##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37316
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45977596741344	34.0	31.0	34.0	31.0	34.0
2	32.64511201629328	34.0	31.0	34.0	31.0	34.0
3	32.717922606924645	34.0	31.0	34.0	31.0	34.0
4	36.20554185872012	37.0	37.0	37.0	35.0	37.0
5	36.00943295101297	37.0	35.0	37.0	35.0	37.0
6	36.04909422231751	37.0	35.0	37.0	35.0	37.0
7	36.01189838139136	37.0	35.0	37.0	35.0	37.0
8	35.99884767927966	37.0	35.0	37.0	35.0	37.0
9	37.72730196162504	39.0	37.0	39.0	35.0	39.0
10	37.66805123807482	39.0	37.0	39.0	35.0	39.0
11	37.74533712080609	39.0	37.0	39.0	35.0	39.0
12	37.70053060349448	39.0	37.0	39.0	35.0	39.0
13	37.73201843713152	39.0	37.0	39.0	35.0	39.0
14	39.05539178904491	40.0	38.0	41.0	36.0	41.0
15	39.06946082109551	40.0	38.0	41.0	36.0	41.0
16	39.03060349447958	40.0	38.0	41.0	36.0	41.0
17	39.01436381176975	40.0	38.0	41.0	36.0	41.0
18	38.999785614749705	40.0	38.0	41.0	36.0	41.0
19	39.021224139779186	40.0	39.0	41.0	36.0	41.0
20	39.02610140422339	40.0	38.0	41.0	36.0	41.0
21	38.96553757101511	40.0	38.0	41.0	36.0	41.0
22	38.94980705327473	40.0	38.0	41.0	36.0	41.0
23	38.89382570479151	40.0	38.0	41.0	35.0	41.0
24	38.85510236895701	40.0	38.0	41.0	35.0	41.0
25	38.77942437560296	40.0	38.0	41.0	35.0	41.0
26	38.67408082323936	40.0	38.0	41.0	35.0	41.0
27	38.56273448386751	40.0	38.0	41.0	35.0	41.0
28	38.493675635116304	40.0	38.0	41.0	34.0	41.0
29	38.4406956801372	40.0	38.0	41.0	34.0	41.0
30	38.35218136992175	40.0	38.0	41.0	34.0	41.0
31	38.27886161432094	40.0	38.0	41.0	34.0	41.0
32	38.20597062922071	40.0	38.0	41.0	34.0	41.0
33	38.13720656018866	40.0	38.0	41.0	34.0	41.0
34	38.0368742630507	40.0	38.0	41.0	33.0	41.0
35	38.0046896773502	40.0	38.0	41.0	33.0	41.0
36	38.00292099903527	40.0	38.0	41.0	33.0	41.0
37	37.9337817558152	40.0	37.0	41.0	33.0	41.0
38	37.89481723657413	40.0	37.0	41.0	33.0	41.0
39	37.85952406474435	40.0	37.0	41.0	33.0	41.0
40	37.783336906420836	40.0	37.0	41.0	33.0	41.0
41	37.7453103226498	40.0	37.0	41.0	33.0	41.0
42	37.66558580769643	40.0	37.0	41.0	33.0	41.0
43	36.78344409904599	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	3.0
21	9.0
22	17.0
23	23.0
24	43.0
25	55.0
26	83.0
27	127.0
28	156.0
29	241.0
30	327.0
31	475.0
32	570.0
33	847.0
34	1238.0
35	1781.0
36	2870.0
37	4900.0
38	11170.0
39	12378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81852288562547	18.429092078465	12.94618930217601	26.806195733733517
2	19.747561367777898	20.323721727945117	32.854539607674994	27.07417729660199
3	20.438953799978563	21.77082216743488	28.883052845964198	28.90717118662236
4	15.829670918640797	15.709079215349982	32.575838782291775	35.885411083717436
5	16.50766427269804	34.43295101297031	32.7473469825276	16.312037731804054
6	35.90148997748955	33.216314717547434	15.084682173866437	15.79751313109658
7	30.78840175795905	28.781219852074173	19.932468646157144	20.497909743809625
8	27.923678850895055	32.2730196162504	19.093686354378818	20.709615178475723
9	26.975024118340656	14.33969342909208	18.81230571336692	39.87297673920035
10	18.260263693857862	25.59491906956801	31.47175474327366	24.67306249330046
11	36.898381391360275	20.637260156501235	20.27548504662879	22.188873405509703
12	22.83202915639404	24.675742308929145	27.216207524922286	25.27602100975453
13	31.7290170436274	18.60863972558688	23.30635652267124	26.355986708114482
14	24.3675635116304	20.114696108907708	22.874906206452998	32.64283417300889
15	26.717761817986922	26.358666523743167	20.610462000214387	26.313109658055527
16	26.940186515167756	24.772215671561796	22.427376996462645	25.860220816807804
17	25.463608103762457	25.257262300353734	23.65741237002894	25.621717225854862
18	25.31085861292743	23.76460499517633	24.52031300246543	26.404223389430808
19	26.10140422338943	24.67306249330046	24.204094758280633	25.021438525029478
20	26.495337120806088	23.327795047700718	24.185336048879837	25.991531782613357
21	27.12241397791832	23.946832457926895	23.416228963447313	25.51452460070747
22	26.747239789902455	23.716368313860006	23.365312466502306	26.171079429735233
23	25.715510772858828	23.772644442062386	23.895915960981885	26.6159288240969
24	26.120162932790226	24.019187479901383	23.512702326079964	26.347947261228427
25	26.227355557937614	23.507342694822597	23.807482045235286	26.457819702004503
26	26.08532532961732	24.048665451816913	23.477864722907064	26.3881444956587
27	26.439060992603707	23.676171079429736	23.46982527602101	26.414942651945545
28	25.511844785078786	24.32200664594276	24.32736627720013	25.83878229177833
29	25.195626540893983	24.281809411512487	24.426519455461467	26.09604459213206
30	25.361775109872443	24.522992818094117	24.383642405402508	25.73158966663094
31	25.659234644656447	24.281809411512487	23.80212241397792	26.256833529853147
32	24.764176224675744	23.866437989066352	24.104941580019297	27.264444206238608
33	24.884767927966557	23.759245363918964	24.18265623325115	27.173330474863327
34	25.493086075677994	23.129488691178047	24.94908350305499	26.428341730088974
35	25.80930431986279	22.89366491585379	25.257262300353734	26.03976846392968
36	24.89816700610998	23.42426841033337	25.008039446886055	26.669525136670597
37	25.142030228320287	22.06292207096152	26.026369385786257	26.768678314931933
38	23.81552149212134	22.188873405509703	27.293922178154144	26.701682924214815
39	24.442598349233574	21.283095723014256	27.51902669096366	26.75527923678851
40	23.461785829134957	21.7842212455783	28.381927323400152	26.37206560188659
41	23.014256619144604	21.534998392110623	28.93396934290921	26.516775645835565
42	23.003537356629863	21.778861614320935	28.81337763961839	26.404223389430808
43	21.920891842641225	21.28041590738557	29.43241504984457	27.36627720012863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	7.0
4	13.0
5	13.0
6	10.0
7	7.0
8	6.5
9	6.0
10	10.0
11	14.0
12	14.0
13	30.0
14	46.0
15	71.0
16	96.0
17	86.0
18	76.0
19	76.0
20	108.5
21	141.0
22	163.5
23	186.0
24	223.5
25	261.0
26	261.0
27	295.0
28	329.0
29	439.0
30	549.0
31	647.0
32	745.0
33	745.0
34	859.0
35	973.0
36	1110.5
37	1248.0
38	1427.0
39	1606.0
40	1606.0
41	1827.0
42	2048.0
43	2103.5
44	2159.0
45	2272.0
46	2385.0
47	2385.0
48	2539.0
49	2693.0
50	2829.5
51	2966.0
52	3112.5
53	3259.0
54	3259.0
55	3016.0
56	2773.0
57	2763.0
58	2753.0
59	2555.5
60	2358.0
61	2358.0
62	2215.5
63	2073.0
64	1842.5
65	1612.0
66	1453.0
67	1294.0
68	1294.0
69	1114.0
70	934.0
71	813.5
72	693.0
73	554.5
74	416.0
75	416.0
76	329.0
77	242.0
78	195.0
79	148.0
80	116.0
81	84.0
82	84.0
83	67.5
84	51.0
85	47.0
86	43.0
87	33.0
88	23.0
89	23.0
90	15.0
91	7.0
92	5.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	37316.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.00686032800944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.7076687507975	77.8808018008361
2	3.665305601633278	6.1582163147175475
3	1.4578282506060993	3.6740272269267877
4	0.6730891922929693	2.261764390609926
5	0.4306494832206202	1.8088755493622037
6	0.2998596401684318	1.511416014578197
7	0.17225979328824803	1.0129703076428342
8	0.1499298200842159	1.0076106763854646
9	0.07655990812811024	0.5788401757959053
>10	0.36684955978052824	4.105477543145032
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.10451280951870512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0026798156286847465	0.0
3	0.0	0.0	0.0	0.0026798156286847465	0.0
4	0.0	0.0	0.0	0.0026798156286847465	0.0
5	0.0	0.0	0.0	0.0026798156286847465	0.0
6	0.0	0.0	0.0	0.0026798156286847465	0.0
7	0.0	0.0	0.0	0.0026798156286847465	0.0
8	0.0	0.0	0.0	0.0026798156286847465	0.0
9	0.0	0.0	0.0	0.0026798156286847465	0.0
10	0.0	0.0	0.0	0.0026798156286847465	0.0
11	0.0	0.0	0.0	0.0026798156286847465	0.0
12	0.0	0.0	0.0	0.0026798156286847465	0.0
13	0.0	0.0	0.0	0.0026798156286847465	0.0
14	0.0	0.0	0.0	0.0026798156286847465	0.0
15	0.0	0.0	0.0	0.0026798156286847465	0.0
16	0.0	0.0	0.0	0.005359631257369493	0.0
17	0.0	0.0	0.0	0.005359631257369493	0.0
18	0.0	0.0	0.0	0.005359631257369493	0.0
19	0.0	0.0	0.0	0.005359631257369493	0.0
20	0.0	0.0	0.0	0.005359631257369493	0.0
21	0.0	0.0	0.0	0.005359631257369493	0.0
22	0.0	0.0	0.0	0.005359631257369493	0.0
23	0.0	0.0	0.0	0.005359631257369493	0.0
24	0.0	0.0	0.0	0.008039446886054239	0.0
25	0.0	0.0	0.0	0.010719262514738986	0.0
26	0.0	0.0	0.0	0.016078893772108478	0.0
27	0.0	0.0	0.0	0.0401972344302712	0.0
28	0.0	0.0	0.0	0.13131096580555257	0.0
29	0.0	0.0	0.0	0.24118340658162718	0.0
30	0.0	0.0	0.0	0.41537142244613573	0.0
31	0.0	0.0	0.0	0.8575410011791189	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGACC	20	0.0018192017	37.0	22
CTCTTAT	125	2.7775604E-6	14.799999	37
TCTTATA	80	0.006149273	13.875	37
GGCTGTC	85	0.009183569	13.058823	37
TCTCTTA	190	9.440555E-8	12.657895	37
GTCTCTT	235	1.4825109E-6	10.234043	36
TGTCTCT	295	2.6616193E-5	8.152543	35
CTGTCTC	380	1.19176E-6	7.789474	37
>>END_MODULE