FastQCFastQC Report
Fri 10 Feb 2017
ERR1632066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605472
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.2122311188626394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8310.13724829554463294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8100.1337799270651657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT652.679366E-622.769231
TAATAGG1005.329639E-1022.22
GTCTTAC502.7000555E-422.21
AGTACAG502.7000555E-422.25
AGTCGGT603.722539E-521.58333211
CAAGATA705.095344E-621.1428574
CCGTCCA806.9489215E-720.81259
TAGGCGT450.003823747920.5555554
GGTATCA6200.020.2903231
ATTGCGT555.13923E-420.18181836
ACGAGCG656.895238E-519.9230775
GGATAGC759.253894E-619.73333524
CTAGCAC759.253894E-619.7333353
GGCTACG851.2433175E-619.5882341
GTAATAG1052.2540917E-819.380951
CTTATAC3350.019.32835837
GTACACT500.0070311318.54
AGACGGA1103.842615E-818.56
TAACCCG1205.158654E-918.528
AATTGCG701.2179884E-418.535
Produced by FastQC (version 0.11.5)