FastQCFastQC Report
Fri 10 Feb 2017
ERR1632065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660565
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.3250247893848448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20460.3097348481981334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14770.22359646666111588No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8770.13276513287867206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.11323639611544663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATC250.00549467229.63
GGTATCA9250.029.01
GCCGTCT1103.6379788E-1223.54545436
ATAGCAC603.7231985E-521.5833323
ATGCCGT1151.3460522E-1020.91304234
GCATTAG450.003824110820.5555571
TGACCCG450.003824110820.55555713
GATATGC555.1399117E-420.18181834
TATGCCG1202.382876E-1020.04166633
TGCCGTC1500.019.73333435
GTATCAA13700.019.5802922
CCGTCTT1409.276846E-1118.537
GTTAAGC609.231147E-418.53
ATACCGT500.00703179218.56
TAGAGTG1451.546141E-1017.8620685
CGAGCCG852.720278E-517.41176425
TCGTATG1452.9704097E-916.58620630
GAGCCGC904.4413515E-516.44444526
GGCCGCA1251.6544618E-716.2833
ATCCTAC803.3798406E-416.187525
Produced by FastQC (version 0.11.5)