FastQCFastQC Report
Fri 10 Feb 2017
ERR1632064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639605
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG250.00549454329.615
GGTATCA3450.024.6666681
TTAGACT759.255233E-619.7333354
CTTATAC5100.019.2254937
GCATTAG500.007031553418.51
GCGAAAG1258.556526E-917.7618
GTATCAA4800.017.7291682
AACGAAC752.0654598E-417.26666814
GAGCGAA1401.8644641E-917.17857216
ACGGACC1401.8644641E-917.1785728
CCTAGTC650.001578898317.0769238
TAGACTT650.001578898317.0769235
CATTAGA650.001578898317.0769232
TCTTATA9450.017.03174637
AGCGAAA1352.2137101E-816.44444517
GGTTATC803.3796308E-416.18752
CGGACCA1504.656613E-916.0333359
AAGACGG1751.3096724E-1015.8571435
TCGCGGA700.002590808315.85714232
TAACGCC1201.9321378E-615.4166674