##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632065.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660565
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.909080862594898	31.0	31.0	34.0	30.0	34.0
2	32.08558733811207	33.0	31.0	34.0	30.0	34.0
3	32.09995534126089	34.0	31.0	34.0	30.0	34.0
4	35.84680690015366	37.0	35.0	37.0	35.0	37.0
5	35.42483934207837	37.0	35.0	37.0	33.0	37.0
6	35.42773080620378	37.0	35.0	37.0	33.0	37.0
7	35.42805477129427	37.0	35.0	37.0	33.0	37.0
8	35.38309477492752	37.0	35.0	37.0	33.0	37.0
9	37.02838630566258	39.0	37.0	39.0	33.0	39.0
10	36.82677404948794	38.0	37.0	39.0	33.0	39.0
11	37.00641874758729	39.0	37.0	39.0	33.0	39.0
12	36.93800761469348	39.0	37.0	39.0	33.0	39.0
13	36.99894787038369	39.0	37.0	39.0	33.0	39.0
14	38.10771082331035	40.0	38.0	41.0	33.0	41.0
15	38.08907526132931	40.0	38.0	41.0	33.0	41.0
16	37.963634161664636	39.0	37.0	40.0	33.0	41.0
17	38.0170762907511	39.0	37.0	40.0	33.0	41.0
18	38.04707333873275	40.0	37.0	41.0	33.0	41.0
19	38.12649928470324	40.0	38.0	41.0	34.0	41.0
20	38.11224027915497	40.0	38.0	41.0	34.0	41.0
21	38.0791595073914	40.0	37.0	41.0	34.0	41.0
22	38.052597397682284	40.0	37.0	41.0	34.0	41.0
23	37.9915254365581	40.0	37.0	41.0	33.0	41.0
24	37.91806862307267	39.0	37.0	41.0	33.0	41.0
25	37.83618720337893	39.0	37.0	41.0	33.0	41.0
26	37.72397417362409	39.0	37.0	40.0	33.0	41.0
27	37.627617267036555	39.0	37.0	40.0	33.0	41.0
28	37.53735211523468	39.0	37.0	40.0	32.0	41.0
29	37.3867658746679	39.0	37.0	40.0	32.0	41.0
30	37.31827147971812	39.0	36.0	40.0	32.0	41.0
31	37.208466994164084	39.0	36.0	40.0	32.0	41.0
32	37.09869127186575	39.0	36.0	40.0	31.0	41.0
33	36.93896739912045	39.0	36.0	40.0	31.0	41.0
34	36.93148895263903	39.0	36.0	40.0	31.0	41.0
35	36.893471497884384	39.0	36.0	40.0	31.0	41.0
36	36.85421268156805	39.0	36.0	40.0	31.0	41.0
37	36.820833680258566	39.0	36.0	40.0	31.0	41.0
38	36.73019763384376	39.0	35.0	40.0	31.0	41.0
39	36.541259376442895	39.0	35.0	40.0	30.0	41.0
40	36.535836745816084	38.0	35.0	40.0	30.0	41.0
41	36.52098279503153	38.0	35.0	40.0	30.0	41.0
42	36.38316743999455	38.0	35.0	40.0	30.0	41.0
43	35.53469681257711	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	3.0
15	7.0
16	15.0
17	23.0
18	37.0
19	82.0
20	159.0
21	261.0
22	443.0
23	713.0
24	1183.0
25	1720.0
26	2536.0
27	3684.0
28	5343.0
29	7274.0
30	10077.0
31	13390.0
32	17831.0
33	24963.0
34	34904.0
35	51739.0
36	80717.0
37	129997.0
38	182256.0
39	91205.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.85457146533649	20.57435680061765	14.02299546600259	24.548076268043268
2	18.830243806438425	22.096992725924018	34.72648414614762	24.346279321489934
3	20.36135732289782	23.214218131448078	31.468364203371358	24.956060342282743
4	15.310983779037644	16.82332548651533	34.56510714312747	33.30058359131955
5	14.621422570072589	36.638786493380664	33.85859075185637	14.88120018469038
6	32.77391324093768	35.47281493872669	16.137397530901577	15.615874289434045
7	27.219274409028632	31.9903416015078	21.495386525171632	19.29499746429193
8	26.918622694208743	33.32798437701059	20.59509662183131	19.158296306949353
9	26.435702769598752	14.423864419095775	21.035023048450945	38.10540976285453
10	16.96789869278572	27.763808255054386	33.28983521682196	21.97845783533793
11	34.592659314374814	22.16012050290282	21.508405683013784	21.738814499708585
12	21.127973780021648	27.40790081218351	28.74297003322913	22.72115537456571
13	30.844655711398577	20.26613580798256	24.879762021905492	24.009446458713374
14	22.847108157410702	22.254887861149168	25.554184675240137	29.343819306199997
15	26.021057730881896	28.17512281153255	22.690575492192288	23.113243965393263
16	24.615291455042275	26.620695919402333	24.63890760182571	24.125105023729688
17	23.002126967066072	27.348103517443402	25.84242277444309	23.807346741047436
18	22.68921302218555	25.174509700029517	27.78258006403609	24.353697213748838
19	23.814007705524816	26.04013231097621	27.639672098885043	22.50618788461393
20	24.726711224482074	24.894446420866988	27.532642510578064	22.846199844072874
21	24.993149803577243	25.645924322360404	26.131720572540175	23.22920530152218
22	25.10093631966574	25.64864926237388	25.98200025735545	23.268414160604937
23	24.272554555569855	25.461990871450958	26.419353129517916	23.84610144346128
24	24.23304292537449	25.95232868831985	26.4375193962744	23.37710899003126
25	24.007327060925117	25.16209608441259	26.71924791655628	24.111328938106016
26	23.962668321815418	25.800337589790555	26.163814310476635	24.07317977791739
27	23.73392474623996	25.610348716628945	26.396191139403392	24.259535397727703
28	23.277194522870573	25.639414743439325	27.05517246599502	24.028218267695078
29	23.36817724220932	25.80745271093685	27.117240544079692	23.70712950277414
30	22.98199268807763	26.556962600198315	26.954955227721722	23.50608948400233
31	23.83868355120238	26.138078765904943	26.381052583772984	23.642185099119693
32	23.035734560565576	25.821077411004218	26.82612611930696	24.31706190912325
33	23.030738837207544	25.55615268747209	27.428792018953473	23.984316456366898
34	23.308077176356605	25.13923686541067	27.362636530848594	24.19004942738413
35	23.346983264326752	25.378426044371103	27.463610696903405	23.810979994398735
36	23.067222756276824	25.647135406810833	27.155995246493532	24.129646590418808
37	23.423735741372916	24.60060705608078	27.32024857508345	24.655408627462855
38	22.7726264637091	24.750932913490725	28.42733114833514	24.04910947446504
39	22.709347301173995	24.451795054233877	28.77930256674211	24.05955507785002
40	22.246864426665052	24.06122031896937	29.522454262638803	24.16946099172678
41	22.01373066995678	24.186416174032836	29.69488241126914	24.104970744741244
42	21.920628552829776	23.932391210554602	29.712443135800413	24.434537100815213
43	20.879701467682967	23.76283938749404	30.12012443892728	25.23733470589571
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	60.0
2	72.0
3	187.5
4	303.0
5	303.0
6	391.0
7	479.0
8	475.5
9	472.0
10	699.5
11	927.0
12	927.0
13	1603.5
14	2280.0
15	3517.5
16	4755.0
17	4614.5
18	4474.0
19	4474.0
20	5257.0
21	6040.0
22	6219.5
23	6399.0
24	7806.5
25	9214.0
26	9214.0
27	10682.0
28	12150.0
29	15816.0
30	19482.0
31	21106.5
32	22731.0
33	22731.0
34	24729.5
35	26728.0
36	29779.5
37	32831.0
38	34832.0
39	36833.0
40	36833.0
41	39731.5
42	42630.0
43	42720.5
44	42811.0
45	43430.5
46	44050.0
47	44050.0
48	45188.5
49	46327.0
50	46351.5
51	46376.0
52	47516.0
53	48656.0
54	48656.0
55	45706.5
56	42757.0
57	41037.5
58	39318.0
59	35768.0
60	32218.0
61	32218.0
62	29240.5
63	26263.0
64	23196.0
65	20129.0
66	17455.5
67	14782.0
68	14782.0
69	12418.5
70	10055.0
71	8703.0
72	7351.0
73	5764.0
74	4177.0
75	4177.0
76	3239.0
77	2301.0
78	1972.0
79	1643.0
80	1418.5
81	1194.0
82	1194.0
83	899.5
84	605.0
85	474.0
86	343.0
87	282.5
88	222.0
89	222.0
90	162.5
91	103.0
92	63.0
93	23.0
94	15.0
95	7.0
96	7.0
97	6.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	660565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.57102106536075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.41285248751419	59.741467178824536
2	7.24674194446042	9.793395051687368
3	1.6752539730406646	3.3959586450648014
4	0.723248913741256	1.9548267034363878
5	0.4027207347055305	1.360612562412248
6	0.2729990572031729	1.1068095027059532
7	0.17947949460913282	0.8489328897723811
8	0.13312397291069125	0.7196258222282267
9	0.09173317333808902	0.5578653769218362
>10	0.7212248037638833	9.692873002811996
>50	0.0949476904794501	4.482938118054576
>100	0.04387380332787653	4.972922584741748
>500	0.0011249693160689806	0.5097587718238005
>1k	6.749815896413884E-4	0.8620137895141511
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2147	0.3250247893848448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2046	0.3097348481981334	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1477	0.22359646666111588	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	877	0.13276513287867206	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.11323639611544663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5138555630407304E-4	0.0
3	0.0	0.0	0.0	1.5138555630407304E-4	0.0
4	0.0	0.0	0.0	1.5138555630407304E-4	0.0
5	0.0	0.0	0.0	1.5138555630407304E-4	0.0
6	0.0	0.0	0.0	1.5138555630407304E-4	0.0
7	0.0	0.0	0.0	1.5138555630407304E-4	0.0
8	0.0	0.0	0.0	1.5138555630407304E-4	0.0
9	0.0	0.0	0.0	1.5138555630407304E-4	0.0
10	0.0	0.0	0.0	1.5138555630407304E-4	0.0
11	0.0	0.0	0.0	1.5138555630407304E-4	0.0
12	0.0	0.0	0.0	1.5138555630407304E-4	0.0
13	0.0	0.0	0.0	1.5138555630407304E-4	0.0
14	0.0	0.0	0.0	1.5138555630407304E-4	0.0
15	0.0	0.0	0.0	1.5138555630407304E-4	0.0
16	0.0	0.0	0.0	6.055422252162922E-4	0.0
17	0.0	0.0	0.0	0.0010596988941285112	0.0
18	0.0	0.0	0.0	0.0012110844504325843	0.0
19	0.0	0.0	0.0	0.0015138555630407303	0.0
20	0.0	0.0	0.0	0.0019680122319529494	0.0
21	0.0	0.0	0.0	0.0027249400134733144	0.0
22	0.0	0.0	0.0	0.0037846389076018256	0.0
23	0.0	0.0	0.0	0.005147108914338483	0.0
24	0.0	0.0	0.0	0.007417892258899579	0.0
25	0.0	0.0	0.0	0.009385904490852527	0.0
26	0.0	0.0	0.0	0.011808073391717697	0.0
27	0.0	0.0	0.0	0.025886930127996487	0.0
28	0.0	0.0	0.0	0.07054566923769803	0.0
29	0.0	0.0	0.0	0.13685254289888202	0.0
30	0.0	0.0	0.0	0.23328514226457653	0.0
31	0.0	0.0	0.0	0.5240967959247008	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGATC	25	0.005494672	29.6	3
GGTATCA	925	0.0	29.0	1
GCCGTCT	110	3.6379788E-12	23.545454	36
ATAGCAC	60	3.7231985E-5	21.583332	3
ATGCCGT	115	1.3460522E-10	20.913042	34
GCATTAG	45	0.0038241108	20.555557	1
TGACCCG	45	0.0038241108	20.555557	13
GATATGC	55	5.1399117E-4	20.181818	34
TATGCCG	120	2.382876E-10	20.041666	33
TGCCGTC	150	0.0	19.733334	35
GTATCAA	1370	0.0	19.580292	2
CCGTCTT	140	9.276846E-11	18.5	37
GTTAAGC	60	9.231147E-4	18.5	3
ATACCGT	50	0.007031792	18.5	6
TAGAGTG	145	1.546141E-10	17.862068	5
CGAGCCG	85	2.720278E-5	17.411764	25
TCGTATG	145	2.9704097E-9	16.586206	30
GAGCCGC	90	4.4413515E-5	16.444445	26
GGCCGCA	125	1.6544618E-7	16.28	33
ATCCTAC	80	3.3798406E-4	16.1875	25
>>END_MODULE