##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632068.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40733
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.442933248226254	31.0	31.0	34.0	30.0	34.0
2	31.638843198389512	31.0	31.0	34.0	30.0	34.0
3	31.649768001374806	31.0	31.0	34.0	30.0	34.0
4	35.474848403014754	37.0	35.0	37.0	33.0	37.0
5	34.791323987921345	37.0	35.0	37.0	32.0	37.0
6	34.748925932290774	35.0	35.0	37.0	32.0	37.0
7	34.75778852527435	35.0	35.0	37.0	32.0	37.0
8	34.67682223258783	35.0	35.0	37.0	31.0	37.0
9	36.298922249772914	38.0	35.0	39.0	32.0	39.0
10	36.014386369773895	37.0	35.0	39.0	31.0	39.0
11	36.225026391378	37.0	35.0	39.0	32.0	39.0
12	36.168732968354895	37.0	35.0	39.0	32.0	39.0
13	36.224977291139865	37.0	35.0	39.0	32.0	39.0
14	37.25583679080844	39.0	36.0	40.0	32.0	41.0
15	37.17452679645496	39.0	36.0	40.0	32.0	41.0
16	37.054820415879014	38.0	36.0	40.0	32.0	41.0
17	37.07156359708345	38.0	36.0	40.0	32.0	41.0
18	37.064591363268114	38.0	36.0	40.0	32.0	41.0
19	37.11911717771831	39.0	36.0	40.0	32.0	41.0
20	37.08315125328358	39.0	36.0	40.0	31.0	41.0
21	37.01527017406035	39.0	36.0	40.0	31.0	41.0
22	37.00616207988609	39.0	36.0	40.0	31.0	41.0
23	36.96813394544964	39.0	36.0	40.0	31.0	41.0
24	36.877617656445636	38.0	36.0	40.0	31.0	41.0
25	36.753369503842094	38.0	36.0	40.0	31.0	41.0
26	36.545233594382935	38.0	35.0	40.0	30.0	41.0
27	36.41924238332556	38.0	35.0	40.0	30.0	41.0
28	36.31581273169175	38.0	35.0	40.0	30.0	41.0
29	36.16082783001497	38.0	35.0	40.0	30.0	41.0
30	36.082291999116194	38.0	35.0	40.0	29.0	41.0
31	35.95107161269732	38.0	35.0	40.0	29.0	41.0
32	35.8468809073724	38.0	34.0	40.0	29.0	41.0
33	35.671961309012346	38.0	34.0	40.0	28.0	41.0
34	35.63133086195468	38.0	34.0	40.0	28.0	41.0
35	35.59546313799622	38.0	34.0	40.0	28.0	41.0
36	35.554390788795324	38.0	34.0	40.0	27.0	41.0
37	35.50605160435028	38.0	34.0	40.0	27.0	41.0
38	35.4431296491788	38.0	34.0	40.0	28.0	41.0
39	35.255443988903345	38.0	34.0	40.0	27.0	41.0
40	35.22929811209584	38.0	33.0	40.0	27.0	41.0
41	35.215721896251196	38.0	33.0	40.0	27.0	41.0
42	35.0557533204036	38.0	33.0	40.0	27.0	41.0
43	34.0730611543466	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	1.0
16	1.0
17	1.0
18	12.0
19	13.0
20	23.0
21	49.0
22	63.0
23	96.0
24	133.0
25	203.0
26	313.0
27	400.0
28	580.0
29	735.0
30	1022.0
31	1416.0
32	1833.0
33	2385.0
34	3295.0
35	4341.0
36	5771.0
37	7447.0
38	7628.0
39	2970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21638474946604	18.365944074828764	12.365894974590628	27.051776201114574
2	21.854515994402572	20.23175312400265	30.93315002577762	26.98058085581715
3	21.950261458768075	21.523089386983525	28.0730611543466	28.453587999901796
4	17.197358407188275	15.54759040581347	31.1467360616699	36.10831512532835
5	17.298013895367394	34.17622075467066	31.807134264601185	16.718631085360762
6	37.56168217415855	32.64674833672943	14.015662975965434	15.77590651314659
7	31.610733312056567	27.658164142096087	19.74320575454791	20.987896791299438
8	29.224461738639434	31.267031645103476	18.660545503645693	20.847961112611397
9	27.510863427687624	13.335624677779686	17.9019468244421	41.25156507009059
10	18.550069967839345	25.14177693761815	31.686838681167607	24.621314413374908
11	39.37593597328947	19.897871504676797	18.53288488449169	22.19330763754204
12	23.51655905531142	23.52392409103184	26.63196916505045	26.32754768860629
13	32.93398472982594	18.024697419782488	22.350428399577737	26.690889450813838
14	24.871725627869296	18.930596813394544	22.210492720889697	33.98718483784646
15	28.593523678589843	25.19578719956792	19.257113396999976	26.953575724842267
16	27.60169886823951	23.887265853239388	20.960891660324553	27.55014361819655
17	26.280902462376943	24.23342253209928	22.168757518473964	27.316917487049814
18	25.95684089067832	22.726045221319325	23.791520388873884	27.52559349912847
19	27.343922618024695	23.283332924164682	23.19740750742641	26.17533695038421
20	27.34883264183831	22.458448923477278	23.62703459111777	26.56568384356664
21	27.596788844425895	23.126212162128986	22.03127685169273	27.24572214175239
22	28.031325951930867	22.55419438784278	22.48790906635897	26.926570593867382
23	27.255542189379618	23.16549235263791	22.394618613900278	27.184346844082192
24	27.719539439766283	23.217047602680875	21.962536518302116	27.100876439250733
25	27.532958534848895	22.951906316745635	22.421623744875163	27.093511403530307
26	27.45194314192424	22.910171114329906	22.942086269118406	26.695799474627453
27	27.599243856332706	22.829155721405247	22.26204797093266	27.309552451329388
28	26.38892298627648	22.821790685684824	23.560749269633956	27.228537058404733
29	26.73262465322957	23.02310166204306	23.158127316917486	27.08614636780988
30	26.575503891193875	23.636854638744996	22.949451304838828	26.838190165222304
31	27.40529791569489	23.050106793017946	22.770235435641865	26.7743598556453
32	26.678614391279798	23.15321729310387	23.190042471705986	26.978125843910345
33	26.68106940318661	23.028011685856676	22.811970638057595	27.478948272899125
34	26.865195296197186	22.949451304838828	23.25387278128299	26.931480617680997
35	26.604964034075568	23.126212162128986	23.840620627010043	26.42820317678541
36	26.312817617165447	22.942086269118406	23.509194019590996	27.23590209412516
37	26.143421795595707	21.61637983944222	24.29970785358309	27.94049051137898
38	25.691699604743086	22.17121253038077	25.53212383080058	26.604964034075568
39	25.347997937789994	21.442073994058873	26.028036235975744	27.18189183217539
40	24.834900449267177	21.363513613041025	26.79645496280657	27.005130974885226
41	24.392998306041786	21.282498220116366	27.04932118920777	27.27518228463408
42	24.27761274642182	21.145017553335133	27.343922618024695	27.233447082218348
43	23.39871848378465	20.43060908845408	28.39957773795203	27.771094689809246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.0
4	0.0
5	0.0
6	3.5
7	7.0
8	5.5
9	4.0
10	5.0
11	6.0
12	6.0
13	11.5
14	17.0
15	25.0
16	33.0
17	36.0
18	39.0
19	39.0
20	49.0
21	59.0
22	70.0
23	81.0
24	107.0
25	133.0
26	133.0
27	163.5
28	194.0
29	282.0
30	370.0
31	441.0
32	512.0
33	512.0
34	633.5
35	755.0
36	904.5
37	1054.0
38	1290.5
39	1527.0
40	1527.0
41	1744.5
42	1962.0
43	2039.5
44	2117.0
45	2374.0
46	2631.0
47	2631.0
48	2798.5
49	2966.0
50	3215.5
51	3465.0
52	3705.5
53	3946.0
54	3946.0
55	3692.0
56	3438.0
57	3258.5
58	3079.0
59	2901.5
60	2724.0
61	2724.0
62	2602.5
63	2481.0
64	2261.0
65	2041.0
66	1790.0
67	1539.0
68	1539.0
69	1324.0
70	1109.0
71	1005.0
72	901.0
73	770.5
74	640.0
75	640.0
76	530.0
77	420.0
78	321.5
79	223.0
80	162.0
81	101.0
82	101.0
83	95.0
84	89.0
85	63.5
86	38.0
87	25.5
88	13.0
89	13.0
90	13.5
91	14.0
92	7.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	40733.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.88613654776226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.13686256574434	79.99165295951686
2	3.8617653784587236	6.633442172194536
3	1.2176995197804712	3.137505216900302
4	0.6202835581980334	2.1309503351091252
5	0.32586325177223874	1.3993567868804164
6	0.23153441573290648	1.1931357867085655
7	0.15435627715527098	0.9279945007733288
8	0.09432883603933226	0.6481231433972455
9	0.07717813857763549	0.5965678933542827
>10	0.28012805854104733	3.341271205165345
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.002455011906807748	0.0
18	0.0	0.0	0.0	0.002455011906807748	0.0
19	0.0	0.0	0.0	0.002455011906807748	0.0
20	0.0	0.0	0.0	0.002455011906807748	0.0
21	0.0	0.0	0.0	0.004910023813615496	0.0
22	0.0	0.0	0.0	0.004910023813615496	0.0
23	0.0	0.0	0.0	0.004910023813615496	0.0
24	0.0	0.0	0.0	0.009820047627230991	0.0
25	0.0	0.0	0.0	0.009820047627230991	0.0
26	0.0	0.0	0.0	0.014730071440846489	0.0
27	0.0	0.0	0.0	0.03682517860211622	0.0
28	0.0	0.0	0.0	0.08347040483146344	0.0
29	0.0	0.0	0.0	0.14730071440846487	0.0
30	0.0	0.0	0.0	0.26759629784204453	0.0
31	0.0	0.0	0.0	0.47627230992070313	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCATG	25	0.005435045	29.6	26
GGTATCA	25	0.005435045	29.6	1
TCTTATA	55	5.0269323E-4	20.181818	37
TCTGGAC	50	0.0069217687	18.5	3
CTCTTAT	85	0.00920251	13.058824	36
TGTCTCT	200	1.8589162E-7	12.025001	37
CTGTCTC	270	4.947651E-10	11.648149	37
CCTGTCT	180	1.1797928E-4	10.277778	36
>>END_MODULE