FastQCFastQC Report
Fri 10 Feb 2017
ERR1632061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26717
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT780.29194894636373847TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC310.11603099150353709No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC300.11228805629374555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT450.003730245220.55555537
AACGCAG500.00686082518.4999985
ACGCAGA500.00686082518.4999986
TCAACGC500.00686082518.4999983
CAGAGTA500.00686082518.4999989
GCAGAGT500.00686082518.4999988
TATCAAC500.00686082518.4999981
ATCAACG500.00686082518.4999982
TGTCTCT1451.2570921E-512.75862137
CTGTCTC1758.605549E-510.57142936
Produced by FastQC (version 0.11.5)