FastQCFastQC Report
Fri 10 Feb 2017
ERR1632063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721454
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG15600.2162300021900218No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA14230.19724057251051347No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT14050.19474561094678247No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13830.19169621348000013No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC12820.17769670692795383No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA12440.1724295658489661No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC12140.1682712965760811No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC11720.16244971959404203No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT11690.16203389266675353No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC11680.16189528369099068No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA11010.15260848231488078No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG10960.1519154374360666No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG10910.15122239255725245No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA10620.14720273226013023No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT10580.1466482963570789No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG10540.14609386045402756No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG10130.14041089244775135No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA10090.13985645654470003No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA10020.13888619371436015No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC9910.137361494980969No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG9860.1366684501021548No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA9500.13167852697469276No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9470.13126270004740426No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC8990.12460946921078822No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG8740.12114424481671736No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA8680.12031259096214034No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG8440.1169859755438323No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC8070.11185744344060745No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC8030.11130300753755609No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT8000.11088718061026759No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG7950.11019413573145342No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC7900.10950109085263926No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG7830.10853082802229942No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC7570.1049269946524657No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG7470.10354090489483737No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC7460.10340229591907454No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC7450.1032636869433117No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT7270.10076872537958068No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC7230.10021428947652934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.024.8507441
CTTATAC5700.019.14912237
CGTGCGT701.2183987E-418.514
GTACTAT609.2322717E-418.56
GTATTAT500.007032406518.4999981
GAGTATA500.007032406518.4999981
CACCTAT500.007032406518.4999981
GAGCGTC500.007032406518.49999811
CCCATAG902.1499582E-618.4999981
ATACTGG752.0659229E-417.2666666
GGACCAT752.0659229E-417.2666666
GCATTCG650.00157916317.07692322
TGCGTAC650.00157916317.07692316
GTATCAA5000.016.2799992
CTAAAGT803.3803825E-416.18754
GTACAAC700.002591238815.8571431
ATTCGTA700.002591238815.85714324
AGTTGTC700.002591238815.8571438
GTGCGTA700.002591238815.85714315
TACCGGC700.002591238815.8571437
Produced by FastQC (version 0.11.5)