##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632064.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639605
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.084361441827376	33.0	31.0	34.0	30.0	34.0
2	32.2843927111264	34.0	31.0	34.0	30.0	34.0
3	32.37829128915502	34.0	31.0	34.0	30.0	34.0
4	35.99415733147802	37.0	35.0	37.0	35.0	37.0
5	35.620117103524834	37.0	35.0	37.0	33.0	37.0
6	35.60375700627731	37.0	35.0	37.0	33.0	37.0
7	35.5903049538387	37.0	35.0	37.0	33.0	37.0
8	35.558669804019665	37.0	35.0	37.0	33.0	37.0
9	37.25274505358776	39.0	37.0	39.0	34.0	39.0
10	37.11658445446799	39.0	37.0	39.0	33.0	39.0
11	37.216081800486236	39.0	37.0	39.0	34.0	39.0
12	37.15993621063	39.0	37.0	39.0	34.0	39.0
13	37.20551434088226	39.0	37.0	39.0	34.0	39.0
14	38.405207901751865	40.0	38.0	41.0	34.0	41.0
15	38.379135560228576	40.0	38.0	41.0	34.0	41.0
16	38.35717513152649	40.0	38.0	41.0	34.0	41.0
17	38.31893277882443	40.0	38.0	41.0	34.0	41.0
18	38.31676268947241	40.0	38.0	41.0	34.0	41.0
19	38.34265054213147	40.0	38.0	41.0	34.0	41.0
20	38.31304164288897	40.0	38.0	41.0	34.0	41.0
21	38.28600308002596	40.0	38.0	41.0	34.0	41.0
22	38.23491842621618	40.0	38.0	41.0	34.0	41.0
23	38.17848203187905	40.0	38.0	41.0	34.0	41.0
24	38.113141704645834	40.0	38.0	41.0	34.0	41.0
25	38.036555374019905	40.0	37.0	41.0	33.0	41.0
26	37.917092580577076	40.0	37.0	41.0	33.0	41.0
27	37.81591607320143	40.0	37.0	41.0	33.0	41.0
28	37.72889205056246	39.0	37.0	41.0	33.0	41.0
29	37.64242149451615	39.0	37.0	40.0	33.0	41.0
30	37.56361660712471	39.0	37.0	40.0	33.0	41.0
31	37.4737846014337	39.0	37.0	40.0	32.0	41.0
32	37.35756287083434	39.0	37.0	40.0	32.0	41.0
33	37.26441944637706	39.0	36.0	40.0	32.0	41.0
34	37.178200608187865	39.0	36.0	40.0	31.0	41.0
35	37.147880332392646	39.0	36.0	40.0	31.0	41.0
36	37.109825595484715	39.0	36.0	40.0	31.0	41.0
37	37.054729090610614	39.0	36.0	40.0	31.0	41.0
38	36.99896342273747	39.0	36.0	40.0	31.0	41.0
39	36.93946576402624	39.0	36.0	40.0	31.0	41.0
40	36.855751596688584	39.0	35.0	40.0	31.0	41.0
41	36.81913524753559	39.0	35.0	40.0	31.0	41.0
42	36.75558665113625	39.0	35.0	40.0	31.0	41.0
43	35.826100483892404	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	5.0
16	3.0
17	16.0
18	31.0
19	59.0
20	106.0
21	183.0
22	372.0
23	566.0
24	1003.0
25	1485.0
26	2182.0
27	3266.0
28	4478.0
29	6325.0
30	8601.0
31	11675.0
32	15486.0
33	20996.0
34	30146.0
35	44428.0
36	69482.0
37	117579.0
38	188312.0
39	112818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28547306540756	18.538003924297026	12.469102023905378	26.70742098639004
2	20.25187420361004	19.883365514653576	33.154212365444295	26.71054791629209
3	21.143987304664595	21.277194518491882	28.354844005284512	29.223974171559007
4	16.423261231541343	15.43812196590083	32.29024163350818	35.848375169049646
5	17.07569515560385	33.80946052641865	32.29915338372902	16.81569093424848
6	36.58617427943809	32.82948069511652	14.840409315124178	15.743935710321214
7	30.71864666473839	28.41675721734508	20.34208613128415	20.522509986632375
8	27.713823375364484	32.0998116024734	19.233433134512705	20.95293188764941
9	27.08734296948898	13.962680091619045	19.235778331939244	39.71419860695273
10	18.418242509048554	25.30968332017417	31.471767731646878	24.800306439130402
11	38.061616153719875	20.285957739542372	20.176827885960865	21.475598220776888
12	23.009044644741678	23.917261434791786	27.69959584431016	25.374098076156375
13	31.80306595476896	18.582406328906124	22.86473682976212	26.749790886562803
14	23.99887430523526	19.653223473862774	23.270299638057864	33.0776025828441
15	26.693193455335717	26.211333557429978	20.62257174349794	26.47290124373637
16	26.755888399871797	24.5765746046388	22.535314764581265	26.132222230908138
17	25.491357947483213	24.78232659219362	23.82486065618624	25.901454804136932
18	25.781224349403146	23.77889478662612	24.663346909420657	25.776533954550075
19	26.275904659907283	24.051250381094583	24.249966776369792	25.422878182628338
20	26.282783905691794	23.791246159739213	24.023420704966348	25.902549229602645
21	26.855168424261848	23.84409127508384	23.62176655904816	25.67897374160615
22	26.938501106151453	23.68743208699119	23.518890565270752	25.8551762415866
23	26.139883209168158	23.7071317453741	23.698376341648363	26.454608703809384
24	26.127531836055066	24.050624995114173	23.618795975641216	26.203047193189548
25	26.284034677652613	23.46510737095551	23.79218423870983	26.458673712682046
26	26.034662017964212	24.16866659891652	23.95087593123881	25.84579545188046
27	26.229000711376553	23.615825392234267	23.8414333846671	26.313740511722077
28	25.814213459869762	23.8093823531711	24.31313076039118	26.063273426567964
29	25.433666090790407	24.004033739573643	24.546243384588927	26.01605678504702
30	25.50449105307182	24.229641732006474	24.091118737345703	26.174748477576003
31	26.151140156815533	23.910225842512176	23.71182214022717	26.22681186044512
32	25.315780833483164	23.886930214741913	24.118166680998428	26.679122270776496
33	25.270596696398556	23.51232401247645	24.580170574026155	26.636908717098834
34	26.115493155932178	23.128493366999944	24.447119706693975	26.308893770373903
35	25.802800165727284	23.112702370994597	25.028728668475075	26.05576879480304
36	25.185387856567726	23.05078915893403	25.02263115516608	26.741191829332166
37	25.12097310058552	22.420712783671174	25.687729145331883	26.770584970411427
38	24.513723313607617	22.224028892832294	26.74791472862157	26.514333064938516
39	24.46087819826299	21.664621133355745	27.230087319517516	26.64441334886375
40	24.173356993769595	21.82300013289452	27.76479233276788	26.23885054056801
41	23.436652308846867	21.9685587198349	28.265570156581017	26.32921881473722
42	23.305477599455916	22.289694420775323	28.280423073615747	26.124404906153014
43	22.711829957551927	21.858334440787676	28.564661001711993	26.865174599948404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	24.5
2	23.0
3	60.5
4	98.0
5	98.0
6	144.0
7	190.0
8	180.5
9	171.0
10	250.0
11	329.0
12	329.0
13	597.0
14	865.0
15	1214.5
16	1564.0
17	1572.5
18	1581.0
19	1581.0
20	2032.5
21	2484.0
22	2649.0
23	2814.0
24	3378.5
25	3943.0
26	3943.0
27	4929.0
28	5915.0
29	7257.0
30	8599.0
31	10217.0
32	11835.0
33	11835.0
34	14275.5
35	16716.0
36	19382.5
37	22049.0
38	24680.5
39	27312.0
40	27312.0
41	29735.0
42	32158.0
43	34489.5
44	36821.0
45	38556.5
46	40292.0
47	40292.0
48	43054.0
49	45816.0
50	47083.5
51	48351.0
52	49992.0
53	51633.0
54	51633.0
55	51079.5
56	50526.0
57	49427.0
58	48328.0
59	46584.5
60	44841.0
61	44841.0
62	41699.5
63	38558.0
64	34565.0
65	30572.0
66	26487.0
67	22402.0
68	22402.0
69	18883.5
70	15365.0
71	13296.5
72	11228.0
73	8911.0
74	6594.0
75	6594.0
76	5288.5
77	3983.0
78	3147.0
79	2311.0
80	1903.0
81	1495.0
82	1495.0
83	1140.0
84	785.0
85	651.0
86	517.0
87	425.5
88	334.0
89	334.0
90	243.5
91	153.0
92	87.5
93	22.0
94	14.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	639605.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.48375458718802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91821430000424	64.451260236525
2	6.401152796441423	9.279591767447048
3	1.8386635060295493	3.9981970301839365
4	0.8442128554571459	2.4476686973721997
5	0.49333923678942515	1.7879540083837666
6	0.2934017157814522	1.2760114777297602
7	0.21253712213773718	1.0783842021189263
8	0.1503105810685919	0.8716060216026756
9	0.12109164994828242	0.7899459693668084
>10	0.6519991776129922	8.625039209009755
>50	0.05171020735215804	2.6136656148915414
>100	0.022934131907138847	2.5887211583740846
>500	4.32719469907219E-4	0.1919546069945401
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.5634649510244604E-4	0.0	0.0	0.0
6	0.0	1.5634649510244604E-4	0.0	0.0	0.0
7	0.0	1.5634649510244604E-4	0.0	0.0	0.0
8	0.0	1.5634649510244604E-4	0.0	0.0	0.0
9	0.0	1.5634649510244604E-4	0.0	0.0	0.0
10	0.0	1.5634649510244604E-4	0.0	0.0	0.0
11	0.0	1.5634649510244604E-4	0.0	0.0	0.0
12	0.0	1.5634649510244604E-4	0.0	0.0	0.0
13	0.0	1.5634649510244604E-4	0.0	0.0	0.0
14	0.0	1.5634649510244604E-4	0.0	0.0	0.0
15	0.0	1.5634649510244604E-4	0.0	1.5634649510244604E-4	0.0
16	0.0	1.5634649510244604E-4	0.0	3.126929902048921E-4	0.0
17	0.0	1.5634649510244604E-4	0.0	7.817324755122302E-4	0.0
18	0.0	1.5634649510244604E-4	0.0	9.380789706146763E-4	0.0
19	0.0	1.5634649510244604E-4	0.0	0.0018761579412293525	0.0
20	0.0	1.5634649510244604E-4	0.0	0.0025015439216391367	0.0
21	0.0	1.5634649510244604E-4	0.0	0.0034396228922538128	0.0
22	0.0	1.5634649510244604E-4	0.0	0.0034396228922538128	0.0
23	0.0	1.5634649510244604E-4	0.0	0.0048467413481758275	0.0
24	0.0	1.5634649510244604E-4	0.0	0.0068792457845076255	0.0
25	0.0	1.5634649510244604E-4	0.0	0.007817324755122302	0.0
26	0.0	1.5634649510244604E-4	0.0	0.0118823336277859	0.0
27	0.0	1.5634649510244604E-4	0.0	0.03220737799110388	0.0
28	0.0	1.5634649510244604E-4	0.0	0.10443945872843395	0.0
29	0.0	1.5634649510244604E-4	0.0	0.1973092768192869	0.0
30	0.0	1.5634649510244604E-4	0.0	0.32004127547470707	0.0
31	0.0	1.5634649510244604E-4	0.0	0.6946474777401678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	25	0.005494543	29.6	15
GGTATCA	345	0.0	24.666668	1
TTAGACT	75	9.255233E-6	19.733335	4
CTTATAC	510	0.0	19.22549	37
GCATTAG	50	0.0070315534	18.5	1
GCGAAAG	125	8.556526E-9	17.76	18
GTATCAA	480	0.0	17.729168	2
AACGAAC	75	2.0654598E-4	17.266668	14
GAGCGAA	140	1.8644641E-9	17.178572	16
ACGGACC	140	1.8644641E-9	17.178572	8
CCTAGTC	65	0.0015788983	17.076923	8
TAGACTT	65	0.0015788983	17.076923	5
CATTAGA	65	0.0015788983	17.076923	2
TCTTATA	945	0.0	17.031746	37
AGCGAAA	135	2.2137101E-8	16.444445	17
GGTTATC	80	3.3796308E-4	16.1875	2
CGGACCA	150	4.656613E-9	16.033335	9
AAGACGG	175	1.3096724E-10	15.857143	5
TCGCGGA	70	0.0025908083	15.857142	32
TAACGCC	120	1.9321378E-6	15.416667	4
>>END_MODULE