##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26717
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503162780252275	31.0	31.0	34.0	30.0	34.0
2	31.711756559493956	31.0	31.0	34.0	30.0	34.0
3	31.76389564696635	31.0	31.0	34.0	30.0	34.0
4	35.54553280682711	37.0	35.0	37.0	33.0	37.0
5	34.78758842684433	37.0	35.0	37.0	32.0	37.0
6	34.72268593030655	35.0	35.0	37.0	32.0	37.0
7	34.730471235542915	35.0	35.0	37.0	32.0	37.0
8	34.68884979601003	35.0	35.0	37.0	31.0	37.0
9	36.29703933824906	37.0	35.0	39.0	32.0	39.0
10	36.06527679005877	37.0	35.0	39.0	32.0	39.0
11	36.24504996818505	37.0	35.0	39.0	32.0	39.0
12	36.10850769173186	37.0	35.0	39.0	32.0	39.0
13	36.219298573941686	37.0	35.0	39.0	32.0	39.0
14	37.19736497361231	38.0	36.0	40.0	32.0	41.0
15	37.20275480031441	38.0	36.0	40.0	32.0	41.0
16	37.15095257701089	38.0	36.0	40.0	32.0	41.0
17	37.078676498109814	38.0	36.0	40.0	32.0	41.0
18	37.09581914137066	38.0	36.0	40.0	32.0	41.0
19	37.10285585956507	39.0	36.0	40.0	32.0	41.0
20	37.02840887824232	39.0	36.0	40.0	32.0	41.0
21	37.008646180334615	39.0	36.0	40.0	31.0	41.0
22	36.95186585320208	38.0	36.0	40.0	31.0	41.0
23	36.885391323876185	38.0	36.0	40.0	31.0	41.0
24	36.7952988733765	38.0	36.0	40.0	31.0	41.0
25	36.6821873713366	38.0	36.0	40.0	31.0	41.0
26	36.51435415652955	38.0	35.0	40.0	30.0	41.0
27	36.44144177864281	38.0	35.0	40.0	30.0	41.0
28	36.32245386832354	38.0	35.0	40.0	30.0	41.0
29	36.17861286821125	38.0	35.0	40.0	30.0	41.0
30	36.08346745517835	38.0	35.0	40.0	30.0	41.0
31	36.00329378298461	38.0	35.0	40.0	29.0	41.0
32	35.90208481491185	38.0	34.0	40.0	29.0	41.0
33	35.75809409739117	38.0	34.0	40.0	29.0	41.0
34	35.62316128307819	38.0	34.0	40.0	28.0	41.0
35	35.60085338922783	38.0	34.0	40.0	28.0	41.0
36	35.54089156716697	38.0	34.0	40.0	28.0	41.0
37	35.489089343863455	38.0	34.0	40.0	28.0	41.0
38	35.44428640940225	38.0	34.0	40.0	27.0	41.0
39	35.310551334356404	38.0	34.0	40.0	27.0	41.0
40	35.20739603997455	38.0	33.0	40.0	27.0	41.0
41	35.14586218512557	38.0	33.0	40.0	27.0	41.0
42	35.09394767376577	38.0	33.0	40.0	27.0	41.0
43	34.17427106336789	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	5.0
20	11.0
21	23.0
22	22.0
23	48.0
24	75.0
25	119.0
26	195.0
27	245.0
28	342.0
29	512.0
30	690.0
31	906.0
32	1198.0
33	1615.0
34	2206.0
35	3005.0
36	3961.0
37	4948.0
38	4881.0
39	1705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.092413070329755	18.24306621252386	11.891305161507654	26.77321555563873
2	21.952315005427256	20.327881124377736	30.100684957143393	27.619118913051615
3	22.064603061721	20.866863794587715	27.73140696934536	29.337126174345922
4	16.918067148257666	15.855073548676874	30.501179024591085	36.725680278474385
5	17.70034060710409	33.05760377287869	31.758805255081036	17.483250364936183
6	37.6876146273908	31.960923756409777	13.923718980424448	16.427742635774976
7	31.234794325710226	27.802522738331398	19.22745817269903	21.735224763259346
8	29.277239210989258	30.418834449975673	18.325410787139273	21.978515551895796
9	27.020249279484972	13.48205262566905	17.98480368304825	41.51289441179773
10	19.279859265636112	24.508739753714863	31.227308455290636	24.98409252535839
11	39.38690721263615	19.64666691619568	18.621102668712805	22.345323202455365
12	23.359658644308865	23.576748886476775	26.481266609274996	26.582325859939367
13	33.6714451472845	17.77519931129992	21.41707527042707	27.13628027098851
14	24.74828760714152	19.35097503462215	21.918628588539132	33.98210876969719
15	28.072014073436392	24.77448815361006	19.732754426020886	27.420743346932664
16	27.94475427630348	23.183740689448666	21.772654115357263	27.098850918890594
17	26.387693229030205	24.164389714414046	22.420181906651194	27.02773514990455
18	26.645955758505817	22.611071602350563	23.176254819029083	27.566717820114533
19	27.372085189205375	23.565520080847403	22.506269416476403	26.556125313470822
20	27.637833589100573	21.851255754762885	23.123853726092	27.38705693004454
21	28.783171763296778	22.21057753490287	22.169405247595165	26.83684545420519
22	28.259160833925968	22.962907512070966	21.821312273084555	26.956619380918518
23	27.417000411722874	22.92173522476326	22.195605794063706	27.465658569450163
24	28.251674963506378	22.25923569263016	22.094546543399336	27.394542800464123
25	27.252311262492046	22.442639517909946	22.60358573193098	27.701463487667027
26	27.45817269903058	23.04525208668638	22.753303140322643	26.7432720739604
27	27.21113897518434	22.888048807875137	22.506269416476403	27.394542800464123
28	26.70584272186248	22.614814537560353	23.007822734588466	27.671520005988697
29	26.608526406407908	22.742074334693267	23.3446869034697	27.304712355429128
30	26.219261144589588	22.873077067035968	23.51311898791032	27.394542800464123
31	27.154994947037466	22.622300407979935	22.543698768574316	27.67900587640828
32	26.664670434554775	22.89927761350451	22.79447542763035	27.641576524310363
33	25.83373881798106	22.55867050941348	23.876183703260097	27.73140696934536
34	27.454429763820787	22.596099861511398	23.019051540217838	26.930418834449977
35	26.668413369764572	22.510012351686193	24.10450275105738	26.71707152749186
36	27.102593854100387	22.510012351686193	22.506269416476403	27.88112437773702
37	26.417636710708535	21.757682374518097	24.21304787214133	27.611633042632032
38	26.144402440393755	21.162555676161247	24.98409252535839	27.70894935808661
39	25.669049668750233	21.211213833888536	25.811281206722313	27.308455290638918
40	25.713964891267732	20.889321405846463	26.589811730358946	26.806901972526852
41	25.33967137028858	21.506905715462064	25.871168170078978	27.28225474417038
42	24.6247707452184	20.99786652693042	26.612269341617694	27.765093386233485
43	23.93981360182655	20.698431710147098	27.454429763820787	27.90732492420556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	4.0
7	6.0
8	5.0
9	4.0
10	6.0
11	8.0
12	8.0
13	9.5
14	11.0
15	15.0
16	19.0
17	21.0
18	23.0
19	23.0
20	29.5
21	36.0
22	37.0
23	38.0
24	47.5
25	57.0
26	57.0
27	81.5
28	106.0
29	155.5
30	205.0
31	245.0
32	285.0
33	285.0
34	371.5
35	458.0
36	537.0
37	616.0
38	749.5
39	883.0
40	883.0
41	989.0
42	1095.0
43	1267.0
44	1439.0
45	1557.0
46	1675.0
47	1675.0
48	1854.0
49	2033.0
50	2090.0
51	2147.0
52	2201.5
53	2256.0
54	2256.0
55	2323.5
56	2391.0
57	2325.5
58	2260.0
59	2168.5
60	2077.0
61	2077.0
62	1971.0
63	1865.0
64	1659.5
65	1454.0
66	1263.5
67	1073.0
68	1073.0
69	944.0
70	815.0
71	695.5
72	576.0
73	452.5
74	329.0
75	329.0
76	264.5
77	200.0
78	152.5
79	105.0
80	91.0
81	77.0
82	77.0
83	62.5
84	48.0
85	39.0
86	30.0
87	20.0
88	10.0
89	10.0
90	7.5
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26717.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.42804207059176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.35109743257472	88.1311524497511
2	3.2680003239653357	6.0410974286035115
3	0.7937150724872438	2.2008459033574126
4	0.25917226856726333	0.9581914137066287
5	0.11743743419454118	0.5427256054197702
6	0.06884263383817932	0.3817793913987349
7	0.04454523365999838	0.2882060111539469
8	0.016198266785453958	0.1197739267133286
9	0.012148700089090468	0.101059250664371
>10	0.06479306714181583	0.9432196728674627
>50	0.0040495666963634895	0.29194894636373847
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	78	0.29194894636373847	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	31	0.11603099150353709	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	30	0.11228805629374555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0037429352097915185	0.0
26	0.0	0.0	0.0	0.011228805629374556	0.0
27	0.0	0.0	0.0	0.02245761125874911	0.0
28	0.0	0.0	0.0	0.04865815772728974	0.0
29	0.0	0.0	0.0	0.11228805629374555	0.0
30	0.0	0.0	0.0	0.17591795486020137	0.0
31	0.0	0.0	0.0	0.3630647153497773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAT	45	0.0037302452	20.555555	37
AACGCAG	50	0.006860825	18.499998	5
ACGCAGA	50	0.006860825	18.499998	6
TCAACGC	50	0.006860825	18.499998	3
CAGAGTA	50	0.006860825	18.499998	9
GCAGAGT	50	0.006860825	18.499998	8
TATCAAC	50	0.006860825	18.499998	1
ATCAACG	50	0.006860825	18.499998	2
TGTCTCT	145	1.2570921E-5	12.758621	37
CTGTCTC	175	8.605549E-5	10.571429	36
>>END_MODULE