26.538821397260882	24.07781223984617	24.29035561416563	25.093010748727316
20	26.736262036143426	23.33777820343753	24.56552008663813	25.360439673780917
21	26.44304795302681	24.273042722323314	23.135180532942382	26.148728791707494
22	26.588181556981517	23.51311727826606	23.262632885653876	26.636068279098552
23	25.781106109609027	24.05865755099936	23.533008685914673	26.627227653476943
24	27.055261277323055	24.154799354634328	23.222481710955744	25.567457657086866
25	26.663695234166074	23.47517625997333	23.67482705526128	26.18630145059932
26	26.697215939647993	23.75954971746834	23.24274147800526	26.300492864878404
27	26.317437397319814	23.327464140212324	23.51716923167596	26.8379292307919
28	25.452897883406884	23.816277065206982	24.136013025188415	26.594812026197722
29	25.689752978185755	24.052395441184053	24.013717704089526	26.244133876540666
30	25.252878728718038	25.013076758731962	23.74923565424313	25.984808858306874
31	26.4301553739953	23.819223940414183	23.90431496202215	25.84630572356837
32	25.16410411310107	23.867110662531218	24.32056108503945	26.648224139328256
33	25.319920139681884	23.37903445633836	24.329770070061958	26.9712753339178
34	25.987019014712274	23.31015124837001	24.26825405011161	26.434575686806106
35	25.967127607063663	23.319360233392516	24.36918452595829	26.344327633585536
36	25.280505683785552	23.488805557806643	24.920986908506894	26.30970184990091
37	26.10452566359946	22.09677538180452	25.034809963385072	26.763888991210944
38	24.795007993398997	22.53364963127224	26.441206156022307	26.230136219306456
39	24.682658376124415	22.048520300286583	26.902023766548545	26.366797557040456
40	23.997141531049014	21.491929245526276	27.98573712399714	26.52519209942757
41	23.33556804703213	21.953851934255216	28.47381333019	26.236766688522657
42	23.899157930409544	21.34679564157157	28.10840080449693	26.645645623521958
43	22.49202501897051	21.628958942661175	28.76039694409041	27.118619094277907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	6.0
2	1.0
3	4.0
4	7.0
5	7.0
6	16.5
7	26.0
8	32.0
9	38.0
10	49.0
11	60.0
12	60.0
13	109.0
14	158.0
15	259.0
16	360.0
17	374.0
18	388.0
19	388.0
20	502.5
21	617.0
22	775.5
23	934.0
24	1192.5
25	1451.0
26	1451.0
27	1667.5
28	1884.0
29	2889.5
30	3895.0
31	4394.0
32	4893.0
33	4893.0
34	5732.5
35	6572.0
36	7479.0
37	8386.0
38	10260.5
39	12135.0
40	12135.0
41	13470.5
42	14806.0
43	14809.0
44	14812.0
45	16409.0
46	18006.0
47	18006.0
48	19525.5
49	21045.0
50	22610.5
51	24176.0
52	25743.5
53	27311.0
54	27311.0
55	24456.5
56	21602.0
57	20217.5
58	18833.0
59	17458.5
60	16084.0
61	16084.0
62	15063.5
63	14043.0
64	12794.5
65	11546.0
66	10090.5
67	8635.0
68	8635.0
69	7464.5
70	6294.0
71	5505.5
72	4717.0
73	3902.0
74	3087.0
75	3087.0
76	2473.5
77	1860.0
78	1546.0
79	1232.0
80	985.5
81	739.0
82	739.0
83	573.5
84	408.0
85	312.5
86	217.0
87	171.0
88	125.0
89	125.0
90	96.0
91	67.0
92	38.5
93	10.0
94	6.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	271474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.20566382431021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.20503492886381	59.720842177605185
2	5.23497715120835	6.931702748016903
3	1.4790761303670035	2.937696511729184
4	0.7681088529317086	2.0341262599069294
5	0.43691506282924586	1.4463125884725212
6	0.27759560570839237	1.1027040810381357
7	0.2101856853300492	0.9740837976550442
8	0.17204590625751792	0.9112330745627218
9	0.13096121302699346	0.7803336639320103
>10	0.9207554341296066	12.234939056140908
>50	0.11235765271705174	5.128270936195928
>100	0.051986376630277664	5.797755104744564
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	449	0.1653933710042214	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	437	0.16097305819341817	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	372	0.13702969713490057	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	324	0.1193484458916876	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	310	0.11419141427908382	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	307	0.113086336076383	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	306	0.11271797667548275	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	304	0.11198125787368218	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	278	0.10240391345027516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	3.6835940090027035E-4	0.0	0.0	0.0
12	0.0	3.6835940090027035E-4	0.0	0.0	0.0
13	0.0	3.6835940090027035E-4	0.0	0.0	0.0
14	0.0	3.6835940090027035E-4	0.0	0.0	0.0
15	0.0	3.6835940090027035E-4	0.0	0.0	0.0
16	0.0	3.6835940090027035E-4	0.0	0.0	0.0
17	0.0	3.6835940090027035E-4	0.0	0.0	0.0
18	0.0	3.6835940090027035E-4	0.0	0.0	0.0
19	0.0	3.6835940090027035E-4	0.0	0.0	0.0
20	0.0	3.6835940090027035E-4	0.0	3.6835940090027035E-4	0.0
21	0.0	3.6835940090027035E-4	0.0	0.0014734376036010814	0.0
22	0.0	3.6835940090027035E-4	0.0	0.0018417970045013518	0.0
23	0.0	3.6835940090027035E-4	0.0	0.002946875207202163	0.0
24	0.0	3.6835940090027035E-4	0.0	0.005525391013504056	0.0
25	0.0	3.6835940090027035E-4	0.0	0.0062621098153045965	0.0
26	0.0	3.6835940090027035E-4	0.0	0.008472266220706218	0.0
27	0.0	3.6835940090027035E-4	0.0	0.030942189675622712	0.0
28	0.0	3.6835940090027035E-4	0.0	0.10093047584667408	0.0
29	0.0	7.367188018005407E-4	0.0	0.18307462224743437	0.0
30	0.0	7.367188018005407E-4	0.0	0.2980027553283187	0.0
31	0.0	7.367188018005407E-4	0.0	0.5901117602422331	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCAT	25	0.005489042	29.6	19
GTGTATA	25	0.005489042	29.6	9
GGTATCA	110	0.0	25.227272	1
GAACAGC	65	2.670331E-6	22.769228	6
AGTTTGA	45	0.0038183972	20.555555	8
TTTAACT	45	0.0038183972	20.555555	2
GACGCAG	45	0.0038183972	20.555555	24
AGATAGA	50	0.007021385	18.5	1
ATATCAC	90	2.139921E-6	18.5	3
GATAGAA	50	0.007021385	18.5	2
CTTATAC	195	0.0	17.076923	37
AGGACAC	165	5.2750693E-11	16.818182	5
TTAGGAC	130	2.56754E-7	15.653845	3
ACATCTA	95	7.0279726E-5	15.578948	24
CGGACAT	85	5.3454284E-4	15.235294	21
TATCACT	85	5.3454284E-4	15.235294	4
CTTAGGA	110	1.4436824E-5	15.136364	2
TACACCT	110	1.4436824E-5	15.136364	4
CATCTAA	100	1.0884851E-4	14.8	25
GTATCAA	200	6.184564E-11	14.8	1
>>END_MODULE