##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632063.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721454
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.206890529403125	33.0	31.0	34.0	30.0	34.0
2	32.391595306145646	34.0	31.0	34.0	31.0	34.0
3	32.4882528893041	34.0	31.0	34.0	31.0	34.0
4	36.060627565998665	37.0	35.0	37.0	35.0	37.0
5	35.784242654417326	37.0	35.0	37.0	35.0	37.0
6	35.77327729834473	37.0	35.0	37.0	35.0	37.0
7	35.74970129765723	37.0	35.0	37.0	35.0	37.0
8	35.72903469937099	37.0	35.0	37.0	33.0	37.0
9	37.4765057786082	39.0	37.0	39.0	35.0	39.0
10	37.34573929869403	39.0	37.0	39.0	34.0	39.0
11	37.44139058068844	39.0	37.0	39.0	35.0	39.0
12	37.35817252381995	39.0	37.0	39.0	35.0	39.0
13	37.41588791523784	39.0	37.0	39.0	35.0	39.0
14	38.6658497977695	40.0	38.0	41.0	35.0	41.0
15	38.63298422352638	40.0	38.0	41.0	35.0	41.0
16	38.64119541925057	40.0	38.0	41.0	35.0	41.0
17	38.577810920723984	40.0	38.0	41.0	35.0	41.0
18	38.57095947905203	40.0	38.0	41.0	35.0	41.0
19	38.583039251289755	40.0	38.0	41.0	34.0	41.0
20	38.560512520548784	40.0	38.0	41.0	34.0	41.0
21	38.536792366526484	40.0	38.0	41.0	34.0	41.0
22	38.49029875778636	40.0	38.0	41.0	34.0	41.0
23	38.44682682471786	40.0	38.0	41.0	34.0	41.0
24	38.38980309208903	40.0	38.0	41.0	34.0	41.0
25	38.33038974071805	40.0	38.0	41.0	34.0	41.0
26	38.20401300706629	40.0	38.0	41.0	34.0	41.0
27	38.10803460788907	40.0	38.0	41.0	34.0	41.0
28	38.01905734807763	40.0	38.0	41.0	33.0	41.0
29	37.943130123334264	40.0	37.0	41.0	33.0	41.0
30	37.87673087958484	40.0	37.0	41.0	33.0	41.0
31	37.75451795956499	40.0	37.0	41.0	33.0	41.0
32	37.65599331350301	39.0	37.0	41.0	33.0	41.0
33	37.57331444555024	39.0	37.0	41.0	33.0	41.0
34	37.44736739972333	39.0	37.0	41.0	32.0	41.0
35	37.44755590793037	39.0	37.0	41.0	32.0	41.0
36	37.38823819675267	39.0	36.0	40.0	32.0	41.0
37	37.30338594005993	39.0	36.0	40.0	32.0	41.0
38	37.27818405608674	39.0	36.0	40.0	32.0	41.0
39	37.18241911473219	39.0	36.0	40.0	32.0	41.0
40	37.08824124615013	39.0	36.0	40.0	31.0	41.0
41	37.02030205667998	39.0	35.0	40.0	31.0	41.0
42	36.96880466391482	39.0	35.0	40.0	31.0	41.0
43	36.02695805969611	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	5.0
17	11.0
18	19.0
19	49.0
20	74.0
21	153.0
22	275.0
23	492.0
24	913.0
25	1414.0
26	2124.0
27	3128.0
28	4372.0
29	6276.0
30	8874.0
31	11911.0
32	15519.0
33	21350.0
34	29878.0
35	44075.0
36	69914.0
37	122240.0
38	218127.0
39	160258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.379683250768586	17.693851583053114	11.457417936555899	26.4690472296224
2	20.514682848802558	19.445591818743814	32.81234839643276	27.22737693602087
3	22.188386231138786	19.683722039104364	27.369312527202013	30.75857920255484
4	15.546659939511043	15.155644018884088	31.773058296163025	37.524637745441844
5	17.937941989371463	33.02303403959227	31.61781624330865	17.421207727727616
6	38.50307850535169	31.23442381634865	14.844051041369235	15.418446636930422
7	32.895790999841985	27.004632311969996	19.092693366451638	21.00688332173638
8	28.52531138506405	30.967185711077903	19.076891943214676	21.430610960643367
9	27.4469335536292	12.733452167428554	18.36652094243015	41.4530933365121
10	18.224030915345953	24.727979885065437	31.591896364841	25.456092834747608
11	38.98834852949737	19.83951852786179	18.98568723716273	22.186445705478103
12	23.413828185858005	23.048732143698697	26.55346009586197	26.98397957458133
13	32.97244176343883	17.61054758861965	22.481266996925655	26.935743651015866
14	25.31609776922714	19.068298186717378	22.38132992540065	33.234274118654824
15	26.773848367324877	25.502249623676633	20.627649164049267	27.096252844949227
16	28.823320682954147	22.958081873549805	21.843665708416616	26.37493173507944
17	26.384495754407073	23.679957419322644	22.79590937190729	27.139637454362997
18	25.88370152497595	22.15248650641621	24.462266478528083	27.501545490079753
19	28.380187787440363	23.468994558211612	23.267179889500923	24.8836377648471
20	28.21621336911293	22.726882101977395	22.910954821790437	26.145949707119232
21	27.075877325512092	23.34424648002506	22.559026632328603	27.02084956213425
22	28.64992085427484	22.212365583945754	22.53199788205485	26.605715679724558
23	25.587909970698064	22.937706354112667	23.17417326676406	28.300210408425208
24	26.670723289357323	23.523052058759117	22.663260582102254	27.1429640697813
25	27.303611872690425	22.689319069545668	23.01019884843663	26.996870209327273
26	27.651936228782432	23.236270087905815	21.797647528463354	27.3141461548484
27	27.863869352723803	22.668804941132766	21.954137062099594	27.513188644043833
28	26.641892622398654	23.35242440959507	23.030019931970717	26.975663036035563
29	27.281434436568375	23.45416339780499	23.590970456882907	25.67343170874373
30	27.27367233392566	22.527978221757728	22.31632231576788	27.882027128548735
31	26.011776218580813	22.541700510358247	23.273555902386015	28.17296736867493
32	25.779467575202297	23.1945487862012	23.22143892749919	27.80454471109731
33	26.531144050764148	22.185059615720476	23.695343015632318	27.588453317883054
34	27.77654569799322	22.345568809653837	23.730411086500318	26.147474405852627
35	27.69823162668722	21.809152073451667	23.071602624699565	27.42101367516155
36	25.798872831809096	22.177297513077757	23.06411774000837	28.959711915104773
37	25.21422017204146	21.729867739315328	26.032844782896763	27.02306730574645
38	25.350334186240563	21.06496048258101	24.90415189326	28.680553437918427
39	24.641349275213667	21.56076478888467	27.26660327616175	26.53128265973991
40	26.135138207009735	20.596184926551103	26.229530919504224	27.03914594693494
41	23.927790267986595	21.601515827758945	27.22793137192392	27.242762532330545
42	23.61328650198072	22.2938676616943	27.045660568795792	27.047185267529184
43	23.824665190019047	20.933697782533606	26.84855860526104	28.393078422186306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	18.5
2	15.0
3	44.5
4	74.0
5	74.0
6	98.5
7	123.0
8	118.5
9	114.0
10	175.0
11	236.0
12	236.0
13	377.5
14	519.0
15	783.0
16	1047.0
17	1135.5
18	1224.0
19	1224.0
20	1546.5
21	1869.0
22	2142.0
23	2415.0
24	2999.5
25	3584.0
26	3584.0
27	4168.0
28	4752.0
29	5960.0
30	7168.0
31	8847.0
32	10526.0
33	10526.0
34	14423.5
35	18321.0
36	20470.5
37	22620.0
38	24106.0
39	25592.0
40	25592.0
41	27255.0
42	28918.0
43	32859.0
44	36800.0
45	37919.5
46	39039.0
47	39039.0
48	40561.5
49	42084.0
50	46270.5
51	50457.0
52	57134.5
53	63812.0
54	63812.0
55	63024.0
56	62236.0
57	57461.5
58	52687.0
59	54673.5
60	56660.0
61	56660.0
62	54006.5
63	51353.0
64	42528.0
65	33703.0
66	31369.0
67	29035.0
68	29035.0
69	26413.5
70	23792.0
71	24353.5
72	24915.0
73	20625.5
74	16336.0
75	16336.0
76	10151.5
77	3967.0
78	3126.0
79	2285.0
80	1876.0
81	1467.0
82	1467.0
83	1120.5
84	774.0
85	631.5
86	489.0
87	387.0
88	285.0
89	285.0
90	201.0
91	117.0
92	68.0
93	19.0
94	11.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	721454.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.71316576036921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.72477982344135	50.88576310939461
2	5.740414712348478	6.511141822293628
3	1.5789057439787553	2.6863422953479867
4	0.7086885744527365	1.607678903816712
5	0.41844971304482537	1.1865803969145057
6	0.29997039119057745	1.020736231127641
7	0.2156661177670478	0.8561775800072523
8	0.14936481470560528	0.6776761196132661
9	0.12192766349271766	0.6223413411393314
>10	0.8225181224878836	9.259028931762415
>50	0.10184199788455486	4.050387618036205
>100	0.09891120937266887	12.204480450588576
>500	0.01392083687465255	5.314407590261718
>1k	0.004640278958217517	3.117257609696114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1560	0.2162300021900218	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1423	0.19724057251051347	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1405	0.19474561094678247	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1383	0.19169621348000013	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	1282	0.17769670692795383	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	1244	0.1724295658489661	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	1214	0.1682712965760811	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	1172	0.16244971959404203	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	1169	0.16203389266675353	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1168	0.16189528369099068	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	1101	0.15260848231488078	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	1096	0.1519154374360666	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	1091	0.15122239255725245	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	1062	0.14720273226013023	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1058	0.1466482963570789	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1054	0.14609386045402756	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1013	0.14041089244775135	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	1009	0.13985645654470003	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	1002	0.13888619371436015	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	991	0.137361494980969	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	986	0.1366684501021548	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	950	0.13167852697469276	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	947	0.13126270004740426	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	899	0.12460946921078822	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	874	0.12114424481671736	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	868	0.12031259096214034	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	844	0.1169859755438323	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	807	0.11185744344060745	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	803	0.11130300753755609	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	800	0.11088718061026759	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	795	0.11019413573145342	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	790	0.10950109085263926	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	783	0.10853082802229942	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	757	0.1049269946524657	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	747	0.10354090489483737	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	746	0.10340229591907454	No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC	745	0.1032636869433117	No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT	727	0.10076872537958068	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	723	0.10021428947652934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.386089757628345E-4	0.0
8	0.0	0.0	0.0	1.386089757628345E-4	0.0
9	0.0	0.0	0.0	1.386089757628345E-4	0.0
10	1.386089757628345E-4	0.0	0.0	1.386089757628345E-4	0.0
11	1.386089757628345E-4	0.0	0.0	1.386089757628345E-4	0.0
12	1.386089757628345E-4	0.0	0.0	1.386089757628345E-4	0.0
13	1.386089757628345E-4	0.0	0.0	1.386089757628345E-4	0.0
14	1.386089757628345E-4	0.0	0.0	1.386089757628345E-4	0.0
15	1.386089757628345E-4	0.0	0.0	6.930448788141724E-4	0.0
16	1.386089757628345E-4	0.0	0.0	9.702628303398415E-4	0.0
17	1.386089757628345E-4	0.0	0.0	0.0012474807818655105	0.0
18	1.386089757628345E-4	0.0	0.0	0.0012474807818655105	0.0
19	1.386089757628345E-4	0.0	0.0	0.001386089757628345	0.0
20	1.386089757628345E-4	0.0	0.0	0.0023563525879681867	0.0
21	1.386089757628345E-4	0.0	0.0	0.00277217951525669	0.0
22	1.386089757628345E-4	0.0	0.0	0.0034652243940708626	0.0
23	1.386089757628345E-4	0.0	0.0	0.004574096200173538	0.0
24	1.386089757628345E-4	0.0	0.0	0.007207666739667394	0.0
25	1.386089757628345E-4	0.0	0.0	0.009148192400347076	0.0
26	1.386089757628345E-4	0.0	0.0	0.013999506552046284	0.0
27	1.386089757628345E-4	0.0	0.0	0.034236417013420124	0.0
28	1.386089757628345E-4	0.0	0.0	0.1028478600160232	0.0
29	1.386089757628345E-4	0.0	0.0	0.1980722263650905	0.0
30	1.386089757628345E-4	0.0	0.0	0.30715749029044126	0.0
31	1.386089757628345E-4	0.0	0.0	0.7034405519963851	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	335	0.0	24.850744	1
CTTATAC	570	0.0	19.149122	37
CGTGCGT	70	1.2183987E-4	18.5	14
GTACTAT	60	9.2322717E-4	18.5	6
GTATTAT	50	0.0070324065	18.499998	1
GAGTATA	50	0.0070324065	18.499998	1
CACCTAT	50	0.0070324065	18.499998	1
GAGCGTC	50	0.0070324065	18.499998	11
CCCATAG	90	2.1499582E-6	18.499998	1
ATACTGG	75	2.0659229E-4	17.266666	6
GGACCAT	75	2.0659229E-4	17.266666	6
GCATTCG	65	0.001579163	17.076923	22
TGCGTAC	65	0.001579163	17.076923	16
GTATCAA	500	0.0	16.279999	2
CTAAAGT	80	3.3803825E-4	16.1875	4
GTACAAC	70	0.0025912388	15.857143	1
ATTCGTA	70	0.0025912388	15.857143	24
AGTTGTC	70	0.0025912388	15.857143	8
GTGCGTA	70	0.0025912388	15.857143	15
TACCGGC	70	0.0025912388	15.857143	7
>>END_MODULE